Fig 1: Expression levels of leptin and adiponectin in the WT and FKO mice.(A) qPCR analysis for Lep in the sWAT from WT and FKO mice fed on regular or HFD (n = 5 -10 per group, each point represents a biology replicate, representative of three repeats). Data are represented as mean ± SEM, one-way ANOVA, **P < 0.01, ***P < 0.001. (B) qPCR analysis for Adipoq mRNA level in the sWAT from WT and FKO mice fed on regular chow or HFD. (n = 5 in each group, each point represents a biology replicate, representative of three repeats). Data are represented as mean ± SEM, one-way ANOVA.
Fig 2: Effect of UAL on the expression of functional genes in 3T3-L1 cells. 3T3-L1 cells were differentiated for 4 days and treated with UAL for 24 h. Metformin was used as positive control. Relative expression of Pparg (A), Cebpa (B), Adipoq (C), Ppara (D), Srebf1 (E), Acaca (F), Fasn (G) Lep (H), Ppargc1a (I), Ucp1 (J) mRNA transcripts is shown. Values are expressed as mean ± SEM of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with DIFFERENTIATED (ANOVA with Dunnett´s post hoc test).
Fig 3: Effect of P. gingivalis bacteria on plasma and adipose tissue inflammatory markers in obese db/db mice. Obese db/db mice were intravenously injected with vehicle (Control) or P. gingivalis bacteria (P. g bacteria) at 107 CFU. After 4 h, CRP levels in plasma (a) and liver (b) were measured by ELISA kit. Levels of IL-6, MCP-1 and TNFα were determined in subcutaneous (SAT, (c–e)) and visceral (VAT, (f–h)) adipose tissues by ELISA kits. Data are expressed as mean ± SEM (n = 5). * p < 0.05, ** p < 0.01, *** p < 0.005 as compared to Control.
Fig 4: Effect of P. gingivalis bacteria on antioxidant enzyme activities in the adipose tissues of obese db/db mice. Obese db/db mice were intravenously injected with vehicle (Control) or P. gingivalis bacteria (P. g bacteria) at 107 CFU. After 4 h, the total SOD and catalase activities were measured in both subcutaneous (SAT, (a,b)) and visceral (VAT, (c,d)) adipose tissues by fluorometric assays. Data are expressed as mean ± SEM (n = 5). ** p < 0.01 as compared to Control.
Fig 5: Effect of P. gingivalis bacteria on triglyceride and cholesterol levels in plasma, liver and adipose tissues in obese db/db mice. Obese db/db mice were intravenously injected with vehicle (Control) or P. gingivalis bacteria (P. g bacteria) at 107 CFU. After 4 h, triglyceride and total cholesterol levels were measured in plasma (a,b) and liver (c,d) using colorimetric quantification kits. Triglyceride levels in subcutaneous (SAT, (e)) and visceral (VAT, (f)) adipose tissues were measured using colorimetric quantification kits. Data are expressed as mean ± SEM (n = 5). * p < 0.05, *** p < 0.005 as compared to Control.
Supplier Page from Abcam for Mouse Leptin ELISA Kit