Description
For SampleFor the quantitative determination of human carbohydrate-deficient transferring (CDT) concentrations in serum, plasma.
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CDT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for CDT. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CDT bound in the initial step. The color development is stopped and the intensity of the color is measured