Fig 1: A. indica affected the proinflammatory cytokines production and NF-?B transcription factors protein expression.Protein expression levels of (A) IL-1 ß (B) TNF a (C) IKK a and ß and (D) NF-?B p65 were determined by ELISA. Values are statistically significant at p*<0.05, p**< 0.01, p***< 0.001compared with the control group while p#<0.05, p##< 0.01, p###< 0.001 compared with stress group. The experiment was carried out in triplicates (n = 3).
Fig 2: P. gingivalis supernatant impairs the osteogenic potential of PDLSC.(A) IL-1 ß, IL-6 and IL-8 production was measured using ELISA after PDLSCs were treated with P. gingivalis (P.g.) supernatant’s dilutions (1:500 and 1:50), or untreated cells as control simultaneously for 24 h. IL-1 ß, IL-6 and IL-8 is expressed in pg/ml ( ± standard deviation). (B) Western blot analysis showed the protein expression of TLR2, TLR4 and GAPDH was used as the internal control. (C) Confluent PDLSC were stained with Alizarin Red after 21 days of cultivation in osteogenic medium containing P. gingivalis supernatant’s dilutions (1:500 and 1:50) and Alizarin Red was then extracted and measured for light absorbance at 405 nm. *p < 0.05 as determined by unpaired two-tailed t tests. Data from three biologically independent replicates.
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