Mouse Osteoprotegerin/TNFRSF11B DuoSet ELISA DY459 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse Osteoprotegerin/TNFRSF11B DuoSet ELISA
CompanyR&D Systems, a Bio-Techne Brand
Price $890.00Supplier Page View Company Product Page
Catalog NumberDY459
Quantity1 Kit (for 15 Plates)
ApplicationsELISA
Detection Range62.50 - 4000 pg/mL
ReactivityMouse
Detection TargetOsteoprotegerin/TNFRSF11B
Assay Time4 hours 40 minutes (after plate preparation)
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Nametumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)
NCBI Gene AliasesOCIF, Opg, TR1

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Spatially resolved osteoblast-traced transcriptomics uncovers TGF-β as a combination target with sclerostin in osteoporosisBone ResApril 2, 2026Ahyoun Choi, Ji Yeon Lee, Hyejin Yoon, Xiangguo Che, Minkyeong Choi, Yongkuk Park, Kyoungseob Shin, Hyunho Lee, Jimin Park, Sung Hye Kong, et al.and RANKL in conditioned media were measured via ELISA performed using an ELISA kit for mouse OPG (DY459, R&D systems, USA) and mouse RANKL (DY462, R&D systems, USA) according to the manufacturer’s instructions.

(2) OPG-Producing B Cells and RANKL-Expressing T Cells Define Immune Signatures Predictive of Bone Metastases in Breast CancerCancer Research CommunicationsJanuary 1, 2026Ana Carolina Monteiro, Diego D. Garcia, Ana Paula A. Fontão, Bárbara Du Rocher, Maria E. Globa Masset, Isabella N. Alves, Lucas Gonçalves Carvalho, Cecília Vianna de de Andrade, Gabriel Fidalgo, Marcos Vinicius Colaço, et al.(sAg; 50 μg/mL). Supernatants were collected after 12 hours for OPG quantification (R&D Systems, cat. #DY459, RRID: AB_2802353) and after 72 hours for RANKL quantification (R&D Systems, cat. #DY462, RRID: AB_2802355),

(3) Hydroxyapatite microspheres induce durable pleurodesis and are rapidly cleared by pleural osteoclastsJCI insightOctober 8, 2025Tanaka Y, Takahashi Y, Shindo Y, Pitstick LB, Teitelbaum SL, Zou W, Wang X, Woods JC, Wikenheiser-Brokamp KA, McCormack FX.

(4) Murine Osteoblast and Osteoclast Co-culture on Demineralized Bone Paper for Bone RemodelingBIO-PROTOCOLJune 5, 2025Seema Amin, Hyejin Yoon, Dong-Hee Choi, Yi-Hao Hsu, Jungwoo Leedetection kit (Sigma-Aldrich, catalog number: 387A) 23. Mouse OPG ELISA (R&D Systems, catalog number: DY459) 24. Mouse RANKL ELISA (R&D Systems, catalog number: DY462) 25. Ethanol (Decon Laboratories Inc., catalog

(5) AI-Assisted Label-Free Monitoring Bone Mineral Metabolism on Demineralized Bone PaperACS Biomaterials Science & EngineeringApril 14, 2025Patrick Ryan, Hyejin Yoon, Seema Amin, James J. Chambers, Jungwoo Leesecretion was performed using an enzyme-linked immunosorbent assay obtained from R&D Systems (Product Nos. DY459 and DY462). Antibodies were validated according to manufacturer’s description. Conditioned media were

(6) Downregulation of osteoprotegerin in colorectal cancer cells promotes liver metastasis via activating tumor-associated macrophageSci RepDecember 14, 2023Wataru Hirata, Yoshiro Itatani, Hideyuki Masui, Kenji Kawada, Rei Mizuno, Takamasa Yamamoto, Takuya Okamoto, Ryotaro Ogawa, Susumu Inamoto, Hisatsugu Maekawa, et al.(ELISA) OPG protein levels were measured with ELISA kits (Human OPG DuoSet DY805 and Mouse Opg Duoset DY459, R&D Systems), according to manufacturer’s protocol. CRC cell lysates were extracted in NP-40 lysis

(7) Functional and analytical recapitulation of osteoclast biology on demineralized bone paperNat CommunDecember 7, 2023Yongkuk Park, Tadatoshi Sato, Jungwoo Leeanalyzed OPG and RANKL secretion using an enzyme-linked immunosorbent assay (ELISA) kits for mouse OPG (DY459, R&D systems) and mouse RANKL (DY462, R&D systems). Antibodies were validated according to manufacturer’s

(8) Neutrophils modulate natural killer-mediated osteoclastogenesis during Aggregatibacteractinomycetemcomitans (JP2 clone) infectioniScienceAugust 18, 2023Halloun B, Hashai K, Pinto N, Musai N, Klein Y, Polak D.assays ,, "RANKL, mCSF, OPG and TNFα ELISA kits","R&D Systems, Minneapolis, Minnesota, US","DY626, DY416, DY459, DY410" TRAP kit,"Sigma-Aldrich, Rehovot, Israel",387A ,, Experimental models: Cell lines,Experimental models

(9) Long Bones Exhibit Adaptive Responses to Chronic Low‐Dose‐Rate Ionizing Radiation despite its Lifespan‐Shortening and Carcinogenic Effects on C57BL /6 MiceJBMR PlusDecember 3, 2022Masaoki Kohzaki, Akira Ootsuyama, Toshiaki Abe, Manabu Tsukamoto, Toshiyuki Umata, Ryuji Okazakiof the bone formation markers PINP and OPG were measured by ELISA (AC‐33F1, IDS) and DuoSet ELISA (DY459, R&D systems), respectively. The serum levels of the CCL2 cytokine and CXCL1 chemokine were measured

(10) Osteoprotegerin is More than a Possible Serum Marker in Liver Fibrosis: A Study into its Function in Human and Murine LiverPharmaceuticsMay 21, 2020Adhyatmika A, Beljaars L, Putri KSS, Habibie H, Boorsma CE, Reker-Smit C, Luangmonkong T, Guney B, Haak A, Mangnus KA, et al.serum, and culture supernatants were measured using a murine or human OPG DuoSet®ELISA kit (Cat. No. DY459 and DY805 for mouse and human, respectively, R&D Systems) according to the instructions provided by

(11) Fabrication of Bioactive Inverted Colloidal Crystal Scaffolds Using Expanded Polystyrene BeadsTissue Engineering Part C: MethodsMarch 1, 2020Ryan Carpenter, Dalton Macres, Jun-Goo Kwak, Katherine Daniel, Jungwoo Lee-80C. ELISAs were performed on thawed conditioned media samples using ELISA kits acquired from (DY206, DY459, DY462; R&D Systems). Methylcellulose colony-forming assay EPS scaffolds were seeded with 500,000 adherent

(12) (-)-Epigallocatechin-3-Gallate Decreases Osteoclastogenesis via Modulation of RANKL and OsteoprotegrinMolecules (Basel, Switzerland)January 3, 2019Chen ST, Kang L, Wang CZ, Huang PJ, Huang HT, Lin SY, Chou SH, Lu CC, Shen PC, Lin YS, et al.

(13) MicroRNA-374b inhibits liver cancer progression via down regulating programmed cell death-1 expression on cytokine-induced killer cellsOncology lettersApril 1, 2018Huang F, Wang B, Zeng J, Sang S, Lei J, Lu Y.the secretion of IFN-γ was measure dusing an enzyme-linked immunosorbent assay (ELISA) kit (cat no., DY459; R&D Systems, Inc.) after incubation for 72 h at 37°C according to the manufacturer's instructions.

(14) Dyslipidemia and Diabetes Increase the OPG/TRAIL Ratio in the Cardiovascular SystemMediators of InflammationDecember 14, 2016Barbara Toffoli, Bruno Fabris, Giacomo Bartelloni, Fleur Bossi, Stella BernardiJapan). Circulating levels of OPG and TRAIL were measured in the sera by ELISA (R&D Systems DuoSet #DY459 and #DY1121). 2.3. Aortic and Cardiac Structural Changes. In half of the animals in each group, aortae

(15) Comparison of maternal isocaloric high carbohydrate and high fat diets on osteogenic and adipogenic genes expression in adolescent mice offspringNutrition & MetabolismDecember 1, 2016Seyedeh Neda Mousavi, Fariba Koohdani, Farzad Shidfar, Mohamadreza Baghaban Eslaminejadthe heart. All the biochemical parameters were measured by autoanalyzer. Serum OPG (R&D system; Cat ≠ DY459), RANK-L (R&D system; Cat ≠ MTR00), Lep (R&D system; Cat ≠ MOB00) and AdipoQ (EMD Millipore; Cat ≠ EZMADP-60

(16) Marked elevation in plasma osteoprotegerin constitutes an early and consistent feature of cerebral malariaThrombosis and haemostasisApril 1, 2016O'Regan N, Moxon C, Gegenbauer K, O'Sullivan JM, Chion A, Smith OP, Preston RJ, Brophy TM, Craig AG, O'Donnell JS.

(17) Bindarit, an inhibitor of monocyte chemotactic protein synthesis, protects against bone loss induced by chikungunya virus infectionJournal of virologyJanuary 1, 2015Chen W, Foo SS, Taylor A, Lulla A, Merits A, Hueston L, Forwood MR, Walsh NC, Sims NA, Herrero LJ, et al.

(18) TLR2 deletion promotes arthritis through reduction of IL-10Journal of leukocyte biologyMay 1, 2013Huang QQ, Koessler RE, Birkett R, Perlman H, Xing L, Pope RM.

(19) Mechanisms inducing low bone density in Duchenne muscular dystrophy in mice and humansJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral ResearchAugust 1, 2011Rufo A, Del Fattore A, Capulli M, Carvello F, De Pasquale L, Ferrari S, Pierroz D, Morandi L, De Simone M, Rucci N, et al.Italy), whereas IL-6, sRANKL, and OPG were measured by ELISA kits from R&D Systems (M6000B, MTR00, and DY459). Human primary osteoblast cultures Bone fragments were obtained from healthy subjects who underwent

(20) Porphyromonas gingivalis regulates the RANKL-OPG system in bone marrow stromal cellsMicrobes and infectionNovember 1, 2008Reddi D, Bostanci N, Hashim A, Aduse-Opoku J, Curtis MA, Hughes FJ, Belibasakis GN.

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Osteoprotegerin/TNFRSF11B ELISA

Mouse Osteoprotegerin/TNFRSF11B DuoSet ELISA

Mouse Osteoprotegerin/TNFRSF11B DuoSet ELISA from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Mouse Osteoprotegerin/TNFRSF11B DuoSet ELISA from R&D Systems, a Bio-Techne Brand

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