Fig 1: Aged VCD-treated mice display increased inflammatory signatures in plasma.Multiplex MSD chemiluminescence-based assay for eight proinflammatory cytokines at peri-Of and POST-OF time points (A-H). Plasma levels of IL-6 (A), TNF-α (B), and CXCL1 (D) were significantly increased in POST-OF mice compared to PERI-OF control mice of the same age. Plasma levels of IL-5 (C) were significantly increased in POST-OF mice compared to PERI- vehicle treated mice. Circulating cytokine levels for IL-10 (E), IFN-γ (F), IL-2 (G), and IL-1β (H) were not significant across groups. Graphs represent Mean ± SEM. Kruskal-Wallis test corrected for multiple comparisons was used to compare the means across the four groups. *p<0.05, **p<0.01.