Description
Rapid Opiates test card is an immunochromatography based one step in vitro test. It is designed for qualitative determination of Opiates and its metabolite in human urine specimens. The presence of morphine in human urine above a cut-off level of 2,000ng/ml can be detected. Rapid Opiates Test Card is based on the principle of specific immunochemical reaction between antibodies and antigens to analyze particular compounds in human urine specimen. The assay relies on the competition for binding antibody between morphine-protein conjugate and free morphine which may be present in the urine specimen being tested. When morphine is present in the urine specimen, it competes with morphine-protein conjugate which is immobilized on the nitrocellulose membrane for the limited amount of anti-morphine-dye conjugate. When the amount of morphine is equal or more than the cut-off, 2,000ng/ml, it will prevent the binding of morphine-protein conjugate to the antibody. Therefore, a positive urine specimen will not show a colored band on the test line zone, indicating a positive result, while the presence of a colored band indicates a negative result.