Description
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAM33 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAM33 present is bound by the immobilized antibody. After removing anyunbound substances, a biotin-conjugated antibody specific for ADAM33 is added to the wells.After washing,avidin conjugated Horseradish Peroxidase (HRP) is added to the wells.Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added tothe wells and color develops in proportion to the amount of ADAM33 bound in the initial step. The colordevelopment is stopped and the intensity of the color is measured