Human/Mouse Total HIF-1 alpha/HIF1A DuoSet IC ELISA DYC1935-2 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman/Mouse Total HIF-1 alpha/HIF1A DuoSet IC ELISA
CompanyR&D Systems, a Bio-Techne Brand
Price $495.00Supplier Page View Company Product Page
Catalog NumberDYC1935-2
Quantity1 Kit (for 2 Plates) (also 1 Kit (for 5 Plates), 1 Pack (for 15 Plates))
ApplicationsELISA
Detection Range125.00 - 8000 pg/mL
ReactivityHuman, Mouse
Detection TargetHIF-1 alpha/HIF1A
Assay Time4 hours 40 mins (after plate preparation)
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Namehypoxia inducible factor 1 subunit alpha
NCBI Gene AliasesHIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) A transparent osmotic wound dressing ATKPAD promotes the healing process and regulates hypoxia-dependent inflammatory responsesScientific ReportsMarch 6, 2026Yuki Sato, Eiki Ito, Hiromasa Tanno, Takashi Kanno, Ryuto Arai, Koji Sato, Ayato Kaneta, Wakana Kamada, Ikue Sone, Ko Sato, et al.using a Human/Mouse Total HIF-1 alpha/HIF1A DuoSet IC ELISA (R&D Systems Minneapolis, MN, USA; Cat# DYC1935-2) according to the manufacturer’s instructions. The analysis was conducted at 24 h after wounding. Statistical

(2) A high fraction of inspired oxygen does not mitigate atelectasis-induced lung tissue hypoxia or injury in experimental acute respiratory distress syndromeScientific reportsJanuary 14, 2025Tojo K, Yazawa T.buffer containing a protease inhibitor cocktail. The concentration of HIF-1α was quantified by ELISA (DYC1935-2, R&D Systems), and the value was normalized to the total protein concentration determined by the BCA

(3) Synergistic Effects of Mistletoe Lectin and Cisplatin on Triple-Negative Breast Cancer Cells: Insights from 2D and 3D In Vitro ModelsInternational journal of molecular sciencesJanuary 3, 2025Lyu SY, Meshesha SM, Hong CE.measured using the Human VEGF Quantikine ELISA Kit (Cat# DVE00) and Human HIF-1α DuoSet ELISA (Cat# DYC1935-2, R&D Systems, Minneapolis, MN, USA), respectively. All assays were performed according to the manufacturer’s

(4) The effect of sulthiame on potential biomarkers in moderate to severe obstructive sleep apnoeaERJ Open ResearchDecember 2, 2024Erik Hoff, Saliha Musovic, Ali M. Komai, Ding Zou, Christian Strassberger, Kaj Stenlöf, Ludger Grote, Jan Hednerused for sandwich ELISA to determine HIF-1α concentration (ng·mL−1) with a commercially available kit (DYC1935-2; Bio-Techne, Minneapolis, MN, USA). The assay was performed according to the ELISA kit protocol. Statistical

(5) Mitochondrial antioxidants abate SARS-COV-2 pathology in miceProceedings of the National Academy of Sciences of the United States of AmericaJuly 23, 2024Guarnieri JW, Lie T, Albrecht YES, Hewin P, Jurado KA, Widjaja GA, Zhu Y, McManus MJ, Kilbaugh TJ, Keith K, et al.Human/Mouse Total HIF-1 alpha/HIF-1 alpha DuoSet IC enzyme-linked immunosorbent assay (ELISA) (R&D Systems, DYC1935-2); IFNβ using the Mouse IFN beta ELISA Kit (Abcam, ab252363); and IL1β utilizing the Mouse IL-1 beta ELISA Kit

(6) Harnessing EGLN1 Gene Editing to Amplify HIF-1α and Enhance Human Angiogenic ResponsebioRxiv : the preprint server for biologyMay 30, 2023Shams S, Stilhano RS, Silva EA.

(7) Effects of Short-Term Phosphate Loading on Aerobic Capacity under Acute Hypoxia in Cyclists: A Randomized, Placebo-Controlled, Crossover StudyNutrientsJanuary 6, 2022Płoszczyca K, Chalimoniuk M, Przybylska I, Czuba M.

(8) Nociceptive sensory neurons promote CD8 T cell responses to HSV-1 infectionNature communicationsMay 18, 2021Filtjens J, Roger A, Quatrini L, Wieduwild E, Gouilly J, Hoeffel G, Rossignol R, Daher C, Debroas G, Henri S, et al.

(9) Acid Sensing Ion Channel 2a Is Reduced in the Reduced Uterine Perfusion Pressure Mouse Model and Increases Seizure Susceptibility in Pregnant MiceCellsMay 8, 2021Jones-Muhammad M, Shao Q, Cain-Shields L, Shaffery JP, Warrington JP.Samples Placental homogenates were assayed in duplicate on a HIF1α ELISA kit (Human/Mouse total HIF1A ELISA, Cat #: DYC1935-2, R&D Systems) following the manufacturer’s directions. Samples were run undiluted. Measured concentrations

(10) Treatment of hypoxia-dependent cardiovascular diseases by myo-inositol trispyrophosphate (ITPP)-enhancement of oxygen delivery by red blood cellsJournal of cellular and molecular medicineFebruary 1, 2020Oknińska M, El-Hafny-Rahbi B, Paterek A, Mackiewicz U, Crola-Da Silva C, Brodaczewska K, Mączewski M, Kieda C.

(11) Qingre Baidu mixture-induced effect of AI-2 on Staphylococcus aureus and Pseudomonas aeruginosa biofilms in chronic and refractory woundsExperimental and therapeutic medicineMay 1, 2019Shan W, Wang Y, Zhang Z, Xing J, Xu J, Xiao W, Shen Y, Guo S, Que H.Corporaion, Tokyo, Japan). ELISA ELISA kits were used to determine the concentration of HIF-1α (cat. no. DYC1935-2; R&D Systems, Inc.), HIF-2α (cat. no. DYC1997-2; R&D Systems, Inc.), HIF-3α (Spbio, Wuhan, China) and

(12) Consequences of Hypoxia for the Pulmonary Alveolar Epithelial Cell Innate Immune ResponseJournal of immunology (Baltimore, Md. : 1950)December 1, 2018Sturrock A, Woller D, Freeman A, Sanders K, Paine R 3rd.

(13) HIF prolyl hydroxylase inhibition protects skeletal muscle from eccentric contraction-induced injurySkeletal muscleNovember 13, 2018Billin AN, Honeycutt SE, McDougal AV, Kerr JP, Chen Z, Freudenberg JM, Rajpal DK, Luo G, Kramer HF, Geske RS, et al.

(14) Effects of Triple-Mutated Hypoxia-Inducible Factor-1α on Angiogenesis and Cardiac Function Improvement in Rats with Myocardial InfarctionCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacologyJanuary 1, 2018Li M, Cui Y, He W, Deng X, Wang Y, Cai M, Wang Y, Pei J, Mei X, Wu P.

(15) Apolipoprotein M Inhibits Angiogenic and Inflammatory Response by Sphingosine 1-Phosphate on Retinal Pigment Epithelium CellsInternational journal of molecular sciencesDecember 31, 2017Terao R, Honjo M, Aihara M.

(16) Augmentation of hypoxia-inducible factor-1-alpha in reinfused blood cells enhances diabetic ischemic wound closure in miceOncotargetDecember 26, 2017Wang H, Feng Y, Jin X, Xia R, Cheng Y, Liu X, Zhu N, Zhou X, Yin L, Guo J.isogeneic blood cells Total protein was isolated and HIF-1a levels were determined by an ELISA kit (DYC1935-2; R&D System, Los Angeles, CA, USA). Mouse hematopoietic cells were isolated from isogeneic mice, as

(17) HIF-1α and VEGF levels for monitoring hepatocellular carcinoma treatment response to transcatheter arterial chemoembolizationTranslational Cancer ResearchDecember 1, 2017Kang Liu, Lin Yang, Xiao-Ming Zhang, Yi Zhou, Tao Zhu, Nan-Dong Miao, Yong-Jun Ren, Hao Xu, Xu-Li Min, Juan Peng, et al.levels were determined using enzyme-linked immunosorbent assays (ELISAs) [human total HIF-lα Elisa kit (DYC1935-2), human VEGF Elisa kit (DVE00); R & D Systems, Inc., Minneapolis, MN 55413, USA]. Clinical data This

(18) Role of HIF-1a in regulating autophagic cell survival during cerebral ischemia reperfusion in ratsOncotargetNovember 17, 2017Guo Y.

(19) Iron-Chelating Drugs Enhance Cone Photoreceptor Survival in a Mouse Model of Retinitis PigmentosaInvestigative ophthalmology & visual scienceOctober 1, 2017Wang K, Peng B, Xiao J, Weinreb O, Youdim MBH, Lin B.

(20) Effects of Histone Deacetylase Inhibitor (Valproic Acid) on the Expression of Hypoxia-inducible Factor-1 Alpha in Human Retinal Müller CellsKorean journal of ophthalmology : KJOFebruary 1, 2017Kim YJ, Park SJ, Kim NR, Chin HS.

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Total HIF-1 alpha/HIF1A ELISA