Fig 1: CMS stimulated Sele overexpression and Sele-dependent monocyte adhension to Endothelial cells. A Protein expression of Sele in primary human dermal vascular endothelial cells assessed in a tensile strength-dependent manner. B Quantitative analysis of Sele expression levels. C, D Representative fluorescence images and quantitative analysis of the monocyte adhesion to endothelial cells in a tensile strength-dependent manner. Scale bar = 200 μm. E High-resolution confocal microscopy image of co-cultured endothelial cells and monocyte. Scale bar = 50 μm. F Correlation analysis between Sele expression levels and monocyte adhesion numbers. G, H Volcano plot of the detection results of the multi-inflammation chip for the supernatant from static and stretched groups. I, J Representative fluorescence images and quantitative analysis of monocyte adhesion to HMEC-1 cells in control, stretched, and stretched with Sele knocking out groups. Scale bar = 200 μm. Release of IL-1a (K), TNF-a (L), and IL-6 (M) in co-cultures of HMEC-1 cells and monocytes (n = 3. *p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant)