Mouse Interferon gamma ELISPOT Kit ab64029 from Abcam

Product Specs
ItemMouse Interferon gamma ELISPOT Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab64029
MethodELISPOT
Quantity1 x 96 Tests, 5 x 96 Tests
Gene NameIFNG
NCBI Gene AliasesIFN-g, If2f, Ifg
Research AreaImmunology & Infectious Disease
NCBI Full Gene Nameinterferon gamma
ApplicationsELISPOT

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Robust CD8+ T cell responses induced by an mRNA-LNP vaccine encoding rat HER2 extracellular domain confer prophylactic tumor protectionFront ImmunolApril 1, 2026Kexin Li, Xiaoya Li, Cong Zhang, Suli Dai, Lei Li, Jun Wang, Hongtao Zhang, Lianmei Zhaohours at 37 °C. The production of IFN-γ was quantified using the Mouse Interferon-gamma ELISPOT Kit (#ab64029, Abcam) following the manufacturer’s protocol. After incubation, the plates were washed and sequentially

(2) Armored macrophage-targeted CAR-T cells reset and reprogram the tumor microenvironment and control metastatic cancer growthCancer CellMarch 9, 2026Jaime Mateus-Tique, Ashwitha Lakshmi, Bhavya Singh, Rhea Iyer, Alfonso R. Sánchez-Paulete, Chiara Falcomatà, Matthew Lin, Gvantsa Pantsulaia, Alexander Tepper, Trung Nguyen, et al.the cells, wash twice with PBS, and seed 4×104 irradiated ID8-BVluc in a 96-well ELISPOT well (Abcam #ab64029). We isolate CD8 T cells from treated mouse spleens as previously described. We seeded 8×104 CD8 T cells

(3) Modifying non-coding regions of linear DNA vaccines to explore the interplay of expression and inflammation in immunogenicityHuman Vaccines & ImmunotherapeuticsDecember 31, 2025David C. Stirling, Maria de Miguel Ferrer, Sungwon Kim, Madina Wane, Daniel Kysh, Lisa J. Caproni, John S. Tregoningstains. ELISpot of splenocytes ELISpot assays were conducted using a commercially available kit from Abcam (ab64029), according to the manufacturer’s guidelines. The cells were stimulated with 1.25 μg/mL of anti-CD28

(4) A One Health approach to bovine coronavirus vaccine development, using LSDV as a live virus vectorHuman vaccines & immunotherapeuticsDecember 1, 2025Chineka N, Whittle L, Douglass N, Williamson AL, Chapman R.enzyme-linked immunosorbent spot (ELISPOT) assay was performed using the murine ELISPOT kit (Abcam, ab64029) and cells were stimulated with 5 ug/ml of BCoV spike and nucleocapsid proteins as previously described

(5) A Rationally Engineered Spleen‐Tropic One‐Component Lipid‐mRNA Complex (OncoLRC) for Cancer VaccinesAdvanced ScienceNovember 21, 2025Qimeng Yin, Chenchen Zhang, Jiahao Li, Kun Huang, Min Qiu10% fetal bovine serum. The ELISpot assay was performed using the Mouse Interferon Gamma ELISPOT Kit (ab64029, Abcam, USA). A total of 1 × 10⁶ spleen cells were incubated in complete RPMI‐1640 medium, with or without

(6) Spleen-targeted NeoPol-mL242 mRNA vaccine induces robust T-cell responses in a hepatocellular carcinoma modelJournal of NanobiotechnologySeptember 2, 2025Yufei Wu, Gongrui Sun, Wendan Ren, Yang Gui, Cong Wang, Xinyi Ye, Yun Chen, Xiufeng Pang, Qi Zhang, Zi Jun Wang, et al.with 5 × 105 splenic T cells in 96-well Multiscreen plates pre-coated with anti-IFN-γ antibody (Abcam, ab64029). The co-culture was maintained at 37 °C with 5% CO2 for 48 h. IFN-γ spot-forming cells were visualized

(7) An mRNA vaccine against monkeypox virus inhibits infection by co-activation of humoral and cellular immune responsesNature communicationsMarch 26, 2025Tai W, Tian C, Shi H, Chai B, Yu X, Zhuang X, Dong P, Li M, Yin Q, Feng S, et al.reduction. ELISpot assays of IFN-γ and IL-4 ELISpot assays were conducted utilizing mouse IFN-γ (Abcam, Cat. No# ab64029) and IL-4 ELISpot kits (Mabtech, Cat. No# 3311-4HPW-10) following the manufacturer’s instructions. The

(8) Optimizing a linear ‘Doggybone’ DNA vaccine for influenza virus through the incorporation of DNA targeting sequences and neuraminidase antigenDiscovery ImmunologyJanuary 3, 2024Robert F Cunliffe, David C Stirling, Ilaria Razzano, Valarmathy Murugaiah, Emanuele Montomoli, Sungwon Kim, Madina Wane, Helen Horton, Lisa J Caproni, John S Tregoningmean signal. T cell ELISpot IFN-γ ELISpot assays were performed using a commercial kit from Abcam (ab64029) following the manufacturer’s recommendations. Cells were stimulated with 1.25 μg/ml anti-CD28 (Clone

(9) Reducing cell intrinsic immunity to mRNA vaccine alters adaptive immune responses in miceMolecular therapy. Nucleic acidsDecember 12, 2023Wang Z, Jacobus EJ, Stirling DC, Krumm S, Flight KE, Cunliffe RF, Mottl J, Singh C, Mosscrop LG, Santiago LA, et al.T cell ELISpot IFN-γ ELISpot assays were performed on mouse splenocytes using a commercial kit (cat.no. ab64029, AbCam) following the manufacturer’s recommendations. Cells were stimulated with 2 μg/mL anti-CD28 (Clone

(10) An mRNA-based T-cell-inducing antigen strengthens COVID-19 vaccine against SARS-CoV-2 variantsNature communicationsMay 23, 2023Tai W, Feng S, Chai B, Lu S, Zhao G, Chen D, Yu W, Ren L, Shi H, Lu J, et al.potency. ELISpot assays of IFN-γ and IL-4 Mice ELISpot assays were performed with mouse IFN-γ (Abcam, Cat. No# ab64029) and IL-4 ELISpot kits (Mabtech, Cat. No# 3311-4HPW-10) according to the manufacturer’s instructions.

(11) Adaptation of transgene mRNA translation boosts the anticancer efficacy of oncolytic HSV1Journal for ImmunoTherapy of CancerMarch 23, 2023Huy-Dung Hoang, Aida Said, Nasana Vaidya, Victoria H Gilchrist, Kyle Malone, Usha Kabilan, Serena Topshee, Xiao Xiang, An-Dao Yang, David Olagnier, et al.penicillin/streptomycin (Fisher). IFNγ ELISPOT was done using the mouse interferon-gamma ELISPOT kit (Abcam, CAT# ab64029) according to the manufacturer’s protocol. Briefly, 100,000 splenocytes were co-cultured with/without

(12) Formulation, inflammation, and RNA sensing impact the immunogenicity of self-amplifying RNA vaccinesMolecular therapy. Nucleic acidsMarch 14, 2023Tregoning JS, Stirling DC, Wang Z, Flight KE, Brown JC, Blakney AK, McKay PF, Cunliffe RF, Murugaiah V, Fox CB, et al.exclusion. T cell ELISPOT IFN-γ ELISPOT assays were performed using a commercial kit from AbCam (ab64029) following the manufacturer’s recommendations. Cells were stimulated with 2 μg/mL anti-CD28 (Clone 37.51:

(13) Exosomes mediate Coxsackievirus B3 transmission and expand the viral tropismPLoS pathogensJanuary 1, 2023Fu Y, Xiong S.determined. ELISpot SARS-CoV-2-specific cellular immune responses in ferrets were assessed by a Murine IFN-γ ELISpot Kit (#ab64029, Abcam) following the manufacturer’s instructions. In brief, 1×105 of peripheral blood mononuclear cells

(14) Lipid nanoparticle-mediated lymph node-targeting delivery of mRNA cancer vaccine elicits robust CD8(+) T cell responseProceedings of the National Academy of Sciences of the United States of AmericaAugust 23, 2022Chen J, Ye Z, Huang C, Qiu M, Song D, Li Y, Xu Q.10% fetal bovine serum. The ELISpot assay was conducted using the Mouse Interferon gamma ELISPOT Kit (ab64029, Abcam, USA). Then 2 × 104 PBMCs cells were incubated in complete RPMI-1640 medium with or without 2

(15) FAP-Targeted Photodynamic Therapy Mediated by Ferritin Nanoparticles Elicits an Immune Response against Cancer Cells and Cancer Associated FibroblastsAdvanced functional materialsFebruary 10, 2021Zhou S, Zhen Z, Paschall AV, Xue L, Yang X, Bebin-Blackwell AG, Cao Z, Zhang W, Wang M, Teng Y, et al.producing cells in splenocytes was measured using a mouse interferon gamma ELISpot Kit (Abcam, Cat. No. ab64029) following vendor’s protocol. Splenocytes freshly isolated from animals 12 days post tumor inoculation

(16) Detecting Tumor Antigen-Specific T Cells via Interaction-Dependent Fucosyl-BiotinylationCellNovember 12, 2020Liu Z, Li JP, Chen M, Wu M, Shi Y, Li W, Teijaro JR, Wu P.ELISpot assay for TIL reactivity analysis ELISpot assays were performed using a mouse IFN-γ ELISpot kit (ab64029, Abcam). DCs were primed with indicated antigens or tumor lysates as described above. Different subsets

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Mouse Interferon gamma ELISPOT Kit from Abcam

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Mouse Interferon gamma ELISPOT Kit from Abcam

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