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Beta-Glo Assay System
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Beta-Glo Assay System
Beta-Glo Assay System
Beta-Glo Assay System from Promega
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Citations:
(31)
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Beta-Glo Assay System
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Description
The Beta-Glo Assay System is a homogeneous, luminescence-based method for quantitating beta-galactosidase expression in mammalian cells
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Beta-Glo Assay System
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
E4720
Detection Target
beta-galactosidase
Method
Luminescent
Sample Type
Mammalian cells
Quantity
10ml
Detection
luminescent
Molecular Weight
~65kDa
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (31)
(1)
Simulated Microgravity Reprograms Host Cell Architecture and Mitochondrial Function, Altering Toxoplasma gondii Growth
FASEB Bioadv
July 21, 2026
Musfika Tasnim Shirin, Takafumi Yamada, Daisuke Kondoh, Hiroki Bochimoto, Kentaro Kato
RH‐2F
strain
of T. gondii for 48 h. The parasite was quantified using the
Beta‐Glo Assay System
(cat.
E4740
,
Promega
) according to the manufacturer's instructions. Briefly, an equal volume of β‐Glo reagent was
(2)
Helminth proteins recapitulate key structural motifs of MIF to facilitate immunomodulation of host receptors
iScience
July 17, 2026
Jacob A. Orkwis, Ramu Manjula, Elias J. Lolis
hexahydrate,Sigma-Aldrich,Cat# N9389Lot# 0000272169Source# SLCM5201
Beta-Glo Assay System
,
Promega
,Cat#
E4720
,, Deposited data,Deposited data,Deposited data ,, cDNA: hMIF,UniProtKB,P14174 cDNA: naMIF1,NCBI,XP_013307584.1XM_013452130.2ETN85357.1KAK6753325.1 cDNA:
(3)
NF-κB-Driven HIV-1 Gene Expression in Human Cells Is Independent of Poly(ADP-ribose) polymerase-1 Function
bioRxiv
April 11, 2026
Denisse A. Gutierrez, Manuel Llano
were measured three days later. β-galactosidase was measured with the
Beta-Glo assay system
(
Promega
,
E4720
). Luciferase levels were normalized to β-galactosidase activity to control for
transfection efficiency
. The
(4)
Wnt target IQGAP3 promotes Wnt signaling via disrupting Axin1-CK1α interaction
Oncogene
October 20, 2025
Muhammad Bakhait Rahmat, Aashiq Hussain, Yu Xuan Teh, Bibek Dutta, Sumedha Pundrik, Dennis Kappei, Yoshiaki Ito
aliquoted into white, flat bottom, 96-well plate (Costar) for treatment with either Beta-Glo® (
Promega
, #
E4720
) or
Luciferase Assay Reagent
II/LARII (
Promega
, #
E1910
) reagent.
Real-Time Polymerase chain reaction
(5)
PYR1 Biosensor-Driven Genome-Wide CRISPR Screens for Improved Monoterpene Production in Kluyveromyces marxianus
ACS Synthetic Biology
August 15, 2025
Nicholas R. Robertson, Chase Lenert-Mondou, Alison C. Leonard, Aida Tafrishi, Stephanie Carrera, Sangcheon Lee, Yuna Aguilar, Leonardo Sanchez Zamora, Trinity Nguyen, Jesús Beltrán,
et al.
determine the dose-dependent response of the PYR1Geraniol receptor, the Beta-Glo
assay
(
Promega
, CAT#:
E4720
) was performed. 50 mL cultures of SD-Trp,-Leu were inoculated with the
biosensor
-containing
yeast
(MaV99+pAD+pBD,
(6)
Phosphorylation-activated G protein signaling stabilizes TCP14 and JAZ3 to repress JA signaling and enhance plant immunity
Molecular plant
July 7, 2025
Jia H, Hewitt N, Jordá L, Abramyan TM, Tolliver J, Jones JL, Nomura K, Yang J, He SY, Tropsha A,
et al
.
and 10g/L raffinose for 2 days, and then follows the instruction of the
Beta-Glo Assay System
(Cat#
E4720
,
Promega
) to quantitate the β-galactosidase expression in
yeast
cells for different mating pairs. The
(7)
Phloroglucinol Enhances Anagen Signaling and Alleviates H2O2-Induced Oxidative Stress in Human Dermal Papilla Cells
Journal of Microbiology and Biotechnology
April 28, 2024
Seokmuk Park, Ye Jin Lim, Hee Su Kim, Hee-Jae Shin, Ji-Seon Kim, Jae Nam Lee, Jae Ho Lee, Seunghee Bae
using a microplate reader.
β-Galactosidase
activity was assessed utilizing the
Beta-Glo Assay System
(#
E4720
;
Promega
), and relative luciferase activity was normalized to β-galactosidase activity. Conditioned
(8)
In vitro hair growth-promoting effects of araliadiol via the p38/PPAR-γ signaling pathway in human hair follicle stem cells and dermal papilla cells
Frontiers in pharmacology
January 1, 2024
Park S, Park HW, Seo DB, Yoo DS, Bae S.
using the microplate reader.
β-Galactosidase
activity was measured using the
Beta-Glo Assay System
(#
E4720
;
Promega
), and relative luciferase activity was normalized to β-galactosidase activity. 2.9 RNA extraction
(9)
The in vivo functional significance of PUF hub partnerships in C. elegans germline stem cells
Development
May 1, 2023
Ahlan S. Ferdous, Stephany J. Costa Dos Santos, Charlotte R. Kanzler, Heaji Shin, Brian H. Carrick, Sarah L. Crittenden, Marvin Wickens, Judith Kimble
with -Leu-Trp using the Beta-Glow®
Assay
System following commercially available protocols (
Promega
,
E4720
). In short,
yeast
cultures were grown to mid-log phase, diluted to the same optical density (0.1), and
(10)
Functional significance of PUF partnerships in C. elegans germline stem cells
bioRxiv
February 22, 2023
Ahlan S. Ferdous, Stephany J. Costa Dos Santos, Charlotte R. Kanzler, Heaji Shin, Brian H. Carrick, Sarah L. Crittenden, Marvin Wickens, Judith Kimble
with -Leu-Trp using the Beta-Glow®
Assay
System following commercially available protocols (
Promega
#
E4720
). In short,
yeast
cultures were grown to mid-log phase, diluted to the same optical density (0.1), and
(11)
The human CNOT1-CNOT10-CNOT11 complex forms a structural platform for protein-protein interactions
Cell reports
January 31, 2023
Mauxion F, Basquin J, Ozgur S, Rame M, Albrecht J, Schäfer I, Séraphin B, Conti E.
commercial
assays
,,
Effectene Transfection Reagent
,
Qiagen
,Cat#
301427
Beta-Glo Assay System
,
Promega
,Cat#
E4720
,, Deposited data,Deposited data,Deposited data ,, CNOT1N-
CNOT10
-CNOT11,This paper,PDB: 8BFI CNOT11C,This
(12)
Polypyrimidine-Tract-Binding Protein Isoforms Differentially Regulate the Hepatitis C Virus Internal Ribosome Entry Site
Viruses
December 20, 2022
Jenniffer Angulo, C. Joaquín Cáceres, Nataly Contreras, Leandro Fernández-García, Nathalie Chamond, Melissa Ameur, Bruno Sargueil, Marcelo López-Lastra
each sample was estimated by measuring β-galactosidase activity using the Beta-GloTM
Assay
System #
E4720
,
Promega Corporation
) according to the manufacturer’s instructions on a Sirius Single Tube Luminometer
(13)
Genetic manipulation of the human gut bacterium Eggerthella lenta reveals a widespread family of transcriptional regulators
Nature communications
December 9, 2022
Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, Turnbaugh PJ, Balskus EP.
other lacZ reporters was performed in 96-well plates (Corning) using
Beta-Glo® Assay System
(
Promega
,
E4740
). For cumate-inducible lacZ constructs, saturated cultures harbouring either pXD70CT3 or pXD70CT5 were
(14)
Remodeling of the Tumor Microenvironment Through PAK4 Inhibition Sensitizes Tumors to Immune Checkpoint Blockade
Cancer Res Commun
October 19, 2022
Gabriel Abril-Rodriguez, Davis Y. Torrejon, Daniel Karin, Katie M. Campbell, Egmidio Medina, Justin D. Saco, Mildred Galvez, Ameya S. Champhekar, Ivan Perez-Garcilazo, Ignacio Baselga-Carretero,
et al.
Bright-Glo Luciferase Assay System
(
Promega
, catalog no. PR-
E2610
) and the
Beta-Glo Assay System
(
Promega
, catalog no. PR-
E4720
). Protein-level Quantification CXCL10 expression was measured by
RT-PCR
following the manufacturer's
(15)
Remodeling of the tumor microenvironment through PAK4 inhibition sensitizes tumors to immune checkpoint blockade
Cancer Res Commun
October 1, 2022
Abril-Rodríguez G, Torrejon DY, Karin D, Campbell KM, Medina E, Saco JD, Galvez M, Champhekar AS, Perez-Garcilazo I, Baselga-Carretero I,
et al
.
Bright-Glo Luciferase Assay System
(
Promega
, catalog no. PR-
E2610
) and the
Beta-Glo Assay System
(
Promega
, catalog no. PR-
E4720
). Protein-level Quantification CXCL10 expression was measured by
RT-PCR
following the manufacturer's
(16)
A genetic compensatory mechanism regulated by Jun and Mef2d modulates the expression of distinct class IIa Hdacs to ensure peripheral nerve myelination and repair
eLife
January 25, 2022
Velasco-Aviles S, Patel N, Casillas-Bajo A, Frutos-Rincón L, Velasco E, Gallar J, Arthur-Farraj P, Gomez-Sanchez JA, Cabedo H.
kit,
Luciferase Assay System
,
Promega
,Cat#
E1500
, Commercial
assay
or kit,
Beta-Glo Assay System
,
Promega
,Cat#
E4720
, Commercial
assay
or kit,
Pierce BCA Protein Assay Kit
,
Thermo Scientific
,"Cat# 23,225", Commercial
(17)
Therapeutic downregulation of neuronal PAS domain 2 (Npas2) promotes surgical skin wound healing
eLife
January 18, 2022
Yoichiro Shibuya, Akishige Hokugo, Hiroko Okawa, Takeru Kondo, Daniel Khalil, Lixin Wang, Yvonne Roca, Adam Clements, Hodaka Sasaki, Ella Berry,
et al.
measure
Npas2-LacZ expression, β-galactosidase activity was measured using a Beta-Glo
Assay
System (
E4720
,
Promega
). The Npas2-LacZ expression data were uploaded to an online data analysis tool (CDD Vault,
(18)
Therapeutic downregulation of neuronal PAS domain 2 (Npas2) promotes surgical skin wound healing
Elife
January 18, 2022
Yoichiro Shibuya, Akishige Hokugo, Hiroko Okawa, Takeru Kondo, Daniel Khalil, Lixin Wang, Yvonne Roca, Adam Clements, Hodaka Sasaki, Ella Berry,
et al.
measure
Npas2-LacZ expression, β-galactosidase activity was measured using a Beta-Glo
Assay
System (
E4720
,
Promega
). The Npas2-LacZ expression data were uploaded to an online data analysis tool (CDD Vault,
(19)
The Sall2 transcription factor promotes cell migration regulating focal adhesion turnover and integrin β1 expression
Frontiers in cell and developmental biology
January 1, 2022
Riffo E, Palma M, Hepp MI, Benítez-Riquelme D, Torres VA, Castro AF, Pincheira R.
for luciferase and β-Gal activity using the manufacturer’s suggested protocols (
Promega
;
E1483
and
E4740
, respectively). Luminescent reporter activity was measured using a Luminometer (Victor3; Perkin- Elmer).
(20)
Biotransformation of rare earth oxide nanoparticles eliciting microbiota imbalance
Particle and Fibre Toxicology
December 1, 2021
Huizhen Zheng et al.
1,2-distearoyl-sn-glycero-3-phosphocholine (PC, 18:0/18:0, Mw: 790, No. 850365) was purchased from Avanti (USA).
Beta-Glo Assay System
(
E4720
) used for
membrane
permeability test was purchased from
Promega
(USA). 4′-(trimethylammonio) diphenylhexatriene
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