Description
Principle of the Assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. An antibody specific for FT4 has been pre-coated onto a microplate. Add FT4 (Standards or samples) to the well, and then add Biotin conjugated FT4. A competitive inhibition reaction is launched between FT4 (Standards or samples) and Biotin conjugated FT4 with the FT4 antibody. Then add the HRP-avidin to each well. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of FT4 bound in the initial step. The color development is stopped and the intensity of the color is measured