Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit ab109882 from Abcam

Product Specs
ItemPyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab109882
MethodFuncS
Quantity30 Test
ApplicationsFuncS

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) A therapeutic approach to pantothenate kinase associated neurodegeneration: a pilot studyOrphanet Journal of Rare DiseasesNovember 28, 2024Alessandra Pereira, Carolina Fischinger Moura de Souza, Mónica Álvarez-Córdoba, Diana Reche-López, José Antonio Sánchez-Alcázarwhole cells was measured using the Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit (ab109882, ABCAM, Cambridge, MA, USA) according to manufacturer’s instructions. Three biological replicates were

(2) Alpha-lipoic acid supplementation corrects pathological alterations in cellular models of pantothenate kinase-associated neurodegeneration with residual PANK2 expression levelsOrphanet Journal of Rare DiseasesApril 12, 2023Marta Talaverón-Rey, Mónica Álvarez-Córdoba, Irene Villalón-García, Suleva Povea-Cabello, Juan M. Suárez-Rivero, David Gómez-Fernández, Ana Romero-González, Alejandra Suárez-Carrillo, Manuel Munuera-Cabeza, Paula Cilleros-Holgado, et al.whole cells was measured using the Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit (ab109882, ABCAM, Cambridge, MA, USA) according to manufacturer’s instructions. Three biological replicates were

(3) Therapeutic approach with commercial supplements for pantothenate kinase-associated neurodegeneration with residual PANK2 expression levelsOrphanet Journal of Rare DiseasesAugust 9, 2022Mónica Álvarez-Córdoba et al.whole cells was measured using the Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit (ab109882, ABCAM, Cambridge, MA, USA) according to manufacturer’s instructions. Three biological replicates were

(4) Down regulation of the expression of mitochondrial phosphopantetheinyl-proteins in pantothenate kinase-associated neurodegeneration: pathophysiological consequences and therapeutic perspectivesOrphanet Journal of Rare DiseasesDecember 1, 2021Mónica Álvarez-Córdoba, Marta Talaverón-Rey, Irene Villalón-García, Suleva Povea-Cabello, Juan M. Suárez-Rivero, Alejandra Suárez-Carrillo, Manuel Munuera-Cabeza, Joaquín J. Salas, José A. Sánchez-Alcázarwhole cells was measured using the Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit (ab109882, ABCAM, Cambridge, MA, USA) according to manufacturer’s instructions. Three biological replicates were

(5) Repurposing dichloroacetate for the treatment of women with endometriosisProceedings of the National Academy of Sciences of the United States of AmericaDecember 17, 2019Horne AW, Ahmad SF, Carter R, Simitsidellis I, Greaves E, Hogg C, Morton NM, Saunders PTK.centrifugal analyzer (Roche Diagnostics). PDH enzyme activity was measured using a Dipstick assay kit (ab109882; Abcam): band intensity was analyzed using ImageJ. “Lesion in a Dish” Coculture System. Endo HPMCs

(6) Transcriptomic Signature of Right Ventricular Failure in Experimental Pulmonary Arterial Hypertension: Deep Sequencing Demonstrates Mitochondrial, Fibrotic, Inflammatory and Angiogenic AbnormalitiesInternational Journal of Molecular SciencesSeptember 12, 2018Francois Potus, Charles Colin Thomas Hindmarch, Kimberly J. Dunham-Snary, Jeff Stafford, Stephen L. Archer

(7) Biventricular Increases in Mitochondrial Fission Mediator (MiD51) and Proglycolytic Pyruvate Kinase (PKM2) Isoform in Experimental Group 2 Pulmonary Hypertension-Novel Mitochondrial AbnormalitiesFrontiers in cardiovascular medicineJanuary 1, 2018Xiong PY, Tian L, Dunham-Snary KJ, Chen KH, Mewburn JD, Neuber-Hess M, Martin A, Dasgupta A, Potus F, Archer SL.were then applied to PDH enzyme activity dipstick assay following the manufacturer’s protocol (Cat# ab109882, Abcam, Cambridge MA, USA), as previously described (20). Statistical Analysis Values are expressed

(8) Nox4 reprograms cardiac substrate metabolism via protein O-GlcNAcylation to enhance stress adaptationJCI InsightDecember 21, 2017Adam A. Nabeebaccus et al.enzymatic activity was measured in mitochondrial fractions using a commercially available dipstick assay (ab109882, Abcam) and normalized to the relative amount of PDH E1α protein. GAPDH activity was measured in heart

(9) HIF-1α is required for disturbed flow-induced metabolic reprogramming in human and porcine vascular endotheliumeLifeMay 30, 2017Wu D, Huang RT, Hamanaka RB, Krause M, Oh MJ, Kuo CH, Nigdelioglu R, Meliton AY, Witt L, Dai G, et al.milk and TBST, Cell Signaling, 7074S, RRID:AB_2099233). Pyruvate dehydrogenase (PDH) activity (Abcam, ab109882) HAECs were plated at 4 × 105 cells/well in a 6-well plate overnight before either UF or DF flow for

(10) MicroRNA-138 and MicroRNA-25 Down-regulate Mitochondrial Calcium Uniporter, Causing the Pulmonary Arterial Hypertension Cancer PhenotypeAmerican Journal of Respiratory and Critical Care MedicineFebruary 15, 2017Zhigang Hong et al.which the intensity of the nitroblue tetrazolium band (arrows) is proportionate to enzyme activity (ab109882; MitoSciences-Abcam, Eugene, OR). (K) Lactate production is increased in normal PASMCs by inhibiting

(11) E4F1 controls a transcriptional program essential for pyruvate dehydrogenase activityProceedings of the National Academy of Sciences of the United States of AmericaSeptember 27, 2016Lacroix M, Rodier G, Kirsh O, Houles T, Delpech H, Seyran B, Gayte L, Casas F, Pessemesse L, Heuillet M, et al.extracts prepared from cells or muscles (gastrocnemius and heart). PDH activity DipStick Assay Kit (ab109882, Abcam) was used on cell (25 μg) or muscle (5 μg) extracts prepared according to the manufacturer’s

(12) Total body exposure to low-dose ionizing radiation induces long-term alterations to the liver proteome of neonatally exposed miceJournal of proteome researchJanuary 2, 2015Bakshi MV, Azimzadeh O, Barjaktarovic Z, Kempf SJ, Merl-Pham J, Hauck SM, Buratovic S, Eriksson P, Atkinson MJ, Tapio S.treated liver samples from PND 11. The assay was performed following manufacturer’s guidelines (Abcam # ab109882). The band intensities were quantified by using the total lab (TL) 100 software. PPAR alpha transcription

(13) Role of Dynamin-Related Protein 1 (Drp1)-Mediated Mitochondrial Fission in Oxygen Sensing and Constriction of the Ductus ArteriosusCirculation ResearchMarch 1, 2013Zhigang Hong et al.PDH Enzyme Activity Dipstick Assay Kit following the manufacturer’s instruction (MitoSciences-Abcam, ab109882, Eugene Oregon). This technique uses anti-PDH antibody immobilized on a capture line section of the

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Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit from Abcam

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Pyruvate dehydrogenase (PDH) Enzyme Activity Dipstick Assay Kit from Abcam

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