Description
Rapid EDDP Test is an immunochromatographic one-step test intended for the qualitative determination of methadone’s primary metabolite, 2-Ethylidine-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in human urine specimen. Rapid EDDP Test is based on the principle of the highly specific immunochemical reactions of antibodies and antigens, which are used for the analysis of specific compounds in human urine. The drug-protein conjugate competes for limited antibody binding sites with drugs that may be present in the urine. When drug is present in the urine specimen, it competes with drug protein conjugate for the limited amount of antibody-dye conjugate. If the amount of free drug is equal to or greater than the cut-off, it will prevent binding of the drug protein conjugate to the antibody-dye conjugate. Therefore, a positive urine specimen will not show a colored band on the test line zone, indicating a positive result, while the presence of a colored band indicates a negative result. A control line is present in the test window to serve as procedural control. This colored band should always appear on the control line zone if the test device is stored in good condition and the test is performed properly.