P450-Glo CYP1A1 Assay V8752 from Promega

Product Specs
ItemP450-Glo CYP1A1 Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV8752
Detection TargetCYP1A1
MethodLuminescent
Quantity50ml
Sample TypeRecombinant and native protein, microsomal fractions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Fluvastatin suppresses breast cancer initiation and progression via targeting CYP4Z1Communications BiologyJanuary 12, 2026Huilong Li, Ying Chen, Wanjin Shi, Zheng Miao, Yu Lu, Xuedan Han, Haitao Chen, Yunnan Zhang, Miaomiao Niu, Shengtao Xu, et al.CYP4Z1 protein solution. Reaction mixtures containing 12 μL protein solution, 0.5 μL fluorescein-CEE (V8752, Promega, Madison, WI, USA), and inhibitor cocktail were preincubated at 37 °C for 10 min in 96-well

(2) A bypass gateway from cholesterol to sex steroid biosynthesis circumnavigates CYP17A1Nat CommunDecember 4, 2025Ziqi Zhu, Yoon-Mi Chung, Mohammad Alyamani, Yijing Dai, Kevin D. McCarty, Evan Roberts, Sunita Sinha, Jianneng Li, Xiuxiu Li, Emad M. Gad, et al.CYP1A1 overexpression were measured 3 days after transfection using P450-Glo CYP1A1 Assay kit (Promega V8751, Wisconsin, U.S.A.). RNA-seq analysis To investigate the hormonal activity of DHC, we first starved

(3) Fluidic Programmable Gravi-maze Array for High Throughput Multiorgan Drug TestingbioRxivJune 27, 2025Henry C. Wong, Chris J. Collins, Joshna A. Jude Jose, Isha N. Bhakta, Andrew J. Collins, Gunjan Katara, Johar Kohana, Harpreet S. Saluja, John Collinswas assessed using a luciferin-based chemiluminescent assay (Promega, P450-Glo™ CYP1A2 Assay, Cat. #V8751), following chemical induction of the organ models. Standard curves and controls were included for all

(4) Astrocytes Respond to Environment-Relevant Doses TCDD in a Specific Manner Which Is Different from the Endogenous AhR Ligand (FICZ)Environ Health (Wash)April 18, 2025Rui Sha, Yangsheng Chen, Tong Xu, Guanglei Yang, Li Xu, Heidi Qunhui Xie, Bin Zhaoobtained from Sigma (St. Louis, MO). FICZ and DEMO were dissolved by DMSO. P450-Glo CYP1A1 Assay System (V8752) was purchased from Promega (Madison, WI). Cxcl2 ELISA Kit (ab155463) and Ccl7 ELISA Kit (ab205571)

(5) Continuous Production of Highly Functional Vascularized Hepatobiliary Organoids from Human Pluripotent Stem Cells using a Scalable Microfluidic PlatformAdvanced Functional MaterialsDecember 1, 2023Saeed Abbasalizadeh, Sahab Babaee, Reza Kowsari‐Esfahan, Zahra Mazidi, Yichao Shi, Jake Wainer, Joaquim M. S. Cabral, Robert Langer, Giovanni Traverso, Hossein Baharvand(Luciferin-IPA) – V9002 – Promega), CYP2B6 (P450-Glo CYP2B6 – V8322 – Promega), and CYP1A1 (P450-Glo CYP1A1 – V8752 – Promega) by incubating them with different inducers. Undifferentiated hPSC, primary hepatocytes and

(6) Butyrate acts through HDAC inhibition to enhance aryl hydrocarbon receptor activation by gut microbiota-derived ligandsGut microbesJanuary 1, 2022Modoux M, Rolhion N, Lefevre JH, Oeuvray C, Nádvorník P, Illes P, Emond P, Parc Y, Mani S, Dvorak Z, et al.recombinant CYP1A1 (Sigma Aldrich; Cat#C3735) was assessed using P450-Glo™ CYP1A1 Assay (Promega; Cat#V8751) and NADPH regeneration system (Promega; Cat#V9510). Reactions were performed in duplicate with butyrate

(7) Evaluation of Placentation and the Role of the Aryl Hydrocarbon Receptor Pathway in a Rat Model of Dioxin ExposureEnvironmental health perspectivesNovember 1, 2021Iqbal K, Pierce SH, Kozai K, Dhakal P, Scott RL, Roby KF, Vyhlidal CA, Soares MJ.analyses. CYP1A1 Enzyme Activity Assay CYP1A1 activity was measured using the P450-Glo CYP1A1 assay kit (V8751; Promega), according to the manufacturer’s instructions. Liver tissue (n=3–5 per group WT or AHR Null

(8) Long-term metabolic consequences of acute dioxin exposure differ between male and female miceScientific ReportsDecember 1, 2020Myriam P. Hoyeck, Hannah Blair, Muna Ibrahim, Shivani Solanki, Mariam Elsawy, Arina Prakash, Kayleigh R. C. Rick, Geronimo Matteo, Shannon O’Dwyer, Jennifer E. Bruinwas measured in isolated mouse islets (50 islets per well) using the Promega P450-Glo CYP1A1 Assay (#V8752) in 96-well white-walled plates (Greiner Bio-One, # 655098) using the lytic method, as described by

(9) Differential Monocyte Actuation in a Three‐Organ Functional Innate Immune System‐on‐a‐ChipAdvanced ScienceJuly 1, 2020Trevor Sasserath et al.times with 1× PBS and incubated with the 1A1 specific substrate, Luciferin‐CEE (120 × 10−6 m) (Promega, V8752) in phenol red‐free 1× Dulbecco's modified Eagle medium (DMEM) (Thermo Fisher Scientific, A1443001)

(10) Functional cytochrome P450 1A enzymes are induced in mouse and human islets following pollutant exposureDiabetologiaJanuary 1, 2020Muna Ibrahim, Erin M. MacFarlane, Geronimo Matteo, Myriam P. Hoyeck, Kayleigh R. C. Rick, Salar Farokhi, Catherine M. Copley, Shannon O’Dwyer, Jennifer E. BruinMIN6, βTC-6, INS1, α-TC1, α-TC3) and isolated mouse and human islets using the P450-Glo CYP1A1 Assay (#V8752; Promega, Madison, WI, USA) and CYP1A2 Assay (#V8772, Promega). All assays were performed in 96-well

(11) Nano-fibre Integrated Microcapsules: A Nano-in-Micro Platform for 3D Cell CultureScientific ReportsDecember 1, 2019Shalil Khanal, Shanta R. Bhattarai, Jagannathan Sankar, Ramji K. Bhandari, Jeffrey M. Macdonald, Narayan Bhattaraienzyme activities were measured by P450-Glo CYP1A1 assay kit (Promega Co., Madison, WI, USA, catalog No: V8751) as described previously89 with some modification. Briefly, CYP1A1 activity was induced by incubating

(12) Generation of fully functional hepatocyte-like organoids from human induced pluripotent stem cells mixed with Endothelial CellsScientific reportsJune 20, 2019Pettinato G, Lehoux S, Ramanathan R, Salem MM, He LX, Muse O, Flaumenhaft R, Thompson MT, Rouse EA, Cummings RD, et al.Madison, WI). Different P450 enzymes activity were tested, in particular the CYP1A1 (P450-Glo CYP1A1 – V8752 – Promega, Madison, WI), CYP1A2 (P450-Glo CYP1A2 Induction/Inhibition – V8422 – Promega, Madison, WI),

(13) Enhancement of the gut barrier integrity by a microbial metabolite through the Nrf2 pathwayNature communicationsJanuary 9, 2019Singh R, Chandrashekharappa S, Bodduluri SR, Baby BV, Hegde B, Kotla NG, Hiwale AA, Saiyed T, Patel P, Vijay-Kumar M, et al.residual drugs, and fresh medium containing Cyp1A1 substrate (as per protocol provided with kit Cat.# V8751; Promega) for 3 h. After incubation, 25 µl of culture medium was removed from each well and transferred

(14) A pumpless body-on-a-chip model using a primary culture of human intestinal cells and a 3D culture of liver cellsLab on a ChipJune 12, 2018Huanhuan Joyce Chen, Paula Miller, Michael L. ShulerP450 enzyme activity P450 enzyme activity was measured by using Promega Glo assay kits (Promega, WI #V8752 for CYP1A1 and #V9002 for CYP3A4). Initially, the P450 “Induced” Groups were refreshed with medium containing

(15) Development of Hepatocyte-like Cell Derived from Human Induced Pluripotent Stem cell as a Host for Clinically Isolated Hepatitis C VirusCurrent protocols in stem cell biologyAugust 14, 2017Sa-Ngiamsuntorn K, Hongeng S, Wongkajornsilp A.cat. no. O104)Phenobarbital (Sigma, cat. no. P5178)EthanolP450‐Glo 1A1, 2B6, 2C9 and 3A4 assay kits (V8751, V8321, V8791, V9001; Promega,)Williams' Medium E (Thermo Fisher Scientific, cat. no. 12551032)Luciferin‐CEE,

(16) Indoleamine 2,3-dioxygenase 1 (IDO1) inhibitors activate the aryl hydrocarbon receptorToxicology and Applied PharmacologyMay 1, 2017Benjamin J. Moyer, Itzel Y. Rojas, Iain A. Murray, Seokwon Lee, Haley F. Hazlett, Gary H. Perdew, Craig R. Tomlinsontreatments, 1MLT and 1MDT were dissolved in 50% DMSO. The P450-Glo™ Assay kit specific for CYP1A1 (catalog# V8752) was purchased from Promega (Madison, WI). Cell Culture Author Manuscript Murine Hepa-1c1c7 (Hankinson

(17) A tryptophan derivative, ITE, enhances liver cell metabolic functions in vitroInternational Journal of Molecular MedicineJanuary 1, 2017Xiaoqian Zhang, Juan Lu, Bin He, Lingling Tang, Xiaoli Liu, Danhua Zhu, Hongcui Cao, Yingjie Wang, Lanjuan Liactivity was performed with a P450-Glo CYP1A2 assay (V8422), a CYP3A4 assay (V9002), a CYP1A1 assay (V8752) and a CYP1B1 assay (V8762) (all from Promega, Madison, WI, USA). In brief, the cells were incubated

(18) Design and demonstration of a pumpless 14 compartment microphysiological systemBiotechnology and bioengineeringOctober 1, 2016Miller PG, Shuler ML.determined using Promega Glo assays (Promega Corp., Madison, WI, USA, catalog #V9002 for CYP3A4 and catalog #V8752 for CYP1A1) following the manufacturer’s instructions, see Supplemental Materials for details. The us results

(19) A robust model of natural hepatitis C infection using hepatocyte-like cells derived from human induced pluripotent stem cells as a long-term hostVirology journalApril 5, 2016Sa-Ngiamsuntorn K, Wongkajornsilp A, Phanthong P, Borwornpinyo S, Kitiyanant N, Chantratita W, Hongeng S.activities were assessed based on luciferase activity using the P450-glo 1A1, 2B6, 2C9 and 3A4 assays (V8751, V8321, V8791, V9001; Promega, WI). After 3-d incubation period, cells were incubated with Williams’

(20) Impact Assessment of Cigarette Smoke Exposure on Organotypic Bronchial Epithelial Tissue Cultures: A Comparison of Mono-Culture and Coculture Model Containing FibroblastsToxicological SciencesSeptember 1, 2015Anita R. Iskandar et al.activity Combined activity of CYP1A1/CYP1B1 was determined using the nonlytic P450-Glo assay (Cat. No. V8752; Promega, Madison, Wisconsin) according to the manufacturer’s recommendations. Briefly, the luminogenic

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P450-Glo CYP1A1 Assay

P450-Glo CYP1A1 Assay from Promega

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P450-Glo CYP1A1 Assay from Promega

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