Caspase-1 Assay Kit (Fluorometric) ab39412 from Abcam

Product Specs
ItemCaspase-1 Assay Kit (Fluorometric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab39412
Quantity100 Test
Sample TypeTissue Extracts, Cell Lysate
Assay Time2h 0m
ApplicationsEnzyme activity assay
Gene NameCASP1
NCBI Gene AliasesICE, IL1BC, P45
Research AreaImmunology & Infectious Disease
NCBI Full Gene Namecaspase 1

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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Human resistin is critical to activation of the NLRP3 inflammasome in macrophagesPLoS OneApril 10, 2026Udeshika Kariyawasam, Winson Lam, John Skinner, Rituparna Chakrabarti, Andrea Cox, Paul M. Hassoun, Qing Lin, Roger A. Johnsdetermined by detecting the cleavage of YVAD-AFC using the Abcam Caspase-1 Assay Kit (Fluorometric, ab39412). Briefly, hResistin-treated THP-1–converted macrophages were treated with (1) cell culture medium only,

(2) Role of the Two-Pore-Domain Potassium channel TREK-1 (K2P2.1) in Hyperoxia- and Mechanical Stretch-Induced Alveolar Epithelial InjuryAmerican Journal of Physiology-Lung Cellular and Molecular PhysiologyJanuary 1, 2026Nairrita Majumder, Benjamin Lopez, Aren Kasparian, John Taylor, Kushal Chatterjee, Hannah Zhong, Maya Fabrizio, Marina Angelini, Tatiana Zyrianova, Riccardo Olcese, et al.interleukin-1 converting enzyme (ICE) activity with a commercial fluorometric assay kit (Abcam, catalog #ab39412) as per manufacturer’s instructions. Briefly, cell lysates were used to measure the amount of AFC (AFC=7-Amino-4-trifluoromethylcoumarin)

(3) Celecoxib inhibits NLRP1 inflammasome pathway in MDA-MB-231 CellsNaunyn-Schmiedeberg's Archives of PharmacologyNovember 1, 2024Ege Arzuk, Derviş Birim, Güliz ArmağanIL-1β ELISA kit (RAB0273) were acquired from Sigma-Aldrich (St. Louis, MO), and caspase-1 activity kit (ab39412) was purchased from Abcam (MA, USA). Celecoxib and nimesulide were kindly provided by Deva Pharmaceutical

(4) Systemic immune challenge exacerbates neurodegeneration in a model of neurological lysosomal diseaseEMBO Molecular MedicineJune 18, 2024Oriana Mandolfo, Helen Parker, Èlia Aguado, Yuko Ishikawa Learmonth, Ai Yin Liao, Claire O’Leary, Stuart Ellison, Gabriella Forte, Jessica Taylor, Shaun Wood, et al.analysis. Caspase-1 activity assay Caspase-1 activity was measured as per the manufacturer’s protocol (ab39412; Abcam), using recombinant mouse caspase-1 (0.02-2U; ab52079; Abcam) standards. Plates were incubated

(5) Adipocyte pyroptosis occurs in omental tumor microenvironment and is associated with chemoresistance of ovarian cancerJournal of Biomedical ScienceJune 11, 2024Chang-Ni Lin, Yu-Ling Liang, Hsing-Fen Tsai, Pei-Ying Wu, Lan-Yin Huang, Yu-Han Lin, Chieh-Yi Kang, Chao-Ling Yao, Meng-Ru Shen, Keng-Fu Hsuand quantitation. Caspase-1 activity assay Caspase-1 activity measurement was by a commercial kit (ab39412; Abcam) which is based on the cleavage of the substrate YVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin)

(6) Isthmin-1 Improves Aging-Related Cardiac Dysfunction in Mice through Enhancing Glycolysis and SIRT1 Deacetylase ActivityAging and diseaseJanuary 25, 2024Min Hu, Xin Zhang, Yi-Peng Gao, Yu-Xin Hu, Teng Teng, Sha-Sha Wang, Qi-Zhu TangThermo Fisher Scientific (Waltham, MA, USA). SIRT1 Activity Assay Kit (ab156065) and Caspase-1 Assay Kit (ab39412) were purchased from Abcam (Cambridge, UK). lucigenin (#M8010), glucosamine hydrochloride (GlcN, #G4875),

(7) Polyphenol-rich diet mediates interplay between macrophage-neutrophil and gut microbiota to alleviate intestinal inflammationCell Death & DiseaseOctober 9, 2023Dandan Han, Yujun Wu, Dongdong Lu, Jiaman Pang, Jie Hu, Xiangyu Zhang, Zhenyu Wang, Guolong Zhang, Junjun WangCaspase-1 and NLRP3 activation in colonic and cellular lysis was measured using Caspase-1 Assay Kit (ab39412, Abcam, Cambridge, MA, USA) and NLRP3 ELISA Kit (ab279417, Abcam, Cambridge, MA, USA), respectively,

(8) Enhanced Activity of NLRP3 Inflammasome in the Lung of Patients with Anti-Synthetase SyndromeCellsDecember 23, 2022Espiridión Ramos-Martinez, Angel E. Vega-Sánchez, Gloria Pérez-Rubio, Mayra Mejia, Ivette Buendía-Roldán, Montserrat I. González-Pérez, Heidegger N. Mateos-Toledo, Warrison A. Andrade, Ramcés Falfán-Valencia, Jorge Rojas-SerranoAccording to the manufacturer’s instructions, the activity was detected using a Caspase-1 assay kit (ab39412, Abcam™, Cambridge, UK). All assays were performed in triplicate in three independent experiments. 2.7.

(9) Synthetic Secoisolariciresinol Diglucoside (LGM2605) Prevents Asbestos-Induced Inflammation and Genotoxic Cell Damage in Human Mesothelial CellsInternational Journal of Molecular SciencesSeptember 3, 2022Ralph A. Pietrofesa, Shampa Chatterjee, Yuwaraj Kadariya, Joseph R. Testa, Steven M. Albelda, Melpo Christofidou-Solomidouactivity was determined using a commercially available fluorometric assay kit (Caspase-1 Assay Kit, ab39412, Abcam, Waltham, MA, USA), as previously performed [15]. Briefly, the assay kit measures caspase-1 activity

(10) Complanatuside alleviates inflammatory cell damage induced by pro-inflammatory cytokines in skin keratinocytesFrontiers in ChemistryAugust 10, 2022Xiao Wang et al.fluorescence microscope. For the caspase-1 activity assay, the Caspase-1 assay kit (Fluorometric) (#ab39412, Abcam) was used. In brief, cell lysates were prepared after treatment. Reaction buffer and YVAD-AFC

(11) Galacto-oligosaccharides alleviate lung inflammation by inhibiting NLRP3 inflammasome activation in vivo and in vitroJournal of advanced researchJuly 1, 2022Cai Y, Gilbert MS, Gerrits WJJ, Folkerts G, Braber S.assay Caspase-1 activity in PBECs and A549 cells was determined in 50 μL cell lysates using a commercial kit (ab39412, Abcam, Cambridge, UK) according to the manufacturer’s instructions. For the in vivo experiments, same

(12) MicroRNA-1224-5p Aggravates Sepsis-Related Acute Lung Injury in MiceOxidative Medicine and Cellular LongevityJune 28, 2022Bing Liu, Feng Chen, Ni-Tao Cheng, Zheng Tang, Xian-Guo Wang, Ming Xu(ab102526), myeloperoxidase (MPO) activity assay kit (ab105136), and caspase-1 activity assay kit (ab39412) were all purchased from Abcam (Cambridge, MA, USA). TransAM® NF-κB p65 kit (40097) was purchased from

(13) Role of Polyphenol-Derived Phenolic Acid in Mitigation of Inflammasome-Mediated Anxiety and DepressionBiomedicinesMay 28, 2022Ruth Iban-Arias, Maria Sebastian-Valverde, Henry Wu, Weiting Lyu, Qingli Wu, Jim Simon, Giulio Maria Pasinetticaspase-1 (Cat#ab39901, Abcam, Cambridge, UK), and the fluorometric Caspase-1 Assay Kit (Abcam, Cat#ab39412, Abcam, Cambridge, UK), by following the protocol established by the fabricator. 2.1.9. THP1 Macrophages THP1

(14) RGS6 Drives Spinal Cord Injury by Inhibiting AMPK Pathway in MiceDisease markersJanuary 1, 2022Dao W, Xiao Z, Yang W, Luo X, Xia H, Lu Z.and tumor necrosis factor-alpha (TNF-α, ab208348) ELISA kits, and fluorometric caspase-1 assay kit (ab39412) were purchased from Abcam (Cambridge, MA, USA). Lentiviral vectors carrying short hairpin RNA against

(15) TRPV1 Hyperfunction Contributes to Renal Inflammation in Oxalate NephropathyInternational Journal of Molecular SciencesJune 8, 2021Chien-Lin Lu, Te-Yi Teng, Min-Tser Liao, Ming-Chieh Main cell lysates and renal cortical tissues was determined using a commercial fluorometric assay kit (ab39412) obtained from Abcam (Bristol, UK) and expressed as units/mg of protein. 4.16. Statistics Numerical

(16) Deubiquitination of NLRP6 inflammasome by Cyld critically regulates intestinal inflammationNature immunologyJune 1, 2020Mukherjee S, Kumar R, Tsakem Lenou E, Basrur V, Kontoyiannis DL, Ioakeimidis F, Mosialos G, Theiss AL, Flavell RA, Venuprasad K.activity assay. The activity of caspase-1 was determined in colon homogenates by a commercial kit (Abcam, ab39412) according to the manufacturer’s instructions. Briefly, fresh frozen sections of colons were homogenized

(17) Haematopoietic stem cell gene therapy with IL-1Ra rescues cognitive loss in mucopolysaccharidosis IIIAEMBO molecular medicineMarch 6, 2020Parker H, Ellison SM, Holley RJ, O'Leary C, Liao A, Asadi J, Glover E, Ghosh A, Jones S, Wilkinson FL, et al.1 activity was measured using 100 μg total brain protein with the Caspase 1 Fluorometric Assay Kit (ab39412) as per the manufacturer's protocol (Abcam, UK), using recombinant mouse caspase 1 (0.02‐2U; Abcam)

(18) Tojapride prevents CaSR-mediated NLRP3 inflammasome activation in oesophageal epithelium irritated by acidic bile saltsJournal of cellular and molecular medicineJanuary 1, 2020Yin XL, Wu HM, Zhang BH, Zhu NW, Chen T, Ma XX, Zhang LY, Lv L, Zhang M, Wang FY, et al.Measurement of caspase‐1 activity The activity of caspase‐1 was detected using a Caspase 1 Assay kit (ab39412, Abcam) per the manufacturer's instructions. All assays were performed in triplicate in three independent

(19) The pore-forming subunit Kir6.1 of the K-ATP channel negatively regulates the NLRP3 inflammasome to control insulin resistance by interacting with NLRP3Experimental & molecular medicineAugust 6, 2019Du RH, Lu M, Wang C, Ding JH, Wu G, Hu G.pcDNA3-N-Flag-NLRP3 (75127) was obtained from Addgene (Watertown, MA, USA). A caspase-1 activity assay kit (ab39412) was obtained from Abcam (Cambridge, UK). All other materials were obtained as described elsewhere22. Mice Kir6.1

(20) Immunoglobulin light chains generate proinflammatory and profibrotic kidney injuryThe Journal of clinical investigationJune 17, 2019Ying WZ, Li X, Rangarajan S, Feng W, Curtis LM, Sanders PW.Millipore Sigma); a Caspase-1 Colorimetric Assay Kit (Ab39470, Abcam) or a Caspase-1 Fluorometric Assay Kit (Ab39412, Abcam); a Mouse TGF-β1 ELISA Kit (catalog LS-F5184-1, LifeSpan BioSciences); a Mouse Albumin ELISA Kit

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Caspase-1 Assay Kit (Fluorometric) from Abcam

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Caspase-1 Assay Kit (Fluorometric) from Abcam

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