Fig 1: Relative mRNA expression levels of Ctgf and Acta2 in cardiac fibroblasts exposed to recombinant BNP and/or TGFß1 after 4 h (a) and 24 h (b) (n = 8) * p < 0.05; ** p < 0.01. Bar indicates mean.
Fig 2: Evaluation of the PPHN model using different parameters. (A) Medial wall thickness percentage (WT %) of pulmonary arteries between 50 and 100 µm in diameter. Medial wall thickness (%) = (2 × medial wall thickness)/external diameter. (B) Medial wall area percentage (WA %) from pulmonary arteries between 50 and 100 µm in diameter. Medial wall area (%) = medial wall area/total area. (C) The ratio of RV/(LV + SV). (D) Plasma BNP concentration. Data are represented as the mean ± standard deviation; n=10/group; **P<0.01 vs. Control. BNP, B-type natriuretic peptide; LV, left ventricle; PPHN, persistent pulmonary hypertension of the newborn; RV, right ventricle; SV, ventricular septum.
Fig 3: Relative mRNA expression levels of Nppb (a) Myh7 (b) and Col1a1 (c) in cardiomyocytes (CM) (n = 6) and cardiac fibroblasts (CF) (n = 5) (presented on a logarithmic scale). Bar indicates mean.
Fig 4: Relative mRNA expression levels of Nppb, Tgfb1, Tnc, Ctgf, Acta2, and Piezo1 in cardiac fibroblasts after stimulation with Piezo1 agonist Yoda1 after 4 h (n = 6) (a) Relative mRNA expression of Nppb, Tgfb1, Tnc, Ctgf, Acta2, and Piezo1 in cardiac fibroblasts exposed to 10% 1 Hz cyclic stretch for 6 h (S) or non-stretched (NS) conditions after transfection with either control siRNA (Scrambled SI) or Piezo1-specific siRNA (Piezo1 SI) (n = 6) (b) * p < 0.05. Bar indicates mean.
Fig 5: Relative mRNA expression levels of Nppb, Tgfb1, Tnc, Ctgf, Acta2, and Piezo1 in cardiac fibroblasts exposed to 10% 1 Hz cyclic stretch (S) or non-stretched (NS) conditions for 4 h (n = 5–12) (a) or 24 h (n = 5–12) (b). BNP-protein concentration in conditioned media from stretched and non-stretched cardiac fibroblasts (n = 4) (c). * p < 0.05; ** p < 0.01; *** p < 0.001. Bar indicates mean.
Supplier Page from Abcam for Rat BNP 32 ELISA Kit