Description
The QBT™ Fatty Acid Uptake Assay is a single-step homogeneous fluorescent assay that provides real-time uptake kinetics and is ideally suited for high throughput screening applications in both 96-well and 384-well formats. The kit employs a BODIPY® -dodecanoic acid fluorescent fatty acid analog coupled with Molecular Devices proprietary† quench technology. The BODIPY label provides an ideal long chain fatty acid analog that behaves much like natural fatty acids: it becomes activated by acyl-CoA attachment; is incorporated into di-and triglycerides; and accumulates in intracellular lipid droplets. In addition, the BODIPY analog is a known substrate for fatty acid transporters since its uptake by adipocytes can be competed by non-labeled fatty acids. The elimination of radioactive compounds results in easier reagent handling, reduced disposal costs and eliminates safety risks associated with radiolabel assays.