Description
For Sample:For the quantitative determination of goat immunoglobulin A (IgA) concentrations in serum, plasma.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat IgG. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for goat IgG. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of goat IgG in samples. The color development is stopped and the intensity of the color is measured