Description
For the quantitative determination of lactate dehydrogenase activity in human serum. The enzyme lactate dehydrogenase (LDH-L) is distributed in tissues particularly heart, liver, muscle, and kidney. The enzyme found in circulation is a mixture of five isoenzymes based on their mobility. Elevated serum levels of LDH-L are found in serum in myocardial infarction, liver disease, renal disease, certain forms of anemia, malignant diseases, and progressive muscle dystrophy. LDH catalyzes the oxidation of lactate to Pyruvate in the presence of NAD, which is subsequently reduced to NADH. The rate of NADH formation measured at 340 nm is directly proportional to serum LDH-L activity.