Description
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CoQ10. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated CoQ10. The competitive inhibition reaction is launched between with HRP-conjugated CoQ10 and CoQ10 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of CoQ10 in the sample. The color development is stopped and the intensity of the color is measured