Fig 1: SERPINC1 (antithrombin gene) expression and quantification of AT and TAT in ECs and fibroblasts. (a) The expression levels of SERPINC1 in each cell type were analyzed by real time-PCR and quantified relative to HUVEC levels after normalization to GAPDH in each PCR analysis. Data shown are means + SD from three separate RNA extractions for each cell type. *p < 0.0001. (b) Levels of AT via ELISA were quantified in supernatant of HUVECs (n = 3), LSECs (n = 4), GMVECs (n = 3), and fibroblasts (n = 5). (c) Quantification of TAT in the lysate/cell membrane fraction of HUVECs, LSECs, GMVECs, and fibroblasts (n = 3 for each cell type). *p < 0.05.
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