Amyloid Beta (1-42) ELISA 27711 from IBL- America

Product Specs
ItemAmyloid Beta (1-42) ELISA
CompanyIBL- America
Price $734.00Supplier Page View Company Product Page
Catalog Number27711
Quantity96 Determinations
Sample TypeCerebrospinal fluids, cell culture media, extract from brain tissue
Detection Range0 / 12.5 - 800 pg/mL
ApplicationsELISA Kit
ReactivityHuman
Sensitivity4.03 pg/mL
Volume100 µL of properly diluted unknown / determination
Regulatory StatusFor research use only, not for use in diagnostic procedures
Assay TimeOvernight incubation (4°C) + 1 hour (4°C) + 30 min. (RT) = Overnight + 1 hour, 30 min. total incubation time
Storage2 - 8 °C

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IBL- America

8201 Central Ave NE Suite P

Minneapolis, Minnesota 55432

United States

Phone: 888-523-1246
763-780-2988

Company Profile

Website: http://www.ibl-america.com/

(1) Human brain and organoid transcriptomes reveal key receptor tyrosine kinase pathways and genetic signatures in Alzheimer's diseaseExperimental & Molecular MedicineApril 15, 2026Saewoon Shin, Xiaohui Zhu, Sarnai Amartumur, Taehoon Lee, Won Jong Yu, Soomin Park, Niloofar Etemadi, Ariunzaya Jamsranjav, Rian Kang, Gyusoo Bak, et al.generation of brain organoids (IRB no. SKKU IRB 2024-10-020). The levels of beta-amyloids (1–42, cat: 27711, IBL; 1–40, cat: 27713, IBL), phosphorylated tau (pT181, cat: KHO0631, Invitrogen), and total tau (KHB0041,

(2) De-2-hydroxyisobutyrylation of clusterin modulates neuropathology and memory deficits in an Alzheimer’s disease mouse modelCell Communication and SignalingMarch 9, 2026Xiaoping Peng, Chaoyue Ouyang, Fubin Ma, Yu Liu, Lv Luo, Jiawei Xing, Sifan Feng, Li Li, Jiahao Zhang, Jiaying Wang, et al.and insoluble Aβ40 and Aβ42 were quantified using ELISA kits (IBL, Japan, Code No. 27713 and Code No. 27711). The levels of the cytokines IL-6, TNF-α, and TGF-β1 were determined using the respective ELISA kits

(3) SGLT2 Inhibition by Enavogliflozin Significantly Reduces Aβ Pathology and Restores Cognitive Function via Upregulation of Microglial AMPK Signaling in 5XFAD Mouse Model of Alzheimer's DiseaseAging CellMay 10, 2025Jihui Han, Jaehoon Song, Eun Sun Jung, Ji Won Choi, Hye Young Ji, Inhee Mook‐Jungenzyme‐linked immunosorbent assay (ELISA) kits, according to the manufacturer's protocols (IBL 27713, 27711). Concentrations of TNFα and IL‐1β were determined using assay kits from R&D Systems (MTA00B, MLB00C)

(4) Commonly prescribed multi-medication therapies exert sex-specific effects on Alzheimer's disease pathology and metabolomic profiles in AppNL-G-F mice: Implications for personalized therapeutics in agingAlzheimers DementMarch 27, 2025Francesca Eroli, Kristina Johnell, Zeynep Acararicin, Christina Tsagkogianni, Stefania Zerial, Saverio Lancia, Maria Latorre-Leal, Vilma Alanko, Sarah N. Hilmer, Anna Matton, et al.0.5 M. We determined the levels of Aβ40 and Aβ42 in both fractions by using ELISA kits #27713 and #27711 (IBL, Japan) according to kit instructions. 2.7 Western blotting We ran western blot experiments on

(5) 25-Hydroxycholesterol modulates microglial function and exacerbates Alzheimer's disease pathology: mechanistic insights and therapeutic potential of cholesterol esterification inhibitionJ NeuroinflammationFebruary 25, 2025Hayoung Choi, Haeng Jun Kim, Sang-Eun Lee, Hyun Ho Song, Jieun Kim, Jihui Han, June-Hyun Jeong, Do Yup Lee, Sunghoe Chang, Inhee Mook-Jung1:20 (v/v) and used in subsequent experiments. ELISA assay was performed using Abeta(1–42) ELISA kit (#27711, IBL) according to the test method provided by the manufacturer. GC-MS analysis Metabolites extraction

(6) Pathway-based network medicine identifies novel natural products for Alzheimer's diseaseAlzheimers Res TherFebruary 14, 2025Yumei Liang, Siqi Xie, Jianping JiaBiotech (Suzhou, China). Enzyme-linked immunosorbent assay (ELISA) Kits for Aβ1−40 (27713), Aβ1−42 (27711) and Aβ oligomers (27725) were purchased from Immuno-Biological Laboratories (Fujioka, Japan). The RNAsimple

(7) A Novel RAGE Modulator Induces Soluble RAGE to Reduce BACE1 Expression in Alzheimer's DiseaseAdvanced ScienceJanuary 4, 2025Seung‐Hyun Baek, Suji Hong, Eunae Kim, Sunyoung Park, Minyoung Lee, Jinsu Park, Yoonsuk Cho, Hyunjun Yoon, Daeseung Kim, Youngkwang Yun, et al.quantified using a solid‐phase sandwich ELISA system (IBL International, Cat No. 27713 for Aβ40 and 27711 for Aβ42). Standards and brain extracts were incubated on a microtiter plate precoated with a monoclonal

(8) Metabolic resistance of Aβ3pE-42, a target epitope of the anti-Alzheimer therapeutic antibody, donanemabLife Science AllianceDecember 1, 2024Nobuhisa Iwata, Satoshi Tsubuki, Misaki Sekiguchi, Kaori Watanabe-Iwata, Yukio Matsuba, Naoko Kamano, Ryo Fujioka, Risa Takamura, Naoto Watamura, Naomasa Kakiya, et al.Fujifilm Wako #292-62301, Fujifilm Wako #298-62401, and Immuno-Biological Laboratories [IBL] #27713, #27711, #27418, and #27716, respectively. Autoradiographic and immunohistochemical labeling and PET imaging Radiosynthesis

(9) The deficient CLEC5A ameliorates the behavioral and pathological deficits via the microglial Aβ clearance in Alzheimer’s disease mouse modelJournal of NeuroinflammationOctober 23, 2024Yu-Yi Lin, Wen-Han Chang, Shie-Liang Hsieh, Irene Han-Juo Chengand protease inhibitor (04693116001; Roche) before applying to total Aβ and Aβ1−42 ELISA kits (27729, 27711; IBL). For measuring the murine IL-1β, the mice hippocampal tissues were homogenized in RIPA buffer

(10) Specific Mutations near the Amyloid Precursor Protein Cleavage Site Increase γ-Secretase Sensitivity and Modulate Amyloid-β ProductionInternational Journal of Molecular SciencesFebruary 16, 2023Ryota Suzuki, Haruka Takahashi, Chika Yoshida, Masafumi Hidaka, Tomohisa Ogawa, Eugene Futai(Human Amyloidβ [1–40] Assay Kit [#27713; IBL, Gunma, Japan] and Human Amyloidβ [1–42] Assay Kit [#27711; IBL]). The amounts of Aβ secreted were also measured in the media and cells, which were subjected to

(11) Targeting PSEN1 by lnc-CYP3A43-2/miR-29b-2-5p to Reduce β Amyloid Plaque Formation and Improve Cognition FunctionInternational Journal of Molecular SciencesSeptember 11, 2022Wei Wuli et al.These supernatants were stored at −80 °C. They were analyzed by sandwich ELISA (IBL-international, 27711, Hamburg, Germany), and Aβ42 quantitation were carried out within 30 min (Thermo Scientific, Multiskan

(12) KIBRA regulates amyloid β metabolism by controlling extracellular vesicles secretioneBioMedicineApril 1, 2022Xiaolei Han et al.sandwich enzyme-linked immunosorbent assay (ELISA) using commercially available kits (27713 for Aβ40 and 27711 for Aβ42, Immuno-Biological Laboratories (IBL), Co., Ltd., Fujioka, Japan) following the manufacturer's

(13) A logical network-based drug-screening platform for Alzheimer’s disease representing pathological features of human brain organoidsNature CommunicationsDecember 1, 2021Jong-Chan Park et al.supernatant was collected and stored at −80 °C. The proteins levels of Aβ1-40 (27713, IBL), Aβ1-42 (27711, IBL), phospho-tau (pT181) (KHO0631, Invitrogen), and total-tau (KHB0041, Invitrogen) were measured

(14) Repeated electromagnetic field stimulation lowers amyloid-β peptide levels in primary human mixed brain tissue culturesScientific ReportsDecember 1, 2021Felipe P. Perez, Bryan Maloney, Nipun Chopra, Jorge J. Morisaki, Debomoy K. Lahiriof Aβ40 and Aβ42 were measured using specific ELISA kits obtained from IBL (catalogue #s 27713 and 27711, respectively), and the assay was performed as per the manufacturer’s protocol. Briefly, an equal volume

(15) Reduction of Amyloid Burden by Proliferated Homeostatic Microglia in Toxoplasma gondii-Infected Alzheimer’s Disease Model MiceInternational Journal of Molecular SciencesMarch 9, 2021Ji-Hun Shin, Young Sang Hwang, Bong-Kwang Jung, Seung-Hwan Seo, Do-Won Ham, Eun-Hee Shincells around plaques. 4.7. ELISA for Amyloid β (1–42) Quantification The Amyloid β (1–42) Assay Kit (#27711, IBL, Tokyo, Japan) was used to measure amounts of Aβ42 (n = 7 per group). The test sample included

(16) Dynamic Blood Concentrations of Aβ1–40 and Aβ1–42 in Alzheimer’s DiseaseFrontiers in Cell and Developmental BiologyAugust 11, 2020Yuan-Han Yang, Ling-Chun Huang, Sun-Wung Hsieh, Li-Ju Huangβ(1–40) Assay Kit – IBL, code number 27713; and Human Amyloid β(1–42) Assay Kit – IBL, code number 27711). All assays were performed according to the manufacturer’s protocol. All reagents were prepared at

(17) Evaluation of Toxic Amyloid β42 Oligomers in Rat Primary Cerebral Cortex Cells and Human iPS-derived Neurons Treated with 10-Me-Aplog-1, a New PKC ActivatorInternational Journal of Molecular SciencesFebruary 11, 2020Kazuma Murakami, Mayuko Yoshimura, Shota Nakagawa, Toshiaki Kume, Takayuki Kondo, Haruhisa Inoue, Kazuhiro IrieLines Treated amountLevels of Aβ42 secreted by SH-SY5Y cells was with near1 to the detection limit of specific ELISA (#27711 Human Amyloidon β 1-42 Kit—IBL), selectedin ratthe primary cerebral cortex cells The reason why research PKCAssay modulators faces

(18) Effects of ovarian hormone loss on neuritic plaques and autophagic flux in the brains of adult female APP/PS1 double-transgenic miceActa biochimica et biophysica SinicaMay 1, 2018Yao Q, Feng M, Yang B, Long Z, Luo S, Luo M, He G, Wang K.Aβ40 or Aβ42 was detected by an Amyloid β (1–40) or Amyloid β (1–42) Colorimetric ELISA Kit (27713 or 27711; Immuno-Biological Laboratories Co., Ltd, Fujioka, Japan) according to the manufacturer’s instructions.

(19) Vitamin D3-Enriched Diet Correlates with a Decrease of Amyloid Plaques in the Brain of AβPP Transgenic MiceJournal of Alzheimer's DiseaseMarch 24, 2018Jin Yu, Marco Gattoni-Celli, Hong Zhu, Narayan R. Bhat, Kumar Sambamurti, Sebastiano Gattoni-Celli, Mark S. Kindylevels of human Aβ40 and Aβ42 [ImmunoBiological Laboratories-America (IBL, catalog # 27718 for 1–40 and 27711 for 1–42)]. Aβ40 and Aβ42 were extracted from mouse brains as follows: frozen hemi-brains were placed

(20) Amelioration of amyloid-β-induced deficits by DcR3 in an Alzheimer's disease modelMolecular neurodegenerationApril 24, 2017Liu YL, Chen WT, Lin YY, Lu PH, Hsieh SL, Cheng IH.Basel, Switzerland). The levels of total Aβ and Aβ42 were quantified using Aβ ELISA kits (27729 and 27711, IBL, Hamburg, Germany). For cytokines measurement diluted hippocampus lysates and conditioned media

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Amyloid Beta (1-42) ELISA from IBL- America

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Amyloid Beta (1-42) ELISA from IBL- America

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