Description
For samples: For the quantitative determination of rat lecithin cholesterol acyltransferase (LCAT) concentrations in serum, plasma, tissue homogenates.
PRINCIPLE OF THE ASSAY This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LCAT. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for LCAT. The competitive inhibition reaction is launched between with pre-coated LCAT and LCAT in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of LCAT in the sample. The color development is stopped and the intensity of the color is measured