Human MICA DuoSet ELISA DY1300 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman MICA DuoSet ELISA
CompanyR&D Systems, a Bio-Techne Brand
Price $890.00Supplier Page View Company Product Page
Catalog NumberDY1300
NCBI Gene AliasesMIC-A, PERB11.1
NCBI Full Gene NameMHC class I polypeptide-related sequence A
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
Detection TargetMICA
ReactivityHuman
Detection Range62.50 - 4000 pg/mL
ApplicationsELISA
Quantity1 Kit (for 15 Plates)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) The role of selected NKG2DLs such as MICA, MICB and ULBP4 as potential markers in multiple sclerosisScientific reportsJuly 29, 2025Pogoda-Wesołowska A, Świątek A, Synowiec A, Zdanowski R, Stachura I, Szewczuk A, Pietras E, Stępień A.CSF samples, the following ELISA kits were used: EH479RB (Life Technologies Corporation, CA, USA), DY1300 and DY1599 (R&D Systems, Minneapolis, MN, USA), respectively. All studies were performed according to

(2) Soluble MHC class I chain-related protein A is a specific biomarker for the early detection of graft-versus-host diseaseFront Med (Lausanne)June 5, 2025Alexander Kupis, Nina Buchtele, Philipp Wohlfarth, Werner Rabitsch, Katarina D. Kovacevic Miljevic, Marion Mussbacher, Bernd Jilma, Christian Schoergenhofer(REAADS vWF Antigen ELISA, K034-001 kits, DiaPharma, West Chester, OH, USA; Human MICA DuoSet ELISA, DY1300 kits, R&D Systems, Minneapolis, MN, USA). CRP and VWF were measured as established parameters of systemic

(3) Macrophage-derived pro-inflammatory cytokines augment the cytotoxicity of cytokine-induced killer cells by strengthening the NKG2D pathway in multiple myelomaScientific reportsMay 14, 2025Chen P, Chen Y, Wang Y, Sharma A, Veronika LK, Weiher H, Maria AG, Schmidt-Wolf IGH.respectively. For cytokines measurement, supernatants were 10 folds diluted prior to the use. The shed MICA (#DY1300) and MICB (#DY1599) ELISA kits were purchased from R&D System (Minneapolis, MN, USA), and TNF-α (#88-7346-22),

(4) Novel and potent MICA/B antibody is therapeutically effective in KRAS LKB1 mutant lung cancer modelsJ Immunother CancerJanuary 6, 2025Ryan R Kowash, Manoj Sabnani, Laura T Gray, Qing Deng, Nusrat U A Saleh, Luc Girard, Yujiro Naito, Kentaro Masahiro, John D Minna, David E Gerber, et al.Supernatants were harvested and MICA and MICB levels were quantified via sandwich ELISA (R&D Systems, DY1300 and DY1599). Isolation of NK cells from donor PBMCs (peripheral blood mononuclear cells) Peripheral

(5) A novel and potent MICA/B antibody is therapeutically effective in KRAS LKB1 mutant lung cancer modelsbioRxiv : the preprint server for biologyJuly 31, 2024Kowash RR, Sabnani M, Gray LT, Deng Q, Girard L, Naito Y, Masuhiro K, Minna JD, Gerber DE, Koyama S, et al.

(6) Soluble NKG2DLs Are Elevated in Breast Cancer Patients and Associate with Disease OutcomeInternational journal of molecular sciencesApril 8, 2024Seller A, Tegeler CM, Mauermann J, Schreiber T, Hagelstein I, Liebel K, Koch A, Heitmann JS, Greiner SM, Hayn C, et al.

(7) Synergistic integration of histone deacetylase inhibitors apparently enhances the cytokine-induced killer cell efficiency in multiple myeloma via the NKG2D pathwayClin Transl ImmunologyMarch 25, 2024Jingjing Pu, Amit Sharma, Ting Liu, Jian Hou, Ingo Gh Schmidt-Wolfsupernatant was harvested and the level of soluble MICA was determined using MICA Elisa kit (Duoset DY1300 and DY008, R&D Systems, Inc. Minneapolis, MN, USA) following the manufacturer's instruction. Absorbance

(8) Functional MICA Variants Are Differentially Associated with Immune-Mediated Inflammatory DiseasesInternational journal of molecular sciencesMarch 6, 2024Wang CM, Tan KP, Wu YJ, Zheng JW, Wu J, Chen JY.

(9) Clearing soluble MIC reverses the impaired function of natural killer cells from patients with multiple myelomaJournal for ImmunoTherapy of CancerJanuary 8, 2024Sojeong Kim, Haerim Chung, Jeong-Eun Kwak, Yu Ri Kim, Chung Hyun Park, Yeonhee Kim, June-Won Cheong, Jennifer Wu, Eui-Cheol Shin, Hyunsoo Cho, et al.in plasma or supernatant was measured by DuoSet ELISA (R&D Systems, Minnesota, USA) for human MICA (DY1300), MICB (DY1599), ULBP1 (DY1380), ULPB2 (DY1298) and ULBP3 (DY1517-05), using the procedures recommended

(10) A chimeric antigen receptor uniquely recognizing MICA/B stress proteins provides an effective approach to target solid tumorsMedJuly 14, 2023Goulding J, Yeh WI, Hancock B, Blum R, Xu T, Yang BH, Chang CW, Groff B, Avramis E, Pribadi M, et al.UFC903024). Total soluble MICA/B from concentrates were quantified by ELISA (Ab59569, Abcam, R&D Systems, DY1300 and DY1599). Following staining optimization, recombinant NKG2D-mFc protein (Acro Biosystems, NKD-H5259)

(11) MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cellsJournal for immunotherapy of cancerFebruary 1, 2023Morimoto Y, Yamashita N, Daimon T, Hirose H, Yamano S, Haratake N, Ishikawa S, Bhattacharya A, Fushimi A, Ahmad R, et al.by ELISA Supernatants from cells cultured for 3 days were analyzed using Human MICA DuoSet ELISA (DY1300, R&D Systems) and Human MICB DuoSet ELISA (DY1599, R&D Systems). Isolation and characterization of

(12) SARS-CoV-2 escapes direct NK cell killing through Nsp1-mediated downregulation of ligands for NKG2DCell reportsDecember 27, 2022Lee MJ, Leong MW, Rustagi A, Beck A, Zeng L, Holmes S, Qi LS, Blish CA.

(13) Bronchoalveolar lavage fluid reveals factors contributing to the efficacy of PD-1 blockade in lung cancerJCI insightMay 9, 2022Masuhiro K, Tamiya M, Fujimoto K, Koyama S, Naito Y, Osa A, Hirai T, Suzuki H, Okamoto N, Shiroyama T, et al.

(14) Cord blood and amniotic membrane extract eye drop preparations display immune-suppressive and regenerative propertiesScientific reportsJuly 2, 2021Samarkanova D, Cox S, Hernandez D, Rodriguez L, Pérez ML, Madrigal A, Vilarrodona A, Querol S, Casaroli-Marano RP.donor means of three replicate test samples. The presence of INF-γ and soluble NKG2D ligands MICA/B (DY1300/DY1599) and ULBP1 (DY1380) was tested in CBED and AMED. IFN-γ (DY285) was detected in PBMC PMA and ionomycin

(15) MICA*019 Allele and Soluble MICA as Biomarkers for Ankylosing Spondylitis in TaiwaneseJournal of personalized medicineJune 16, 2021Wang CM, Tan KP, Jan Wu YJ, Lin JC, Zheng JW, Yu AL, Wu JM, Chen JY.

(16) Increase of Antitumoral Effects of Cytokine-Induced Killer Cells by Antibody-Mediated Inhibition of MICA SheddingCancersJuly 7, 2020Wu X, Zhang Y, Li Y, Schmidt-Wolf IGH.supernatant was harvested and the level of soluble MICA was determined using MICA Elisa kit (Duoset DY1300 and DY008, R&D Systems, Inc. Minneapolis, MN, USA) following the manufacturer’s instruction. 4.9. Statistical

(17) Cord Blood Platelet Rich Plasma Derivatives for Clinical Applications in Non-transfusion MedicineFrontiers in ImmunologyMay 27, 2020Dinara Samarkanova, Steven Cox, Diana Hernandez, Luciano Rodriguez, Ricardo P. Casaroli-Marano, Alejandro Madrigal, Sergio QuerolSupplementary Figure S1. Soluble NKG2DL and IFN-γ Detection and Quantification Assays Soluble MICA/B (DY1300/DY1599) and ULBP1 (DY1380) were quantified in CB-PRP derivatives directly and IFN-γ (DY285) was measured

(18) Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to agingJCI insightJune 11, 2019Muñoz DP, Yannone SM, Daemen A, Sun Y, Vakar-Lopez F, Kawahara M, Freund AM, Rodier F, Wu JD, Desprez PY, et al.antibodies, inhibitors, and recombinant proteins ELISA kits were used to measure MICA (R&D Systems, DY1300), MICB (R&D Systems, DY1599), MICA/B (R&D Systems, DY990), MMP3 (R&D Systems, DMP300), TNF-α (R&D Systems, DTA00C),

(19) Race-associated expression of MHC class I polypeptide-related sequence A (MICA) in prostate cancerExperimental and molecular pathologyJune 1, 2019Sakiyama MJ, Espinoza I, Reddy A, de Carlo F, Kumar A, Levenson AS, Bae S, Zhou X, Claudio PP, Lewin J, et al.

(20) Histone Deacetylase Inhibitor Modulates NKG2D Receptor Expression and Memory Phenotype of Human Gamma/Delta T Cells Upon Interaction With Tumor CellsFrontiers in immunologyJanuary 1, 2019Bhat J, Dubin S, Dananberg A, Quabius ES, Fritsch J, Dowds CM, Saxena A, Chitadze G, Lettau M, Kabelitz D.Scientific) were used for coating with antibodies to quantitate soluble MICA, MICB, ULBP-1, ULBP-2 (#DY1300, #DY1599, #DY1380, #DY1298, respectively; all from R&D Systems) by ELISA according to manufacturer's

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MICA ELISA

Human MICA DuoSet ELISA

Human MICA DuoSet ELISA from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Human MICA DuoSet ELISA from R&D Systems, a Bio-Techne Brand

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