Mouse Osteopontin DuoSet ELISA DY441 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse Osteopontin DuoSet ELISA
CompanyR&D Systems, a Bio-Techne Brand
Price $890.00Supplier Page View Company Product Page
Catalog NumberDY441
Quantity1 Kit (for 15 Plates)
ApplicationsELISA
ReactivityMouse
Detection TargetOsteopontin/OPN
Detection Range15.60 - 1000 pg/mL
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date
NCBI Full Gene Namesecreted phosphoprotein 1
NCBI Gene Aliases2AR, Apl-1, BNSP, BSPI, Bsp, ETA-1, Eta, OP, Opn, Opnl, Ric, Spp-1

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Targeting EFEMP1 enhances chondrogenesis and inhibits hypertrophic differentiation in a spontaneous osteoarthritis mouse modelJ Mol Med (Berl)March 18, 2026Yuhan Chang, Pei-Heng Jiang, Yung-Heng Hsu, Yu-Chih Lin, Chih-Chien Hu, Yi-Zhen Li, Steve Wen-Neng Ueng, Mei-Feng Chen(ab83369; Abcam). Additionally, OPN concentration was measured using the Mouse Osteopontin DuoSet ELISA (DY441; R&D Systems), with results normalized to total protein concentration using the BCA Protein Assay Kit

(2) Calcitriol and Tacalcitol Modulate Th17 Differentiation Through Osteopontin Receptors: Age-Dependent Insights from a Mouse Breast Cancer ModelImmunotargets TherAugust 23, 2025Aleksandra Strzykalska-Augustyniak, Mateusz Psurski, Honorata Zachary, Beata Filip-Psurska, Dagmara Kłopotowska, Magdalena Milczarek, Marta Świtalska, Martyna Stachowicz-Suhs, Natalia Łabędź, Aleksandra Ziemblicka, et al.Name,Catalog Number,Tested Material,Manufacturer OPN,DY441,Plasma,"R&D Systems, Minneapolis, MN, USA" OPN,DY441,"R&D Systems, Minneapolis, MN, USA",Supernatants

(3) Calcitriol and Tacalcitol Modulate Th17 Differentiation Through Osteopontin Receptors: Age-Dependent Insights from a Mouse Breast Cancer ModelImmunotargets TherAugust 23, 2025Aleksandra Strzykalska-Augustyniak, Mateusz Psurski, Honorata Zachary, Beata Filip-Psurska, Dagmara Kłopotowska, Magdalena Milczarek, Marta Świtalska, Martyna Stachowicz-Suhs, Natalia Łabędź, Aleksandra Ziemblicka, et al.Name,Catalog Number,Tested Material,Manufacturer OPN,DY441,Plasma,"R&D Systems, Minneapolis, MN, USA" OPN,DY441,"R&D Systems, Minneapolis, MN, USA",Supernatants

(4) Intercellular communication between hepatic stellate cells and myofibroblasts mediated by osteopontin and FGF18 promotes liver fibrosisiScienceJuly 18, 2025Takao Seki, Sachiko Komazawa-Sakon, Takashi Nishina, Tetuo Mikami, Hideo Yagita, Katsuhide Okunishi, Minoru Tanaka, Yuichi Tsuchiya, Hiroyasu Nakanolevels in HSC culture supernatants were measured using the Mouse/Rat Osteopontin DuoSet ELISA Kit (DY441, R&D Systems) according to the manufacturer’s protocol. Briefly, ELISA plates were coated with capture

(5) Mesenchymal stromal cells reduce inflammation and improve lung function in a mouse model of cystic fibrosis lung diseaseScientific reportsDecember 28, 2024Doherty DF, Roets LE, Dougan CM, Brown RR, Hawthorne IJ, O'Kane C, Krasnodembskaya AD, Mall MA, Taggart CC, Weldon S.

(6) Intracellular Osteopontin Promotes the Release of TNFα by Mast Cells to Restrain Neuroendocrine Prostate CancerCancer Immunol ResJune 12, 2024Roberta Sulsenti, Giuseppina B. Scialpi, Barbara Frossi, Laura Botti, Renata Ferri, Irene Tripodi, Annamaria Piva, Sabina Sangaletti, Davide Pernici, Valeria Cancila, et al.(BioOptica). ELISA OPN in culture supernatants was detected using the DuoSet ELISA Kit (R&D System, cat. n. DY441) according to the manufacturer’s protocol. Optical densities were determined on a microplate reader

(7) Protocol for the isolation of mouse senescence-associated CD4(+) T cells using flow cytometry and functional assaysSTAR protocolsSeptember 15, 2023Fukushima Y, Minato N, Hattori M.Factor Staining Buffer Set,eBioscience,Cat# 00-5523-00 Mouse Osteopontin DuoSet ELISA,R&D systems,Cat# DY441 ,, Experimental models: Organisms/strains,Experimental models: Organisms/strains,Experimental models:

(8) Intracellular osteopontin protects from autoimmunity-driven lymphoma development inhibiting TLR9-MYD88-STAT3 signalingMolecular cancerDecember 12, 2022Rizzello C, Cancila V, Sangaletti S, Botti L, Ratti C, Milani M, Dugo M, Bertoni F, Tripodo C, Chiodoni C, et al.min. Supernatants (sera) were stored at -20 °C. The DuoSet kit for mouse Osteopontin (R&D System, #DY441) was used to test the amount of secreted OPN, following manufacturer’s instructions. Evaluation of

(9) cis interaction of CD153 with TCR/CD3 is crucial for the pathogenic activation of senescence-associated T cellsCell reportsSeptember 20, 2022Fukushima Y, Sakamoto K, Matsuda M, Yoshikai Y, Yagita H, Kitamura D, Chihara M, Minato N, Hattori M.

(10) Identification of kidney injury released circulating osteopontin as causal agent of respiratory failureScience advancesFebruary 25, 2022Khamissi FZ, Ning L, Kefaloyianni E, Dun H, Arthanarisami A, Keller A, Atkinson JJ, Li W, Wong B, Dietmann S, et al.measured in human or mouse serum samples using enzyme-linked immunosorbent assay (ELISA) kits (DY1433, DY441, and DY1817, respectively, all from R&D Systems) as per the manufacturer’s instructions. Serum dilutions

(11) WDR5-H3K4me3 epigenetic axis regulates OPN expression to compensate PD-L1 function to promote pancreatic cancer immune escapeJournal for immunotherapy of cancerJuly 1, 2021Lu C, Liu Z, Klement JD, Yang D, Merting AD, Poschel D, Albers T, Waller JL, Shi H, Liu K.supernatant and mouse serum was analyzed for OPN protein level using the mouse OPN ELISA kit (Cat # DY441, R&D System) according to the manufacturer’s instructions. WDR5 therapy and anti-PD-1 immunotherapy The

(12) STAT6-Dependent Exacerbation of House Dust Mite-Induced Allergic Airway Disease in Mice by Multi-Walled Carbon NanotubesNanoImpactApril 1, 2021Ihrie MD, Duke KS, Shipkowski KA, You DJ, Lee HY, Taylor-Just AJ, Bonner JC.

(13) ADAM8 affects glioblastoma progression by regulating osteopontin-mediated angiogenesisBiological chemistryJanuary 27, 2021Li Y, Guo S, Zhao K, Conrad C, Driescher C, Rothbart V, Schlomann U, Guerreiro H, Bopp MH, König A, et al.determined by ELISA kits for human OPN (R&D systems, DY1433, Southampton, UK) and mouse OPN (R&D Systems, DY441) according to the manufacturer’s instructions. 205 Statistical analysis Unpaired two-tailed Student’s

(14) Lipoteichoic Acid Accelerates Bone Healing by Enhancing Osteoblast Differentiation and Inhibiting Osteoclast Activation in a Mouse Model of Femoral DefectsInternational journal of molecular sciencesAugust 3, 2020Hu CC, Chang CH, Hsiao YM, Chang Y, Wu YY, Ueng SWN, Chen MF.Enzyme-Linked Immunosorbent Assay (ELISA) Osteopontin levels were measured using a Mouse Osteopontin DuoSet ELISA Kit (DY441, R&D Systems, Minneapolis, MA, USA), according to the manufacturer’s protocol. Thresholds for intra-

(15) The CD153 vaccine is a senotherapeutic option for preventing the accumulation of senescent T cells in miceNature communicationsMay 18, 2020Yoshida S, Nakagami H, Hayashi H, Ikeda Y, Sun J, Tenma A, Tomioka H, Kawano T, Shimamura M, Morishita R, et al.

(16) OC-STAMP promotes osteoclast fusion for pathogenic bone resorption in periodontitis via up-regulation of permissive fusogen CD9FASEB journal : official publication of the Federation of American Societies for Experimental BiologyJuly 1, 2018Ishii T, Ruiz-Torruella M, Ikeda A, Shindo S, Movila A, Mawardi H, Albassam A, Kayal RA, Al-Dharrab AA, Egashira K, et al.

(17) STAT1-dependent and -independent pulmonary allergic and fibrogenic responses in mice after exposure to tangled versus rod-like multi-walled carbon nanotubesParticle and Fibre ToxicologyDecember 1, 2017Katherine S. Duke, Alexia J. Taylor-Just, Mark D. Ihrie, Kelly A. Shipkowski, Elizabeth A. Thompson, Erinn C. Dandley, Gregory N. Parsons, James C. Bonnerkits (R & D Systems, Inc., Minneapolis, MN) specific for mouse TGF-β1 (DY1679), Osteopontin (OPN) (DY441), and IL-1β (DY401). Serum IgE was assayed using a BD Pharmingen ELISA kit (557,079, San Jose, CA).

(18) Physiologic Thymic Involution Underlies Age-Dependent Accumulation of Senescence-Associated CD4⁺ T CellsJournal of immunology (Baltimore, Md. : 1950)July 1, 2017Sato K, Kato A, Sekai M, Hamazaki Y, Minato N.difference. Cytokine assay OPN in the culture supernatants was assessed by with ELISA kits (cat. no. DY441; R&D Systems, Minneapolis, MN). Results Aged mouse environment is highly permissive for spontaneous proliferation

(19) Contribution of engineered nanomaterials physicochemical properties to mast cell degranulationScientific reportsMarch 6, 2017Johnson MM, Mendoza R, Raghavendra AJ, Podila R, Brown JM.

(20) Co-option of Liver Vessels and Not Sprouting Angiogenesis Drives Acquired Sorafenib Resistance in Hepatocellular CarcinomaJournal of the National Cancer InstituteAugust 1, 2016Elizabeth A. Kuczynski, Melissa Yin, Avinoam Bar-Zion, Christina R. Lee, Henriett Butz, Shan Man, Frances Daley, Peter B. Vermeulen, George M. Yousef, F. Stuart Foster, et al.enzyme-linked immunosorbent assay (ELISA) kits: mouse (m)VEGF-A (MMV00), human (h)VEGF-A (DVE00), mOPN (DY441), mSDF-1 (DY460), mG-CSF (DY414), mAng2 (MANG20), mPDGF-BB (MBB00), mPDGF-AB (MHD00), hbFGF (DFB50;

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Mouse Osteopontin DuoSet ELISA

Osteopontin/OPN ELISA

Mouse Osteopontin DuoSet ELISA from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Mouse Osteopontin DuoSet ELISA from R&D Systems, a Bio-Techne Brand

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