P450-Glo CYP1A2 Assay V8771 from Promega

Product Specs
ItemP450-Glo CYP1A2 Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV8771
Detection TargetCYP1A2
Sample TypeRecombinant and native protein, microsomal fractions
Quantity10ml
MethodLuminescent

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Prioritisation of co-formulants and plant protection products for non-dietary risk assessment using NAMsArchives of ToxicologyJuly 2, 2025Alkiviadis Stagkos-Georgiadis, Bright Baffour-Duah, Tewes Tralau, Denise BlochP450-Glo™ CYP2C19 and CYP1A2 Screening Systems obtained from Promega (Cat.#V8882, Cat.#V4880, Cat.#V8772, Cat.#V4770, Madison, Wisconsin, USA), negative control membranes, Luciferin-H EGE (CYP2C19) and Luciferin-ME

(2) Synthesis of mono Cytochrome P450 in a modified CHO-CPR cell-free protein production platformScientific ReportsJanuary 13, 2024Jan Felix Knauer, Christian Schulz, Anne Zemella, Doreen A. Wüstenhagen, Ruben Magnus Walter, Jan-Heiner Küpper, Stefan KubickWisconsin, USA) were used. CYP1A2 activity was detected by Methoxy-Luciferin (Luciferin-ME) turnover (V8772), CYP2B6 was detected by Dimethoxybutyl-Luciferin (Luciferin-2B6) turnover (V8321) and CYP3A4 was detected

(3) Quantitative chemometric phenotyping of three-dimensional liver organoids by Raman spectral imagingCell Rep MethodsApril 24, 2023LaLone V, Aizenshtadt A, Goertz J, Skottvoll FS, Mota MB, You J, Zhao X, Berg HE, Stokowiec J, Yu M, et al.measured using P450-Glo™ Assay with Luciferin-IPA (catalogue no. V9001) and Luciferin-1A2 (catalogue no. V8771) correspondingly (Promega, Sweden). For the induction of CYP3A4 activity, organoids were treated with

(4) A Microfluidic Device for Long‐Term Maintenance of Organotypic Liver CulturesAdvanced Materials TechnologiesJanuary 24, 2023José M. de Hoyos de Hoyos‐Vega, Hye Jin Hong, Kevin Loutherback, Gulnaz Stybayeva, Alexander Revzinfrom Bethyl Lab and Cell Biolabs, respectively. P450-Glo CYP1A2 induction/inhibition assay system (V8771) was purchased from Promega. Sylgard 184 poly(dimethylsiloxane) (PDMS) kit (2065622) was purchased from

(5) Dimethyl fumarate reduces hepatocyte senescence following paracetamol exposureiScienceJune 25, 2021Meseguer-Ripolles J, Lucendo-Villarin B, Tucker C, Ferreira-Gonzalez S, Homer N, Wang Y, Starkey Lewis PJ, M Toledo E, Mellado-Gomez E, Simpson J, et al.Buffer,Agilent,Cat#5067- 5580 miRNeasy mini Kit,Qiagen,Cat#217004 P450-Glo CYP1A2 Assay and Screening System,Promega,Cat#V8771 P450-Glo CYP3A4 Assay and Screening System,Promega,Cat# V8801 QuantiTect Reverse Transcription Kit,Qiagen,Cat#205311 QuantSeq

(6) Protocol for automated production of human stem cell derived liver spheresSTAR protocolsJune 18, 2021Meseguer-Ripolles J, Kasarinaite A, Lucendo-Villarin B, Hay DC.Diagnostics,Cat#1190 ELISA Kit AFP,Alpha Diagnostics,Cat#500 P450-Glo CYP1A2 Assay and Screening System,Promega,Cat#V8771 ,, Experimental models: cell lines,Experimental models: cell lines,Experimental models: cell lines ,, Human embryonic stem cell

(7) Functional cytochrome P450 1A enzymes are induced in mouse and human islets following pollutant exposureDiabetologiaJanuary 1, 2020Muna Ibrahim, Erin M. MacFarlane, Geronimo Matteo, Myriam P. Hoyeck, Kayleigh R. C. Rick, Salar Farokhi, Catherine M. Copley, Shannon O’Dwyer, Jennifer E. Bruinhuman islets using the P450-Glo CYP1A1 Assay (#V8752; Promega, Madison, WI, USA) and CYP1A2 Assay (#V8772, Promega). All assays were performed in 96-well white-walled plates with clear bottoms (#655098; Greiner

(8) Differentiation Therapy by Epigenetic Reconditioning Exerts Antitumor Effects on Liver Cancer CellsMolecular TherapyJuly 1, 2018Luc Gailhouste, Lee Chuen Liew, Ken Yasukawa, Izuho Hatada, Yasuhito Tanaka, Hitoshi Nakagama, Takahiro OchiyaActivity The drug-metabolizing enzyme activities were determined with the P450-Glo CYP1A2 Assay (#V8772) and the P450-Glo CYP3A4 Assay (#V9002) from Promega, as recommended by the manufacturer. The CYP1A2

(9) Effect of an ethanol extract of Descurainia sophia seeds on Phase I and II drug metabolizing enzymes and P-glycoprotein activity in vitroBMC Complementary and Alternative MedicineDecember 18, 2015Jin-Mu Yi, Young Ah Kim, You Jin Lee, Ok-Sun Bang, No Soo Kiminhibitors Types,DMEs,Suppliers,Cat #,Suppliers,Cat #,Chemicals,Suppliers,Codes Ph I,CYP1A2,Promega,V8772,Invitrogen,P2792,α-naphthoflavone,Sigma-Aldrich,N5757 ,CYP2C8,Promega,V8782,Invitrogen,PV6138,Montelukast,Cayman,10008318 ,CYP2C9,Promega,V8792,Invitrogen,P2378,Sulfaphenazole,Sigma-Aldrich,S0758 ,CYP2C19,Promega,V8882,Invitrogen,P2864,Ticlopidine,Sigma-Aldrich,T6654 ,CYP2D6,Promega,V8892,Invitrogen,P2283,Quinidine,Sigma-Aldrich,Q3625 ,CYP3A4,Promega,V8802,Invitrogen,P2858,Ketoconazole,Sigma-Aldrich,K1003 Ph

(10) Defined plant extracts can protect human cells against combined xenobiotic effectsJournal of Occupational Medicine and ToxicologyDecember 1, 2011Céline Gasnier, Claire Laurant, Cécile Decroix-Laporte, Robin Mesnage, Emilie Clair, Carine Travert, Gilles-Eric SéraliniFrance). The cytochrome P450 CYP1A2 and CYP3A4 activities were quantified by the P450 Glo™ assays (V8771, Batch 271384 and V8801, Batch 277348, respectively; Promega, France). Each 2X Cytochrome P450/KPO 4

(11) Upregulation of CYP 450s expression of immortalized hepatocyte-like cells derived from mesenchymal stem cells by enzyme inducersBMC BiotechnologyDecember 1, 2011Khanit Sa-ngiamsuntorn, Adisak Wongkajornsilp, Kanda Kasetsinsombat, Sunisa Duangsa-ard, Lalana Nuntakarn, Suparerk Borwornpinyo, Pravit Akarasereenont, Somchai Limsrichamrern, Suradej HongengMetabolism was assessed based on luciferase activity using the P450-glo 1A1, 1A2, 2C9 and 3A4 assay (V8751, V8771, V8791, V9001; Promega, WI). After 3-d incubation period, cells were incubated with IMDM supplemented

(12) Culture of Mouse Embryonic Stem Cells with Serum but without Exogenous Growth Factors Is Sufficient to Generate Functional Hepatocyte-Like CellsPLoS ONEAugust 2, 2011Karen Pauwelyn, Philip Roelandt, Tineke Notelaers, Pau Sancho-Bru, Johan Fevery, Catherine M. Verfaillieactivity Cytochrome 1a2 activity was detected by using the non-lytic method of P450-Glo™ Assay (Promega V8901 and V8771). Induction of Cyp1a2 was performed by incubation 500 µM phenobarbital, as per manufacturer's protocol. Supporting

(13) In vivo liver regeneration potential of human induced pluripotent stem cells from diverse originsScience translational medicineMay 11, 2011Liu H, Kim Y, Sharkis S, Marchionni L, Jang YY.saline (PBS). Cytochrome P450 assay CYP activity was assessed using the pGlo kit (Promega, CYP1A2-V8771, CYP3A4-V8901, CYP2D6-V8891, CYP2C19-V8881, and CYP2C9-V8791) according to the manufacturer's instruction

(14) Generation of endoderm-derived human induced pluripotent stem cells from primary hepatocytesHepatologyAugust 12, 2010Hua Liu, Zhaohui Ye, Yong-Hak Kim, Saul Sharkis, Yoon-Young JangLaboratories). Cytochrome P450 Assay CYP1A2 and CYP3A4 activity was assessed using the pGlo kit (catalog numbers V8771, V8901; Promega) according to manufacturer’s instruction for nonlytic CYP450 activity estimation. hHiPS

(15) Human embryonic and rat adult stem cells with primitive endoderm-like phenotype can be fated to definitive endoderm, and finally hepatocyte-like cellsPloS oneAugust 11, 2010Roelandt P, Pauwelyn KA, Sancho-Bru P, Subramanian K, Bose B, Ordovas L, Vanuytsel K, Geraerts M, Firpo M, De Vos R, et al.P450 subtype activity was detected by using the non-lytic method of P450-Glo™ Assay (Promega V8901 and V8771). Induction of CYP3A4 was performed by incubation with 500 µM phenobarbital, induction of Cyp1a2 by

(16) Differentiation of rat multipotent adult progenitor cells to functional hepatocyte-like cells by mimicking embryonic liver developmentNature protocolsJuly 1, 2010Roelandt P, Sancho-Bru P, Pauwelyn K, Verfaillie C.(H3PO4) (Fluka, cat. no. 79626), Step 22, option F only • P450-Glo CYP1A2 Assay (Promega, cat. no. V8771), Step 22, option D only • QuantiChrom Urea Assay Kit (BioAssay Systems, cat. no. DIUR-500), Step 22,

(17) Directed differentiation of murine-induced pluripotent stem cells to functional hepatocyte-like cellsJournal of HepatologyJune 9, 2010Pau Sancho-Bru, Philip Roelandt, Nidhi Narain, Karen Pauwelyn, Tineke Notelaers, Takeshi Shimizu, Michael Ott, Catherine Verfaillie

(18) Generation of functional human hepatic endoderm from human induced pluripotent stem cellsHepatologyJanuary 1, 2010Gareth J. Sullivan et al.described [5]. Cytochrome p450 Assay CYP1A2 and CYP3A4 activity was assessed using the pGlo kit (Cat nos. V8771, V8901 Promega) according to manufacturer's instruction for non-lytic CYP450 activity estimation. CYP

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P450-Glo CYP1A2 Assay

P450-Glo CYP1A2 Assay from Promega

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P450-Glo CYP1A2 Assay from Promega

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