Fig 1: Correlation of overall patient survival rate with plasma ANGPTL4 concentration in TC. The overall survival rate was evaluated for groups of patients with TC classified by ANGPTL4 concentration >9.1 ng/ml (n=20; solid line) or =9.1 ng/ml (n=20; dashed line). Statistical analysis was performed using the Kaplan-Meier method and verified using the log-rank test. The table of numbers at risk at the bottom of the figure indicates the number of patients living just before the time point. ANGPTL4, angiopietin-like 4; TC, tongue cancer.
Fig 2: ANGPTL4 plasma concentrations in patients with TC with or without lung metastasis. ANGPTL4 plasma concentrations were compared between patients with TC with and without lung metastasis. Open circles indicate individual ANGPTL4 plasma concentrations of patients with TC (n=20 each). Closed circles and bars denote averages and standard deviation values, respectively. Statistical analysis was performed using an unpaired Student's t-test. *P<0.001. The dashed line indicates the optimal cut-point of the plasma ANGPTL4 concentration for prediction of TC lung metastasis. The optimal cut-point was determined by bootstrapped receiver operating characteristic analysis under Liu's method using 1,000 bootstrap samples. ANGPTL4, angiopietin-like 4; TC, tongue cancer.
Fig 3: Promotive effects of ANGPTL4 on various stages of tongue cancer cell metastasis from the tongue to lungs. Black arrows indicate the stages of metastasis promoted by ANGPTL4. ANGPTL4, angiopoietin-like 4.
Fig 4: Association of overall patient survival rate with ANGPTL4 expression in TC. The overall survival rate was evaluated for groups of patients with TC classified by high (n=15; solid line) or low (n=33; dashed line) rates of ANGPTL4 expression in their collected TC tissue samples. Statistical analysis was performed using the Kaplan-Meier method and verified using the log-rank test. The table of numbers at risk at the bottom of the figure indicates the number of patients living just before the time point. ANGPTL4, angiopietin-like 4; TC, tongue cancer.
Fig 5: Gene Expression Analysis of Inflammatory Mediators and Transcription Factors after c-Myc knockdown. A. Time-dependent changes in the expression of pro-inflammatory genes Angptl4, Cxcl12, Mdk, Tgfb2, Tnfsf15 and Vcam1 in endothelial cells three (black bars) and six (gray bars) days after c-Myc knockdown. B. Transcription factor gene expression profiling 6 days after c-Myc knockdown. Results are expressed as fold-change relative to NS-Control. Gene expression data were normalized to at least two endogenous control genes. C. Network pathway analysis of inflammatory mediators and transcription factors induced by c-Myc knockdown. (n = 4–5) (*p<0.05, **p<0.005).
Supplier Page from Abcam for Human ARP4 ELISA Kit (Angiopoietin-Like 4)