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Assay Kits
Assay Kit
Caspase-1 Assay Kit (Fluorometric)
Caspase-1 Assay Kit (Fluorometric) from Abcam
Be the first to write a review!
Citations:
(23)
Supplier Page
Supplier Page from
Abcam for
Caspase-1 Assay Kit (Fluorometric)
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Caspase-1 Assay Kit (Fluorometric)
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab39412
Quantity
100 test
Sample Type
Tissue Extracts, Cell Lysate
Assay Time
2h 0m
Applications
Cellular Assay
Gene Name
CASP1
Type
Enzyme activity
NCBI Gene Aliases
ICE, IL1BC, P45
Detection Target
Caspase-1
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (23)
(1)
Polyphenol-rich diet mediates interplay between macrophage-neutrophil and gut microbiota to alleviate intestinal inflammation
Cell Death & Disease
October 9, 2023
Dandan Han, Yujun Wu, Dongdong Lu, Jiaman Pang, Jie Hu, Xiangyu Zhang, Zhenyu Wang, Guolong Zhang, Junjun Wang
Caspase-1 and NLRP3 activation in colonic and cellular lysis was measured using Caspase-1 Assay Kit (
ab39412
,
Abcam
, Cambridge, MA, USA) and NLRP3 ELISA Kit (ab279417, Abcam, Cambridge, MA, USA), respectively,
(2)
Enhanced Activity of NLRP3 Inflammasome in the Lung of Patients with Anti-Synthetase Syndrome
Cells
December 23, 2022
Espiridión Ramos-Martinez, Angel E. Vega-Sánchez, Gloria Pérez-Rubio, Mayra Mejia, Ivette Buendía-Roldán, Montserrat I. González-Pérez, Heidegger N. Mateos-Toledo, Warrison A. Andrade, Ramcés Falfán-Valencia, Jorge Rojas-Serrano
According to the manufacturer’s instructions, the activity was detected using a Caspase-1 assay kit (
ab39412
,
Abcam
™, Cambridge, UK). All
assays
were performed in triplicate in three independent experiments. 2.7.
(3)
Synthetic Secoisolariciresinol Diglucoside (LGM2605) Prevents Asbestos-Induced Inflammation and Genotoxic Cell Damage in Human Mesothelial Cells
International Journal of Molecular Sciences
September 3, 2022
Ralph A. Pietrofesa, Shampa Chatterjee, Yuwaraj Kadariya, Joseph R. Testa, Steven M. Albelda, Melpo Christofidou-Solomidou
activity was determined using a commercially available fluorometric
assay
kit (Caspase-1 Assay Kit,
ab39412
,
Abcam
, Waltham, MA, USA), as previously performed [15]. Briefly, the
assay
kit
measures
caspase-1 activity
(4)
Complanatuside alleviates inflammatory cell damage induced by pro-inflammatory cytokines in skin keratinocytes
Frontiers in Chemistry
August 10, 2022
Xiao Wang et al.
fluorescence microscope
. For the caspase-1 activity
assay
, the
Caspase-1 assay kit (Fluorometric
) (#
ab39412
,
Abcam
) was used. In brief, cell lysates were prepared
after treatment
. Reaction buffer and YVAD-AFC
(5)
Galacto-oligosaccharides alleviate lung inflammation by inhibiting NLRP3 inflammasome activation in vivo and in vitro
Journal of advanced research
July 1, 2022
Cai Y, Gilbert MS, Gerrits WJJ, Folkerts G, Braber S.
assay
Caspase-1 activity in PBECs and
A549 cells
was determined in 50 μL cell lysates using a commercial kit (
ab39412
,
Abcam
, Cambridge, UK) according to the manufacturer’s instructions. For the in vivo experiments, same
(6)
MicroRNA-1224-5p Aggravates Sepsis-Related Acute Lung Injury in Mice
Oxidative Medicine and Cellular Longevity
June 28, 2022
Bing Liu, Feng Chen, Ni-Tao Cheng, Zheng Tang, Xian-Guo Wang, Ming Xu
(
ab102526
), myeloperoxidase (MPO) activity assay kit (
ab105136
), and caspase-1 activity assay kit (
ab39412
) were all purchased from
Abcam
(Cambridge, MA, USA). TransAM® NF-κB p65 kit (40097) was purchased from
(7)
Role of Polyphenol-Derived Phenolic Acid in Mitigation of Inflammasome-Mediated Anxiety and Depression
Biomedicines
May 28, 2022
Ruth Iban-Arias, Maria Sebastian-Valverde, Henry Wu, Weiting Lyu, Qingli Wu, Jim Simon, Giulio Maria Pasinetti
caspase-1 (Cat#
ab39901
,
Abcam
, Cambridge, UK), and the fluorometric Caspase-1 Assay Kit (Abcam, Cat#
ab39412
,
Abcam
, Cambridge, UK), by following the protocol established by the fabricator. 2.1.9. THP1
Macrophages
THP1
(8)
RGS6 Drives Spinal Cord Injury by Inhibiting AMPK Pathway in Mice
Disease markers
January 1, 2022
Dao W, Xiao Z, Yang W, Luo X, Xia H, Lu Z.
and tumor necrosis factor-alpha (TNF-α,
ab208348
) ELISA kits, and fluorometric caspase-1 assay kit (
ab39412
) were purchased from
Abcam
(Cambridge, MA, USA). Lentiviral vectors carrying short hairpin RNA against
(9)
TRPV1 Hyperfunction Contributes to Renal Inflammation in Oxalate Nephropathy
International Journal of Molecular Sciences
June 8, 2021
Chien-Lin Lu, Te-Yi Teng, Min-Tser Liao, Ming-Chieh Ma
in cell lysates and renal cortical tissues was determined using a commercial fluorometric
assay
kit (
ab39412
) obtained from
Abcam
(Bristol, UK) and expressed as units/mg of protein. 4.16. Statistics Numerical
(10)
Deubiquitination of NLRP6 inflammasome by Cyld critically regulates intestinal inflammation
Nature Immunology
June 1, 2020
Sandip Mukherjee, Ritesh Kumar, Elviche Tsakem Tsakem Lenou, Venkatesha Basrur, Dimitris L. Kontoyiannis, Fotis Ioakeimidis, George Mosialos, Arianne L. Theiss, Richard A. Flavell, K. Venuprasad
activity
assay
. The activity of caspase-1 was determined in colon homogenates by a commercial kit (
Abcam
,
ab39412
) according to the manufacturer’s instructions. Briefly, fresh
frozen sections
of colons were homogenized
(11)
Haematopoietic stem cell gene therapy with IL ‐1Ra rescues cognitive loss in mucopolysaccharidosis IIIA
EMBO Molecular Medicine
March 6, 2020
Helen Parker et al.
1 activity was measured using 100 μg total brain protein with the Caspase 1 Fluorometric Assay Kit (
ab39412
) as per the manufacturer's protocol (
Abcam
, UK), using recombinant
mouse
caspase 1 (0.02‐2U; Abcam)
(12)
Tojapride prevents CaSR-mediated NLRP3 inflammasome activation in oesophageal epithelium irritated by acidic bile salts
Journal of cellular and molecular medicine
January 1, 2020
Yin XL, Wu HM, Zhang BH, Zhu NW, Chen T, Ma XX, Zhang LY, Lv L, Zhang M, Wang FY,
et al
.
Measurement of caspase‐1 activity The activity of caspase‐1 was detected using a Caspase 1 Assay kit (
ab39412
,
Abcam
) per the manufacturer's instructions. All
assays
were performed in triplicate in three independent
(13)
The pore-forming subunit Kir6.1 of the K-ATP channel negatively regulates the NLRP3 inflammasome to control insulin resistance by interacting with NLRP3
Experimental & molecular medicine
August 6, 2019
Du RH, Lu M, Wang C, Ding JH, Wu G, Hu G.
pcDNA3-N-Flag-NLRP3 (75127) was obtained from Addgene (Watertown, MA, USA). A caspase-1 activity assay kit (
ab39412
) was obtained from
Abcam
(Cambridge, UK). All other materials were obtained as described elsewhere22.
Mice
Kir6.1
(14)
Immunoglobulin light chains generate proinflammatory and profibrotic kidney injury
The Journal of clinical investigation
June 17, 2019
Ying WZ, Li X, Rangarajan S, Feng W, Curtis LM, Sanders PW.
Millipore Sigma
); a Caspase-1 Colorimetric Assay Kit (
Ab39470
,
Abcam
) or a Caspase-1 Fluorometric Assay Kit (
Ab39412
,
Abcam
); a Mouse TGF-β1 ELISA Kit (catalog
LS-F5184
-1,
LifeSpan BioSciences
); a
Mouse Albumin ELISA Kit
(15)
Exendin-4 Protects against Hyperglycemia-Induced Cardiomyocyte Pyroptosis via the AMPK-TXNIP Pathway
Journal of diabetes research
January 1, 2019
Wei H, Bu R, Yang Q, Jia J, Li T, Wang Q, Chen Y.
Activity
Assay
. Caspase-1 activity was determined using the Caspase-1 Assay Kit (fluorometric) from
Abcam
(
ab39412
, UK) according to the manufacturer’s instructions. Briefly, cells were lysed and the nuclei and
organelles
(16)
Involvement of inflammasome activation via elevation of uric acid level in nociception in a mouse model of muscle pain
Molecular pain
January 1, 2019
Yoshida S, Hagiwara Y, Tsuchiya M, Shinoda M, Koide M, Hatakeyama H, Chaweewannakorn C, Suzuki K, Yano T, Sogi Y,
et al
.
Uric Acid Colorimetric/Fluorometric Assay Kit
(
K608-100
,
BioVision
Inc., Milpitas, CA, USA) and Caspase 1 Assay Kit (
ab39412
,
Abcam plc
), respectively. Tissue samples were disrupted and homogenized using buffers from each kit.
(17)
CaMKIIδ-mediated inflammatory gene expression and inflammasome activation in cardiomyocytes initiate inflammation and induce fibrosis
JCI Insight
June 21, 2018
Andrew Willeford, Takeshi Suetomi, Audrey Nickle, Hal M. Hoffman, Shigeki Miyamoto, Joan Heller Brown
NRVMs using a prevalidated Fluorometric
Caspase-1
Activity Kit as described by the manufacturer (
Abcam
,
ab39412
).
Immunoblotting
.
Western blot analysis
was performed on snap-frozen ventricular tissue. Tissue samples were
(18)
Carnosic acid prevents dextran sulfate sodium-induced acute colitis associated with the regulation of the Keap1/Nrf2 pathway
Scientific Reports
December 1, 2017
Neng Yang, Zongling Xia, Naiyuan Shao, Bowen Li, Lian Xue, Ya Peng, Feng Zhi, Yilin Yang
centrifugation
at 10,000 g for 10 min. The activity of caspase-1 was determined by a commercial kit (
Abcam
,
ab39412
) according to the manufacturer’s instructions.
Immunofluorescence
histochemistry
For
immunofluorescence
(19)
Developmental Role of Macrophage Cannabinoid-1 Receptor Signaling in Type 2 Diabetes
Diabetes
April 1, 2017
Tony Jourdan et al.
Inflammation
Caspase-1
activity was determined using the
Caspase-1 Assay Kit (Fluorometric
) from
Abcam
(
ab39412
); interleukin (IL)-1b and
tumor
necrosis
factor-a (TNF-a) proteins were measured using the
Rat
Quantikine
(20)
PKM2-dependent glycolysis promotes NLRP3 and AIM2 inflammasome activation
Nature communications
October 25, 2016
Xie M, Yu Y, Kang R, Zhu S, Yang L, Zeng L, Sun X, Yang M, Billiar TR, Wang H,
et al
.
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Caspase-1 Assay Kit (Fluorometric) from Abcam
Supplier Page
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