Fig 1: ELISA analysis of inflammatory markers including interleukins (IL1-β, IL-6, IL-6R, IL-8, IL-10, and IL-13), cellular adhesion and inflammation-associated marker [intercellular adhesion molecule (ICAM-1)] and chemokines (CCL-2, CCL-4, CCL-5, and CXCL-10) and TIMP-2 in cells extraction of HUVEC (A) and THP-1 (B) treated with PBS, TNF-α (10 ng/ml), uEV (10 µg/ml total proteins), and tEV (10 µg/ml total proteins). Values are given as mean ± SD of three independent biological individuals in two technical replicates (n = 6). p-Values were calculated by one-way analysis of variance with a multiple comparisons test (Tukey’s multiple comparison test) and *p < 0.05 (PBS vs treatments) and #p < 0.05 (uEV vs tEV) were considered as statistically significant.
Fig 2: The immunomodulatory content of extracellular vesicles (EV) derived from TNF-α stimulated HUVEC (tEV) and non-stressed (unstimulated) cells (uEV). (A) A representative image of membrane based inflammation arrays C1 and C2 of uEV and tEV. (B) Relative densitometry of each protein was obtained using Image J software. *p Values <0.05 was considered as statistically significant. (C) ELISA analysis of GM-CSF, IL1-β, IL-4, IL-6, IL-6R, IL-8, IL-10, IL-13, intercellular adhesion molecule (ICAM)-1, CCL-2, CCL-4, CCL-5, CXCL-10, and TIMP-2 were done on 1 µg total protein of endothelial cells (EC)-derived uEV, tEV, and cEV. For data of ELISA, *p values <0.05 was considered as statistically significant. Values are given as mean ± SD of three independent biological individuals in two technical replicates (n = 6).
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