Description
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to LTF/LF has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with HRP-conjugated LTF/LF and incubated. A competitive inhibition reaction is launched between LTF/LF (Standards or samples) and HRP-conjugated LTF/LF with the pre-coated antibody specific for LTF/LF. The more amount of LTF/LF in samples, the less antibody bound by HRP-conjugated LTF/LF. Then the substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of LTF/LF in the sample. The color development is stopped and the intensity of the color is measured