Description
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibodyspecific to EGF. Standards or samples are then added to the appropriatemicrotiter plate wells with a biotin-conjugated antibody preparation specific forEGF and Avidin conjugated to Horseradish Peroxidase (HRP) is added toeach microplate well and incubated. Then a TMB (3,3',5,5'tetramethyl-benzidine) substrate solution is added to each well. Only thosewells that contain EGF, biotin-conjugated antibody and enzyme-conjugatedAvidin will exhibit a change in color. The enzyme-substrate reaction isterminated by the addition of a sulphuric acid solution and the color change ismeasured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. Theconcentration of EGF in the samples is then determined by comparing the O.D.of the samples to the standard curve