BrdU Cell Proliferation Assay Kit 6813S from Cell Signaling Technology

Product Specs
ItemBrdU Cell Proliferation Assay Kit
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number6813S
Quantity1 Kit
Research AreaCell Cycle / Checkpoint Control
Detection TargetBrdU
ApplicationsFUNC

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Cell-based screen identifies porphyrins as FGFR3 activity inhibitors with therapeutic potential for achondroplasia and cancerJCI InsightNovember 22, 2023Yun-Wen Lin, Hsiao-Jung Kao, Wei-Ting Chen, Cheng-Fu Kao, Jer-Yuarn Wu, Yuan-Tsong Chen, Yi-Ching Leeproliferation was determined using a BrdU cell proliferation assay kit (Cell Signaling Technology, 6813) according to the manufacturer’s protocol. Cells were seeded at a density of 5 × 104 cells/well in 96-well

(2) RAR-Dependent and RAR-Independent RXR Signaling in Stem-like Glioma CellsInternational Journal of Molecular SciencesNovember 17, 2023Amanda Dabrock, Natalie Ernesti, Florian Will, Manaf Rana, Nadja Leinung, Phillip Ehrich, Volker Tronnier, Christina Zechelcontinued using the BrdU Cell Proliferation Assay Kit marketed by Cell Signaling (Danvers, MA, USA, #6813S). Due to the suspension of deliveries, another BrdU kit (BrdU Cell Proliferation Assay Kit, #6813, Cell Signaling

(3) New Insights on the Progesterone (P4) and PGRMC1/NENF Complex Interactions in Colorectal Cancer ProgressionCancers (Basel)October 20, 2023Joanna Kamińska, Olga Martyna Koper-Lenkiewicz, Donata Ponikwicka-Tyszko, Weronika Lebiedzińska, Ewelina Palak, Maria Sztachelska, Piotr Bernaczyk, Justyna Dorf, Katarzyna Guzińska-Ustymowicz, Konrad Zaręba, et al.was the BrdU Cell Proliferation Assay Kit (Cell Signaling Technology, Danvers, MA, USA, catalog #: 6813). The medium containing the drugs was changed every 24 h, while the control groups were treated with

(4) Triple negative breast cancer metastasis is hindered by a peptide antagonist of F11R/JAM‑A proteinCancer Cell IntAugust 11, 2023Radosław Bednarek, Dagmara W. Wojkowska, Marcin Braun, Cezary Watala, Moro O. Salifu, Maria Swiatkowska, Anna BabinskaELISA (enzyme-linked immunosorbent assay) with BrdU Cell Proliferation Assay Kit (Cell Signaling, #6813) according to the manufacturer’s protocol. The absorbance was read at 450 nm (A450) by the Wallac 1420

(5) Clinically Defined Mutations in MEN1 Alter Its Tumor-suppressive Function Through Increased Menin TurnoverCancer Research CommunicationsJuly 24, 2023Suzann Duan, Sulaiman Sheriff, Uloma B. Elvis-Offiah, Brandon L. Witten, Travis W. Sawyer, Sinju Sundaresan, Tomasz Cierpicki, Jolanta Grembecka, Juanita L. Merchantbromodeoxyuridine (BrdU) incorporation as described by the manufacturer (Cell Signaling Technology, #6813). Cells were seeded at a density of 10,000 cells in 96-well plates and transfected after 24 hours with

(6) Quantitative Proteomics Reveals Transforming Growth Factor β Receptor Targeted by Resveratrol and Hesperetin Coformulation in Endothelial CellsACS OmegaMay 9, 2023Aktham Mestareehi, Hainan Li, Xiangmin Zhang, Sai Pranathi Meda Venkata, Ruchi Jaiswal, Fu-Shin Yu, Zhengping Yi, Jie-Mei Wangpreviously.9 Cell proliferation was evaluated using the BrdU Cell Proliferation Assay Kit (Cell Signaling, #6813). Cell apoptosis was determined by Annexin V-PI staining using a Countess II FL Automated Cell Counter

(7) Pleiotropic effects of BAFF on the senescence-associated secretome and growth arresteLifeApril 21, 2023Martina Rossi, Carlos Anerillas, Maria Laura Idda, Rachel Munk, Chang Hoon Shin, Stefano Donega, Dimitrios Tsitsipatis, Allison B Herman, Jennifer L Martindale, Xiaoling Yang, et al.manufacturers’ instructions (XTT Assay Kit, Abcam, ab232856; BrdU Cell Proliferation Assay Kit, CST, #6813). Transfection and nucleofection Silencing interventions in all the cells analyzed were performed

(8) RIDR-PI-103, ROS-activated prodrug PI3K inhibitor inhibits cell growth and impairs the PI3K/Akt pathway in BRAF and MEK inhibitor-resistant BRAF-mutant melanoma cellsAnti-Cancer DrugsApril 1, 2023Hima Patel, Rosalin Mishra, Adam Wier, Nazanin Mokhtarpour, Edward J. Merino, Joan T. GarrettTDR cells was analyzed using BrdU cell proliferation assay kit from Cell Signaling Technology (cat# 6813; Danvers, Massachusetts, USA). Briefly, equal number of TDR cells (2 × 104 cells/well) were plated and

(9) Biomanufacturing Recombinantly Expressed Cripto-1 Protein in Anchorage-Dependent Mammalian Cells Growing in Suspension Bioreactors within a Three-Dimensional Hydrogel MicrocarrierGelsMarch 18, 2023Rachel Lev, Orit Bar-Am, Yoni Lati, Ombretta Guardiola, Gabriella Minchiotti, Dror Seliktarng/mL. Cell proliferation was quantified using a BrdU Cell Proliferation Assay kit (Cell Signaling, 6813) according to the manufacturer’s instructions. 4.9. Ki67 Immunostaining Proliferation Assay A Ki67

(10) ANGPTL4 stabilizes atherosclerotic plaques and modulates the phenotypic transition of vascular smooth muscle cells through KLF4 downregulationExperimental & molecular medicineFebruary 1, 2023Cho DI, Ahn MJ, Cho HH, Cho M, Jun JH, Kang BG, Lim SY, Yoo SJ, Kim MR, Kim HS, et al.Additionally, HAoSMC proliferation was analyzed using a colorimetric bromodeoxyuridine (BrdU) ELISA kit (6813, Cell Signaling Technology) according to the manufacturer’s manual. Cells were seeded into 96-well plates

(11) Inhibition of GPR39 restores defects in endothelial cell-mediated neovascularization under the duress of chronic hyperglycemia: Evidence for regulatory roles of the sonic hedgehog signaling axisProceedings of the National Academy of Sciences of the United States of AmericaJanuary 3, 2023Meda Venkata SP, Li H, Xu L, Koh JY, Nguyen H, Minjares M, Li C, Kowluru A, Milligan G, Wang JM.Assays). EC proliferation was evaluated using the BrdU Cell Proliferation Assay Kit (Cell Signaling, #6813). Optical density at 450 nm was recorded using a BioTek Epoch spectrophotometer. Cell migration was

(12) MiR-106b-5p regulates esophageal squamous cell carcinoma progression by binding to HPGDBMC CancerDecember 1, 2022Fan Yang, Zhanwen Sun, Dengyun Wang, Tian DuAfter another 8 h, serum was added back to the cells. The BrdU Cell Proliferation Assay Kit (Cat#: 6813, CST, Danvers, MA, USA) was used to label cells for 8–12 h without removing the treatment media. Finally,

(13) miR-4324 inhibits ovarian cancer progression by targeting FEN1Journal of Ovarian ResearchDecember 1, 2022Haixia Wu, Youliang Yan, Jialin Yuan, Mengze Luo, Yingjian Wang450 nm was then determined using a microplate reader (Thermo, USA). BrdU assay The BrdU kit (Cat#: 6813, CST, USA) was used to identify cell proliferation. CaOV3 and OVCAR3 cells (5 × 103) were cultured in

(14) Combinatorial prophylactic effect of phlorotannins with photobiomodulation against tracheal stenosisiScienceNovember 18, 2022Yeachan Lee, Seong-Yeong Heo, Hyoung Shin Lee, Sun-ju Oh, Hyeonsoo Kim, Seonghee Lim, Hwarang Shin, Won-Kyo Jung, Hyun Wook KangThe cell proliferation was analyzed using a bromodeoxyuridine (BrdU) Cell Proliferation Assay Kit (#6813S; Cell Signaling Technology, MA, USA) according to the manufacturer’s instructions. Each experiment was

(15) Co-targeting CDK2 and CDK4/6 overcomes resistance to aromatase and CDK4/6 inhibitors in ER+ breast cancernpj Precision OncologySeptember 24, 2022Abeer J. Al-Qasem et al.senescence-β Galactosidase assay Cell proliferation was assessed using a BrdU cell proliferation kit (6813, Cell Signaling Technology), apoptosis was assessed using the Cell Death Detection ELISAPlus kit (11774425001,

(16) CircGSAP regulates the cell cycle of pulmonary microvascular endothelial cells via the miR-942-5p sponge in pulmonary hypertensionFrontiers in Cell and Developmental BiologyAugust 11, 2022Yuanyuan Sun et al.assay. Cells (2 × 103) were seeded onto 96-well dishes in ECM and maintained at 37°C. A BrdU Assay (6813, Cell Signaling Technology, United States) was performed the assay following the manufacturer’s instructions.

(17) Functional Implications of Estrogen and Progesterone Receptors Expression in Adenomyosis, Potential Targets for Endocrinological TherapyJournal of Clinical MedicineJuly 28, 2022Maria Sztachelska, Donata Ponikwicka-Tyszko, Lydia Martínez-Rodrigo, Piotr Bernaczyk, Ewelina Palak, Weronika Półchłopek, Tomasz Bielawski, Sławomir Wołczyńskiproliferation assay was evaluated in adenomyosis primary cell lines using BrdU Cell Proliferation Assay kit (#6813, Cell Signaling, Danvers, MA, USA) and performed following the manufacturer’s instructions. Cells were

(18) A Label-Free Cell Sorting Approach to Highlight the Impact of Intratumoral Cellular Heterogeneity and Cancer Stem Cells on Response to TherapiesCellsJuly 22, 2022Céline Hervieu, Mireille Verdier, Elodie Barthout, Gaëlle Bégaud, Niki Christou, Magali Sage, Julie Pannequin, Serge Battu, Muriel Mathonnetprimary cultures used. Cell proliferation was assessed using the BrdU cell proliferation assay kit (no. 6813, Cell signaling technology, France) according to the manufacturer’s instructions. Absorbance was then

(19) MPA alters metabolic phenotype of endometrial cancer-associated fibroblasts from obese women via IRS2 signalingPLOS ONEJuly 11, 2022Intan Sofia Omar, Amira Hajirah Abd Jamil, Noor Azmi Mat Adenan, Ivy Chungcontrol. 2.6. 5-bromo- 2-deoxyuridine (BrdU) cell proliferation assay The BrdU cell proliferation assay (Cat#6813, Cell Signaling Technology, MA, USA) was used to measure cell proliferation rate in CN and CO for 24

(20) Galectin-1 activates carbonic anhydrase IX and modulates glioma metabolismCell Death & DiseaseJune 30, 2022Maheedhara R. Guda, Andrew J. Tsung, Swapna Asuthkar, Kiran K. VelpulaFluoview, Minneapolis, MN). BrdU (Bromodeoxyuridine) cell proliferation assay Using a BrdU Kit (#6813, Cell Signaling Technology Danvers, MA, USA), we examined the effect of silencing shGal-1 on GSC proliferation.

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BrdU Cell Proliferation Assay Kit

BrdU Cell Proliferation Assay Kit from Cell Signaling Technology

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Cell Signaling Technology

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BrdU Cell Proliferation Assay Kit from Cell Signaling Technology

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