Fig 1: miR-7-5p downregulates NF-?B transcriptional activity and downstream NF-?B targets in melanoma cellsA. Luciferase reporter assay with WM266-4, A2058 and 1205Lu cells that were transfected with NF-?B cignal luciferase reporter or negative control luciferase reporter, and either miR-7-5p or miR-NC precursor molecules (10 nM), RelA siRNA (si-RelA7, positive control for assay) or negative control siRNA (si-NC) at 5 nM. *, p-value < 0.01. B. ELISA analysis of secreted and intracellular expression of IL-1ß, IL-6 and IL-8 in 1205Lu cells 3 d post-transfection with either miR-7-5p or miR-NC precursor molecules, or si-RelA7 or si-NC. *, p-value < 0.01.
Fig 2: CyaA affects intracellular cAMP concentration and IL-6 secretion in human airway epithelial cells. (a) HNEC, HTEC and HBEC3-KT were incubated with CyaA or CyaA-AC– and intracellular cAMP concentration was analysed (n = 4). (b) Quantification of human IL-6 secreted by HNEC, HTEC and HBEC3-KT populations after treatment with CyaA or CyaA-AC– was assayed by ELISA of the supernatants (n = 3). (c) Quantification of human IL-6 secreted by human nasal mucosa tissue models after treatment with CyaA or CyaA-AC– (n=3). All data are presented as means (bars) + SEM (error bars). Differences after CyaA incubation compared to the control group (TUC) are indicated (*P = 0.05, $P > 0.05 = 0.1, ANOVA). Statistically significant differences of IL-6 basal levels are indicated (#P < 0.05, ANOVA). Significant cell type-specific differences are indicated (&P < 0.05, ANOVA).
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