Fig 1: Expression levels of leptin and adiponectin in the WT and FKO mice.(A) qPCR analysis for Lep in the sWAT from WT and FKO mice fed on regular or HFD (n = 5 -10 per group, each point represents a biology replicate, representative of three repeats). Data are represented as mean ± SEM, one-way ANOVA, **P < 0.01, ***P < 0.001. (B) qPCR analysis for Adipoq mRNA level in the sWAT from WT and FKO mice fed on regular chow or HFD. (n = 5 in each group, each point represents a biology replicate, representative of three repeats). Data are represented as mean ± SEM, one-way ANOVA.
Fig 2: Effect of UAL on the expression of functional genes in 3T3-L1 cells. 3T3-L1 cells were differentiated for 4 days and treated with UAL for 24 h. Metformin was used as positive control. Relative expression of Pparg (A), Cebpa (B), Adipoq (C), Ppara (D), Srebf1 (E), Acaca (F), Fasn (G) Lep (H), Ppargc1a (I), Ucp1 (J) mRNA transcripts is shown. Values are expressed as mean ± SEM of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with DIFFERENTIATED (ANOVA with Dunnett´s post hoc test).
Supplier Page from Abcam for Mouse Leptin ELISA Kit