Fig 1: WT1-AS and p53 expression levels are positively correlated in patients with osteoporosis. Plasma levels of (A) WT1-AS and (B) p53 in plasma were measured using ELISA. Data between osteoporosis and control groups were compared by performing an unpaired t-test. Correlations between WT1-AS and p53 in (C) patients with osteoporosis and (D) healthy controls were analyzed using Pearson's correlation coefficient. *P<0.05. WT1-AS, long non-coding WT1 antisense RNA; p53, cellular tumor antigen p53.
Fig 2: WT1-AS promotes osteoblast apoptosis through p53. The effects of WT1-AS and p53 overexpression as well as WT1-AS siRNA silencing on osteoblast apoptosis were analyzed by performing a cell apoptosis assay. Data among groups were compared by performing one-way ANOVA followed by Tukey's test, *P<0.05. siRNA, small interfering RNA; C, control; NC, negative control; WT1-AS, long non-coding WT1 antisense RNA; p53, cellular tumor antigen p53.
Fig 3: LINC00673 inhibited apoptosis of h1RPE7 and ARPE-19 cells under a high glucose environment via p53. h1RPE7 cells (A) and ARPE-19 cells (B) were treated with 30 and 60 mM D-glucose for 24h, followed by the analysis of cell apoptosis. Compared to C and NC groups, LINC00673 overexpression decreased while LINC00673 siRNA silencing increased the apoptotic rate of RPECs. In addition, p53 overexpression reduced the effect of LINC00673 overexpression. *p<0.05.
Fig 4: Summary of the results found in this study integrated into the experimentally based telomere-mitochondrial axis of biological aging hypothesis. DNA damage and telomere shortening activate p53 leading to growth arrest, senescence or apoptosis. p53 might also impair mitochondrial function and mitochondrial DNA content indirectly through suppression of PGC-1a – one of the master regulators of the mitochondria – leading to mitochondrial comprise and increased ROS levels, which leads to more DNA damage including telomere shortening. p53 and PGC-1a could therefore be central players in the association between telomere length and mitochondrial DNA content and subsequently in the aging process. Solid lines represent significant associations between age-related or protein markers, while non-significant associations are represented by dotted lines. p53 and PGC-1a levels were only measured in cord blood, while TL and mtDNAc were measured in both cord blood and placental tissue. Abbreviations: p53: tumor suppressor protein 53; PGC-1a: peroxisome proliferator-activated receptor gamma co-activator 1 alpha protein. Figure based on the experimental work of Sahin et al. [5, 33].
Fig 5: Altered expression levels of WT1-AS and p53 separate osteoporosis patients from healthy controls. The potential application of plasma (A) WT1-AS and (B) p53 for the diagnosis of osteoporosis was explored by plotting a receiver operating characteristic curve. WT1-AS, long non-coding WT1 antisense RNA; p53, cellular tumor antigen p53.
Supplier Page from Abcam for Human p53 ELISA Kit