Description
Introduction: Adiponectin (also referred to as GBP-28, apM1, AdipoQ and Acrp30) is a protein is encoded by the ADIPOQ gene. Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid catabolism. Adiponectin is exclusively secreted from adipose tissue into the bloodstream and is very abundant in plasma relative to many hormones. Levels of the hormone are inversely correlated with body fat percentage in adults, while the association in infants and young children is less clear. The hormone plays a role in the suppression of the metabolic derangements that may result in type 2 diabetes, obesity, atherosclerosis, non-alcoholic fatty liver disease (NAFLD) and an independent risk factor for metabolic syndrome.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A antibody specific to adiponectin has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated adiponectin and incubated. A competitive inhibition reaction is launched between adiponectin (Standards or samples) and Biotin-conjugated adiponectin with the pre-coated antibody specific for adiponectin. The more amount of adiponectin in samples, the less antibody bound by Biotin-conjugated adiponectin. Then Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of adiponectin in the sample. The color development is stopped and the intensity of the color is measured