Fig 1: The IP-10 release from macrophages activated with LPS and INF? is reduced by hemin. Macrophages were incubated for (A) 24 or (B) 72 h with vehicle in the presence or absence of 10 µM hemin. Macrophages were also activated for (A) 24 or (B) 72 h with vehicle or 5 pg/mL LPS and 10 ng/mL INF? in the presence or absence of 10 µM hemin. IP-10 levels in the media were quantified at 450 nm and normalized to the cell number. Values with different superscript letters (a and b) indicate significant differences (p < 0.05) between groups. For all bars with the same letter, the difference between the means is not statistically significant. IP-10, interferon-?-inducible protein 10.
Fig 2: 3,4DHPAA and 4HPAA induce IP-10 release in macrophages and hemin reduces IP-10 release in macrophages. Levels of IP-10 in the media of (A) macrophages and (B) activated macrophages incubated with 10 µM 3,4DHPAA or 10 µM 4HPAA, in the presence or absence of 10 µM hemin for 72 h. Values with different superscript letters (a and b) indicate significant differences (p < 0.05) between groups. For all bars with the same letter, the difference between the means is not statistically significant. 3,4DHPAA, 3,4-dihydroxyphenylacetic acid; 4HPAA, 4-hydroxyphenylacetic acid; IP-10, interferon-?-inducible protein 10.
Supplier Page from Abcam for Human IP-10 ELISA Kit (CXCL10)