Description
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to alpha2 MG has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with HRP-conjugated alpha2 MG and incubated. A competitive inhibition reaction is launched between alpha2 MG (Standards or samples) and HRP-conjugated alpha2 MG with the pre-coated antibody specific for alpha2 MG. The more amount of alpha2 MG in samples, the less antibody bound by HRP-conjugated alpha2 MG. Then the substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of alpha2 MG in the sample. The color development is stopped and the intensity of the color is measured