ELISA MAX™ Standard Set Human IFN-γ 430101 from BioLegend

Product Specs
ItemELISA MAX™ Standard Set Human IFN-γ
CompanyBioLegend
Price $265.00Supplier Page View Company Product Page
Catalog Number430101
ApplicationsELISA
Detection Range15.6-2000 pg/ml
MethodELISA
Quantity5 Plates
NCBI Full Gene Nameinterferon gamma
NCBI Gene AliasesIFG, IFI, IMD69
ReactivityHuman
Detection TargetIFN-gamma
TypeELISA Kit

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Sequence and structural determinants of efficacious de novo chimeric antigen receptorsbioRxivDecember 13, 2025Arthur Chow, Hoyin Chu, Ruofan Li, Benan N. Nalbant, Abdul Vehab Dozic, Laura C. Kida, Caleb A. Lareauaccording to the manufacturer’s instructions. Kits and input of supernatant used: IFN-y (Biolegend, Cat. 430101), IL-2 (Biolegend, Cat. 431801), Granzyme B (Invitrogen, Cat. BMS2027–2), TNF-α (Biolegend, Cat. 430201). For

(2) IDR-induced CAR condensation improves the cytotoxicity of CAR-Ts against low-antigen cancersNature Chemical BiologySeptember 29, 2025Xinyan Zhang, Qian Xiao, Longhui Zeng, Fawzaan Hashmi, Kazuki Sato, Tobias Max Philipp Hartwich, Miranda Mansolf, Yang Yang-Hartwich, Xiaolei Sucytokine measurement using ELISA kits (IL-2 ELISA kit, BioLegend, 431801; IFNγ ELISA kit, BioLegend, 430101; TNF ELISA kit, BioLegend, 430204) or using a LEGENDplex human CD8/NK panel kit (741186), according

(3) Comparison of different T cell assays for the retrospective determination of SARS-CoV-2 infectionJournal of General VirologyDecember 20, 2024Benjamin Krishna, Marina Metaxaki, Marianne Perera, Mark Wills, Nyarie Sitholefor IL-2 and IFN-γ were performed using ELISA MAX Deluxe Sets from BioLegend (Cat numbers 431801 and 430101, respectively), following the manufacturer’s protocols. In short, plates were coated overnight in antibodies

(4) Exclusion of PD-1 from the immune synapse: A novel strategy to modulate T cell functionMol Ther OncolSeptember 19, 2024Hao LY, Lerrer S, Paiola M, Moore EK, Gartshteyn Y, Song R, Goeckeritz M, Black MJ, Bukhari S, Hu X, et al.IL-2 ELISA kit (catalog number 431801, BioLegend) and human interferon (IFN)-gamma kit (catalog number 430101, BioLegend) were used according to the manufacturer protocols. Flow cytometry The binding of full-length

(5) Oncolytic alphavirus replicons mediated recruitment and activation of T cellsiScienceMarch 15, 2024Darshak K. Bhatt, Saskia L. Meuleman, Baukje Nynke Hoogeboom, Toos Daemenperformed using the supernatants to quantify the amount of secreted CXCL10 (439904, BioLegend), IFN-ƴ (430101, BioLegend) and Flt3L (DFK00, RnD Systems) using kits according to the suppliers’ protocol. Monolayer

(6) Atovaquone enhances antitumor efficacy of TCR-T therapy by augmentation of ROS-induced ferroptosis in hepatocellular carcinomaCancer Immunology, ImmunotherapyFebruary 13, 2024Anan Chen, Zhiwu Yu, Na Ma, Xinyu Lu, Yajing Zhang, Weikang Xu, Yiyue Wang, Jiayi Xie, Yuqi Qin, Guoheng Mo, et al.IFN-γ and TNF-α were respectively determined using IL-2 ELISA kit (431803, BioLegend), IFN-γ ELISA kit (430101, BioLegend), and TNF-α ELISA kit (430201, BioLegend) according to the manufacturer’s instructions. Absorbance

(7) Single-Cell Transcriptomics Reveals the Heterogeneity of the Immune Landscape of IDH-Wild-Type High-Grade GliomasCancer immunology researchFebruary 2, 2024Ran X, Zheng J, Chen L, Xia Z, Wang Y, Sun C, Guo C, Lin P, Liu F, Wang C, et al.T cells cocultured with tumor cells. IFNγ secretion was quantified with ELISA kits (BioLegend, cat. no. 430101) and Immuno Clear Standard Modules (Thermo, cat. no. 468667), following the manufacturer's protocols. Statistical

(8) Safety and efficacy of a novel anti-CD19 chimeric antigen receptor T cell product targeting a membrane-proximal domain of CD19 with fast on- and off-rates against non-Hodgkin lymphoma: a first-in-human studyMol CancerDecember 9, 2023Yunlin Zhang, Ruchi P. Patel, Ki Hyun Kim, Hyungwoo Cho, Jae-Cheol Jo, Seong Hyun Jeong, Sung Yong Oh, Yoon Seok Choi, Sung Hyun Kim, Ji Hyun Lee, et al.performed using ELISA MAX standard set human IL-2 (BioLegend; Cat# 431801) or human IFN-γ (BioLegend; Cat# 430101 and BD Biosciences; Cat# 555142) kits. Cell avidity measurement using z-Movi CAR T cell binding avidity

(9) IDR-induced CAR condensation improves the cytotoxicity of CAR-Ts against low-antigen cancersbioRxivNovember 2, 2023Xinyan Zhang, Qian Xiao, Longhui Zeng, Fawzaan Hashmi, Xiaolei Sucytokine measurement using ELISA kits (IL2 ELISA kit, BioLegend #431801; IFNγ ELISA kit, BioLegend #430101; TNFα ELISA kit, BioLegend # 430204) or using LEGENDplex™ Human CD8/NK Panel Kit (Cat# 741186), according

(10) Sézary syndrome originates from heavily mutated hematopoietic progenitorsBlood AdvancesSeptember 26, 2023Carly M. Harro, Kimberly B. Sprenger, Ricardo A. Chaurio, John J. Powers, Patrick Innamarato, Carmen M. Anadon, Yumeng Zhang, Subir Biswas, Gunjan Mandal, Jessica A. Mine, et al.enzyme-linked immunosorbent assay were performed using ELISA MAX standard set kits for human interferon γ (Biolegend, 430101) and IL-4 (Biolegend, 430301) following manufacturer’s protocol to assess cytokine concentration. 10×

(11) Granulocyte-Macrophage Colony-Stimulating Factor Influence on Soluble and Membrane-Bound ICOS in Combination with Immune Checkpoint BlockadeCancer immunology researchAugust 3, 2023Li X, Li J, Zheng Y, Lee SJ, Zhou J, Giobbie-Hurder A, Butterfield LH, Dranoff G, Hodi FS.and IFNγ in cell culture supernatants, IL12 p40 (430701), IL12 p70 (431701), TNFα (430201), and IFNγ (430101) ELISA kits purchased from BioLegend were used according to the manufacturer's protocols, respectively.

(12) SLAMF6 compartmentalization enhances T cell functionsLife science allianceFebruary 1, 2023Gartshteyn Y, Askanase AD, Song R, Bukhari S, Dragovich M, Adam K, Mor A.software. The ELISA To determine the concentration of secreted proteins after stimulation, human IFN-γ (#430101; BioLegend) and human IL-2 (#431801; BioLegend) detection kits were used. To quantify anti-CD45 and

(13) Serum 25-hydroxycholesterol levels are increased in patients with coronavirus disease 2019Journal of clinical lipidologyJanuary 1, 2023Asano T, Wakabayashi T, Kondo Y, Okada K, Yamamuro D, Koga Y, Oka K, Sakurai M, Sawayama N, Takahashi M, et al.cytokine concentrations using ELISA MAX Standard Sets (BioLegend; human IL-6, #430501; human IFN-γ, #430101), according to the manufacturer's instructions. Cell Culture Experiments Blood was collected from

(14) PD-1/LAG-3 bispecific antibody potentiates T cell activation and increases antitumor efficacyFrontiers in ImmunologyNovember 28, 2022Ning Shi, Yangyihua Zhou, Yujun Liu, Ran Zhang, Xingjun Jiang, Caiping Ren, Xiang Gao, Longlong Luo480130) and ELISA MAX™ Standard Set Human kit IFN-γ) were purchased from Biolegend corporation (Cat. No.: 430101); Cytotoxicity Detection Kit PLUS (LDH) were purchased from Roche(Cat. No. 04744934001); Simple PlexTM

(15) Antibody-dependent cellular cytotoxicity-null effector developed using mammalian and plant GlycoDelete platformScientific reportsNovember 8, 2022Kang CE, Lee S, Ahn T, Seo DH, Ko BJ, Jung M, Lee J, Kim JY, Kim WT.the plate at 1500 rpm for 20 min. The levels of IL-2 and INF-γ were measure by ELIZA (#431081 and #430101, respectively, Biolegend). HEK 293 T cell (ATCC), breast cancer cell lines MCF7 (PD-L1 (−), Korean Cell

(16) Screening and Identification of a Novel Anti-Siglec-15 Human Antibody 3F1 and Relevant Antitumor ActivityMolecular pharmacologySeptember 1, 2022Wu J, Peng J, Zhou Y, Zhang R, Wang Z, Hu N, Zhang D, Quan G, Wu Y, Feng J, et al.

(17) Size-dependent activation of CAR-T cellsScience immunologyAugust 5, 2022Xiao Q, Zhang X, Tu L, Cao J, Hinrichs CS, Su X.cytokine measurement using ELISA kits (IL2 ELISA kit, BioLegend #431801; IFNγ ELISA kit, BioLegend #430101; TNFα ELISA kit, BioLegend # 430204) according to the manufacturer’s instruction. Flow cytometry To

(18) Olfactory Receptor OR2H1 Is an Effective Target for CAR T Cells in Human Epithelial TumorsMolecular cancer therapeuticsJuly 5, 2022Martin AL, Anadon CM, Biswas S, Mine JA, Handley KF, Payne KK, Mandal G, Chaurio RA, Powers JJ, Sprenger KB, et al.supernatant was harvested and used for IFNγ detection using ELISA MAX™ Deluxe Set Human IFNγ (Biolegend 430101) and absorbance was measured at 450nm with Gen5 Microplate reader and Image Software (Biotek). Animals NOD-SCID-gamma

(19) Generation of effector Vγ9Vδ2 T cells and evaluation of their response to phosphoantigen-loaded cellsSTAR protocolsJune 17, 2022Hsiao CC, Wiemer AJ.,Miltenyi Biotec,Cat#130-092-892 MS columns,Miltenyi Biotec,Cat#130-042-201 ELISA MAX™ Standard Set Human IFN-γ,BioLegend,Cat#430101 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, K562 cells

(20) Ligand-induced interactions between butyrophilin 2A1 and 3A1 internal domains in the HMBPP receptor complexCell Chemical BiologyJune 1, 2022Chia-Hung Christine Christine Hsiao, Khiem Nguyen, Yiming Jin, Olga Vinogradova, Andrew J. Wiemerassays "TCRγ/δ+T Cell Iso Kit, human",Miltenyi,Cat#130–092-892 ELISA MAX™ Standard Set Human IFN-γ,Biolegend,Cat#430101 Nano-BRET PPI starter kit (618 ligand probe and luciferin substrate),Promega,Cat#N1811 ,, Experimental models

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ELISA MAX™ Standard Set Human IFN-γ from BioLegend

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ELISA MAX™ Standard Set Human IFN-γ from BioLegend

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