Description
Intended Use The anti-spermatozoa antibody ELISA from MBS is a reliable test for the determination antibodies directed against human spermatozoa. This test is intended for the use with seminal plasma. Please note: the terms “anti-spermatozoa antibodies”, “anti-sperm antibodies” and “sperm antibodies” are equivalent. In these instructions the rather unwieldy but correct term “anti-spermatozoa antibodies” is used. For Research Use Only – Not for Use in Diagnostic Procedure
PRINCIPLE OF THE TEST The anti-spermatozoa antibody ELISA (Enzyme Linked ImmunoSorbent Assay) from MBS is a solid-phase sandwich enzyme-immunoassay for the determination of anti-spermatozoa antibodies in human seminal plasma. The ELISA-plate is coated with a mix of spermatozoa proteins which are recognized by antispermatozoa antibodies. The samples and standards are pipetted into the wells and then incubated. During this incubation anti-spermatozoa antibodies bind to the spermatozoa proteins and are thus immobilised on the plate. After washing the enzyme conjugate, consisting of anti-human globulin antibodies covalently coupled to horseradish peroxidase, is added. After removal of the unbound conjugate by washing the horseradish peroxidase oxidizes the then added substrate TMB (3,3’,5,5’- tetramethylbenzidine) yielding a colour reaction which is stopped with 0.5 N acidic solution. The extinction is measured at a wavelength of 450 nm with a microplate reader. The use of a reference measurement with a wavelength ?550 nm is recommended