Description
Introduction: Type III collagen has been suggested to play a major role in development and wound healing, and contributestothe viscoelastic properties of mostconnective tissues. Type III Collagen is found co-localized with Type I Collagen in tissues, blood vessels and skin. Type III Collagen is a homotrimeric procollagen comprised of three identical pro alpha1 (III) chains. Collagen III is important for the development of skin and the cardiovascular system and for maintaining normal physiological functions in adult life. Collagen III has been used for studies of Collagen I fibrillogenesis in normal cardiovascular development. Many of the same properties and functions of animal derived collagens can be duplicated using recombinant human collagens
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Col III. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Col III and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Col III, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Col III in the samples is then determined by comparing the O.D. of the samples to the standard curve