Description
For the quantitative determination of the total lipid index in serum using the sulfo-phospho-vanillin colorimetric method. In blood, at least 95% of the lipids exist in combination with protein. These lipoproteins can be quantitated by disrupting this complex. This test is used as a screening method for hyperlipidemia. The sulfo-phospho-vanillin (SPV) method for the colorimetric determination of the serum total lipids was described by Charbrol et al and modified by several investigators by omitting phosphoric acid, shortening reaction time, and increasing reagent stability. Our reagent is based on all of these modifications. Lipids react with sulfuric acid to form carbonium ions, which subsequently react with the vanillin phosphate ester to yield a purple complex that is measured photometrically.