Calcium Flux Assay Kit (Fluo-8, No Wash) ab112129 from Abcam

Product Specs
ItemCalcium Flux Assay Kit (Fluo-8, No Wash)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab112129
Quantity10 Kit, 96 Test
MethodFuncS
Sample TypeAdherent cells, Suspension cells
Assay Time1h 0m

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(1) Enterovirus-induced cleavage of Mitofusin 2 generates mitophagosomes for enveloped virion releaseSci AdvApril 24, 2026Alagie Jassey, Bimal Paudel, Michael A. Wagner, Noah Pollack, I-Ting Cheng, Raquel Godoy-Ruiz, David J. Weber, William T. Jacksonmonomeric, green fluorescent form. For cellular calcium assay, the Fluo-8 No Wash Calcium Assay Kit (Abcam, ab112129) was used following the manufacturer’s instructions. In this assay, cells are incubated with Fluo-8.

(2) PRRSV NSP5 orchestrates dual immune disruption by targeting NLRP3 and STINGVet ResOctober 16, 2025Xiangyu Huang, Xuyan Xiang, Xiaohan Jiang, Weiyu Qu, Yufei Zhang, Zhenchao Zhao, Minjie Li, Haiwei Wang, Xin Lidetection kit (170–5061, BIO-RAD). Ca2+ measurements The Fluo-8 Calcium Flux Analysis Kit (Abcam, ab112129) was used to measure cytoplasmic Ca2+ levels. Briefly, at the specified time points, 100 μL of Fluo-8

(3) Congenital Hypothyroidism Dysregulates TRPC6 to Mediate Abnormal Dendritic Spine Growth of Hippocampal NeuronsCNS Neuroscience & TherapeuticsSeptember 25, 2025Tianci Li, Feifei Shen, Lingling Li, Peng Chen, Zhiwen Zhu, Yuqin Zheng, Haiying Li, Guihai Suo, Yongjun Wang, Jinlong Shi, et al.experiment was performed according to the procedure of Fluo‐8 Calcium Flux Assay Kit—No Wash (Abcam, Cat# ab112129). All kit components were thawed at room temperature before use, and the Fluo‐8 stock solution was prepared.

(4) Protocol for live-cell calcium imaging of human lung microvascular endothelial vessel-on-a-chip modelSTAR ProtocolsSeptember 19, 2025Nethika Ariyasinghe, Jean-Pierre Amoakon, Anjaparavanda P. Narenassays,Critical commercial assays,Critical commercial assays ,, Fluo-8 Calcium Flux Assay Kit - No Wash,Abcam,ab112129 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, Human

(5) Lactobacillus rhamnosus-derived extracellular vesicles influence calcium deposition in a model of breast cancer intraductal calcium stressiScienceJune 20, 2025Ngoc Vuong, Melany Alomia, Ahana Byne, Purva Gade, Thomas Raymond Philipson, Rayan Ibrahim Alhammad, Cade J. Skislak, Intisar Alruwaili, Fahad M. Alsaab, Weidong Zhou, et al.assays,Critical commercial assays,Critical commercial assays ,, Fluo-8 Calcium Flux Assay Kit,Abcam,ab112129 ,, Deposited data,Deposited data,Deposited data ,, Proteomics data,MASSive/ProteomeXchange,PXD061715Calcium

(6) Identification of a molecular resistor that controls UCP1-independent Ca2+ cycling thermogenesis in adipose tissueCell MetabolismApril 7, 2025Christopher Auger, Mark Li, Masanori Fujimoto, Kenji Ikeda, Jin-Seon Yook, Timothy R. O’Leary, María Paula Huertas Caycedo, Cai Xiaohan, Satoshi Oikawa, Anthony R.P. Verkerke, et al.above-mentioned protocol. Intracellular Ca2+ levels were determined using the Fluo-8 No Wash Calcium Assay kit (ab112129; Abcam). Differentiated adipocytes were incubated with Fluo-8 in calcium-free Hanks’ balanced salt solution

(7) Dolutegravir induces endoplasmic reticulum stress at the blood-brain barrierFASEB journal : official publication of the Federation of American Societies for Experimental BiologyFebruary 28, 2025Huang C, Qu QR, Hoque MT, Bendayan R.polyclonal anti‐cytochrome c (ab110325), anti‐VDAC1 (ab14734) antibodies, Fluo‐8 Calcium Flux Assay Kit (ab112129), and Mitochondria/Cytosol Fractionation Kit (ab65320) were purchased from Abcam (Boston, MA, USA).

(8) TRPV4 modulates inflammatory responses and apoptosis in enteric glial cells triggered by Clostridioides difficile toxins A and BJ Inflamm (Lond)January 14, 2025Dvison de Melo Pacífico, Deiziane Viana da Silva Costa, Maria Lucianny Lima Barbosa, Conceição Silva Martins Rebouças, Simone de Goes Simonato, Cirle Alcantara Warren, Maria Luana Gaudencio Dos Santos Morais, Renata Ferreira de Carvalho Leitao, Gerly Anne de Castro Britoplates. The intracellular Ca²⁺ levels were measured using the Fluo-8 no wash calcium assay kit (Abcam, ab112129) following the manufacturer’s instructions. In brief, 100 µL of Fluo-8 dye-loading solution was added

(9) Mechanistic insights into the selective targeting of P2X3 receptor by camlipixant antagonistJournal of Biological ChemistryDecember 18, 2024Trung Thach, KanagaVijayan Dhanabalan, Prajwal Prabhakarrao Nandekar, Seth Stauffer, Iring Heisler, Sarah Alvarado, Jonathan Snyder, Ramaswamy Subramanianinflux assay The assay was performed using the Fluo-8 Calcium Flux Assay Kit (Abcam; catalog no.: ab112129), following the manufacturer's instructions. Cells were centrifuged from the culture medium and resuspended

(10) Mitophagosomes induced during EV-D68 infection promote viral nonlytic releasebioRxivDecember 10, 2024Alagie Jassey, Bimal Paudel, Michael A. Wagner, Noah Pollack, I-Ting Cheng, Raquel Godoy-Ruiz, David J. Weber, William T. Jacksonmanufacturer’s instructions. For cellular calcium assay, Fluo-8 Calcium Assay Kit – No Wash (Abcam, ab112129) was used following the manufacturer’s instructions. In-vitro cleavage assay Pierce™ HRV 3C Protease

(11) A Second Drug Binding Site in P2X3bioRxivNovember 21, 2024Trung Thach, KanagaVijayan Dhanabalan, Prajwal Prabhakarrao Nandekar, Seth Stauffer, Iring Heisler, Sarah Alvarado, Jonathan Snyder, Ramaswamy Subramaniancalcium influx assay The assay was performed using the Fluo-8 Calcium Flux Assay Kit (Abcam, Cat# ab112129), following the manufacturer's instructions. Cells were centrifuged from the culture medium and resuspended

(12) Secreted Apoe rewires melanoma cell state vulnerability to ferroptosisScience advancesOctober 18, 2024More S, Bonnereau J, Wouters D, Spotbeen X, Karras P, Rizzollo F, Killian T, Venken T, Naulaerts S, Vervoort E, et al.concentration. Calcium assay Intracellular calcium in the cells was analyzed by using Fluo-8 Calcium Flux Assay Kit (#ab112129) from Abcam. The cells were seeded in black 96-well plates, and after treatment with rhApoE3 for 3 days,

(13) GATA-4 overexpressing BMSC-derived exosomes suppress H/R-induced cardiomyocyte ferroptosisiScienceOctober 18, 2024Zhiyuan Xiao, Si Li, Xinxin Wu, Xinhao Chen, Dan Yan, Jigang HeCa2+ and H2S The Ferrous Ion Content Assay Kit (BC5415, Solarbio), Fluo-8 calcium flux assay kit (ab112129, Abcam), and H2S content Assay Kit (BC2055, Solarbio), were used to measure the concentration of Fe2+,

(14) Disruption of KLHL6 Fuels Oncogenic Antigen Receptor Signaling in B-Cell LymphomaBlood Cancer DiscoverySeptember 3, 2024Leo Meriranta, Selma Sorri, Kanutte Huse, Xiaonan Liu, Ivana Spasevska, Sadia Zafar, Iftekhar Chowdhury, Olli Dufva, Eerika Sahlberg, Luka Tandarić, et al.the signal of the control treatment. Calcium Release Assay Fluo-8 Calcium Flux Assay Kit (Abcam, ab112129) was used according to manufacturer’s instructions to assess kinetics of BCR cross-linking induced Ca2+

(15) An increase in ER stress and unfolded protein response in iPSCs-derived neuronal cells from neuronopathic Gaucher disease patientsScientific reportsApril 22, 2024Pornsukjantra T, Saikachain N, Sutjarit N, Khongkrapan A, Tubsuwan A, Bhukhai K, Tim-Aroon T, Anurathapan U, Hongeng S, Asavapanumas N.Subsequently, the cells were incubated with a calcium indicator dye (Fluo-8 Calcium Flux Assay Kit, ab112129, Abcam, Cambridge, UK) for 1 h. After the incubation with the dye, the cells were kept in Hanks’ Balanced

(16) Self-organization of vascularized skeletal muscle from bovine embryonic stem cellsbioRxivMarch 28, 2024Marina Sanaki-Matsumiya, Casandra Villava, Luca Rappez, Kristina Haase, Jun Wu, Miki EbisuyapyBOAT67. Calcium oscillation measurement Calcium activity was visualized with Fluo-8 AM (Abcam, ab112129) following the manufacturer’s protocol. Briefly, cells were washed twice with PBS(−), and incubated

(17) An orexin-receptor-2-mediated heart-brain axis in cardiac painiScienceMarch 15, 2024Han Jiao, Yongjin Wang, Kang Fu, Xiaoao Xiao, Mo-Qiu Jia, Jia Sun, Jingxiao Wang, Guoqing Zhu, Daying Lyu, Qiulun Lu, et al.primary culture section. Intracellular Ca2+ flux was determined by Fluo-8 No Wash Calcium Assay kit (ab112129, Abcam). Cells were incubated with Fluo-8 for 30 min at 37°C in calcium-free Hanks' balanced salt solution

(18) Forchlorfenuron-Induced Mitochondrial Respiration Inhibition and Metabolic Shifts in Endometrial CancerCancers (Basel)February 28, 2024Kyukwang Kim, Negar Khazan, Rachael B. Rowswell-Turner, Rakesh K. Singh, Taylor Moore, Myla S. Strawderman, John P. Miller, Cameron W. A. Snyder, Ahmad Awada, Richard G. Mooreplate was incubated according to the manufacturer’s protocol (Fluo-8 No Wash Calcium Assay, Abcam, ab112129). Once the vehicle, FCF, or thapsigargin were added to appropriate wells, fluorescence intensity was

(19) Na(v)1.7 as a chondrocyte regulator and therapeutic target for osteoarthritisNatureJanuary 1, 2024Fu W, Vasylyev D, Bi Y, Zhang M, Sun G, Khleborodova A, Huang G, Zhao L, Zhou R, Li Y, et al.chamber (Thermo Fisher, 154461) and loaded with 5 µM CoroNa Green (Invitrogen, C36676) or Fluo-8 (Abcam, ab112129) in Hanks’ Balanced Salt Solution for 45 min at 37 °C in the presence or absence of 25 nM ProTx II or

(20) Oleic acid availability impacts thymocyte preprogramming and subsequent peripheral Treg cell differentiationNature ImmunologyJanuary 1, 2024Liangyu Lin, Mingyuan Hu, Qing Li, Liming Du, Li Lin, Yueqing Xue, Fanjun Zheng, Fei Wang, Keli Liu, Yu Wang, et al.14–0451-82). These stained cells were then mixed at a 1:1 ratio and loaded with Fluo-8 dye (Abcam, cat no. ab112129) according to the manufacturer’s instructions. The fluo-8 loaded cells were washed with calcium-free

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Calcium Flux Assay Kit (Fluo-8, No Wash) from Abcam

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Calcium Flux Assay Kit (Fluo-8, No Wash) from Abcam

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