Mouse Interferon gamma ELISPOT Kit (with un-coated plates) ab46587 from Abcam

Product Specs
ItemMouse Interferon gamma ELISPOT Kit (with un-coated plates)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab46587
Quantity5 x 96 Tests, 10 x 96 Tests
Gene NameIFNG
NCBI Gene AliasesIFN-g, If2f, Ifg
ApplicationsELISPOT
Research AreaImmunology & Infectious Disease
NCBI Full Gene Nameinterferon gamma
MethodELISPOT

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Reducing cell intrinsic immunity to mRNA vaccine alters adaptive immune responses in miceMolecular therapy. Nucleic acidsDecember 12, 2023Wang Z, Jacobus EJ, Stirling DC, Krumm S, Flight KE, Cunliffe RF, Mottl J, Singh C, Mosscrop LG, Santiago LA, et al.T cell ELISpot IFN-γ ELISpot assays were performed on mouse splenocytes using a commercial kit (cat.no. ab64029, AbCam) following the manufacturer’s recommendations. Cells were stimulated with 2 μg/mL anti-CD28 (Clone

(2) An mRNA-based T-cell-inducing antigen strengthens COVID-19 vaccine against SARS-CoV-2 variantsNature communicationsMay 23, 2023Tai W, Feng S, Chai B, Lu S, Zhao G, Chen D, Yu W, Ren L, Shi H, Lu J, et al.potency. ELISpot assays of IFN-γ and IL-4 Mice ELISpot assays were performed with mouse IFN-γ (Abcam, Cat. No# ab64029) and IL-4 ELISpot kits (Mabtech, Cat. No# 3311-4HPW-10) according to the manufacturer’s instructions.

(3) Adaptation of transgene mRNA translation boosts the anticancer efficacy of oncolytic HSV1Journal for ImmunoTherapy of CancerMarch 23, 2023Huy-Dung Hoang, Aida Said, Nasana Vaidya, Victoria H Gilchrist, Kyle Malone, Usha Kabilan, Serena Topshee, Xiao Xiang, An-Dao Yang, David Olagnier, et al.penicillin/streptomycin (Fisher). IFNγ ELISPOT was done using the mouse interferon-gamma ELISPOT kit (Abcam, CAT# ab64029) according to the manufacturer’s protocol. Briefly, 100,000 splenocytes were co-cultured with/without

(4) Formulation, inflammation, and RNA sensing impact the immunogenicity of self-amplifying RNA vaccinesMolecular therapy. Nucleic acidsMarch 14, 2023Tregoning JS, Stirling DC, Wang Z, Flight KE, Brown JC, Blakney AK, McKay PF, Cunliffe RF, Murugaiah V, Fox CB, et al.exclusion. T cell ELISPOT IFN-γ ELISPOT assays were performed using a commercial kit from AbCam (ab64029) following the manufacturer’s recommendations. Cells were stimulated with 2 μg/mL anti-CD28 (Clone 37.51:

(5) Exosomes mediate Coxsackievirus B3 transmission and expand the viral tropismPLoS pathogensJanuary 1, 2023Fu Y, Xiong S.determined. ELISpot SARS-CoV-2-specific cellular immune responses in ferrets were assessed by a Murine IFN-γ ELISpot Kit (#ab64029, Abcam) following the manufacturer’s instructions. In brief, 1×105 of peripheral blood mononuclear cells

(6) Lipid nanoparticle-mediated lymph node-targeting delivery of mRNA cancer vaccine elicits robust CD8(+) T cell responseProceedings of the National Academy of Sciences of the United States of AmericaAugust 23, 2022Chen J, Ye Z, Huang C, Qiu M, Song D, Li Y, Xu Q.10% fetal bovine serum. The ELISpot assay was conducted using the Mouse Interferon gamma ELISPOT Kit (ab64029, Abcam, USA). Then 2 × 104 PBMCs cells were incubated in complete RPMI-1640 medium with or without 2

(7) FAP-Targeted Photodynamic Therapy Mediated by Ferritin Nanoparticles Elicits an Immune Response against Cancer Cells and Cancer Associated FibroblastsAdvanced functional materialsFebruary 10, 2021Zhou S, Zhen Z, Paschall AV, Xue L, Yang X, Bebin-Blackwell AG, Cao Z, Zhang W, Wang M, Teng Y, et al.producing cells in splenocytes was measured using a mouse interferon gamma ELISpot Kit (Abcam, Cat. No. ab64029) following vendor’s protocol. Splenocytes freshly isolated from animals 12 days post tumor inoculation

(8) Detecting Tumor Antigen-Specific T Cells via Interaction-Dependent Fucosyl-BiotinylationCellNovember 12, 2020Liu Z, Li JP, Chen M, Wu M, Shi Y, Li W, Teijaro JR, Wu P.ELISpot assay for TIL reactivity analysis ELISpot assays were performed using a mouse IFN-γ ELISpot kit (ab64029, Abcam). DCs were primed with indicated antigens or tumor lysates as described above. Different subsets

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Mouse Interferon gamma ELISPOT Kit (with un-coated plates) from Abcam

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Mouse Interferon gamma ELISPOT Kit (with un-coated plates) from Abcam

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