Description
The competitive Dopamine ELISA kit uses the microtiter plate format. Dopamine is bound to the solid phase of the microtiter plate. Acylated dopamine from the sample and solid phase bound dopamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free Antigens and free Antigens-antiserum complexes are removed by washing. The antibody bound to the solid phase dopamine is detected by anti-rabbit IgG / peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase dopamine is inversely proportional to the dopamine concentration of the sample