Fig 1: Dose-response inhibition of mitochondrial protein synthesis of clinical oxazolidinones. MPS inhibition was quantified using the MitoBiogenesis in-cell ELISA kit (ab110217; Abcam) in HepG2 cells (see Fig. S1 in the supplemental material). The assay compared the production of two proteins: COX-1, synthesized by mitochondrial ribosomes, and SDHA, synthesized by cytoplasmic ribosomes. Cell viability was measured with the Janus green (JG) stain. Raw data were interpreted per the manufacturer’s instructions and plotted using GraphPad Prism to calculate the IC50 values of the relative inhibition of COX-1/SDH-A synthesis, and the percent cell viability. Chloramphenicol (CAM) and clarithromycin (CLR) were used as the positive and negative controls, respectively. The oxazolidinone abbreviations are as provided in Table 1. 95% CI, 95% confidence interval.