Description
Intended Use The MBS Varizella zoster IgM Antibody ELISA Test Kit has been designed for the detection of IgM class antibodies against Varizella zoster in serum and plasma. For research use only, not for use in diagnostic procedures.
Principle of the test The MBS Varizella zoster IgM antibody test kit is based on the principle of the enzyme immunoassay (EIA). Varizella zoster antigen is bound on the surface of the microtiter strips. Diluted unknowns are pipetted into the wells of the microtiter plate. A binding between the IgM antibodies of the serum and the immobilized Varizella zoster antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then readyto- use anti-human-IgM peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. NOTE that unknowns containing abnormally elevated levels of anti-human IgG antibodies may cause inaccurate results in this assay. Any unknowns showing elevated results should be pre-diluted with IgG adsorbent and re-tested to confirm elevated levels of IgM