WST-8 Assay Kit (Cell Proliferation) ab65475 from Abcam

Product Specs
ItemWST-8 Assay Kit (Cell Proliferation)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65475
Quantity500 Test, 2500 Test
MethodFuncS
Sample TypeAdherent cells, Suspension cells
Assay Time4h 0m

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Synergistic effects through targeting the PI3K and IGFR pathways in treating lung cancer carrying activation alterations along the PI3K pathwayTransl OncolApril 3, 2026Mohamed Abd El-Salam, Wu Chen, Yan Tang, Ting Rao, Xuejia Kang, Lifang Sun, Tiegang Han, Pengyu Chen, Matthew Mossanen, Fan Cheng, et al.or their combination for 24 hours and analyzed using the colorimetric WST-1 cytotoxicity assay (Cat# ab65475, Abcam). The combination index of the drugs was calculated using the Chou–Talalay method as described

(2) An elastin-like polymer targeting vascular endothelial growth factor receptor-1 reduces survival in serum-starved endothelial cellsBiochemical Engineering JournalJanuary 1, 2026M. Lisa Phipps, Antonietta M. Lillo, Demosthenes P. Morales, Miriam Hernandez-Romero, Leyma P. De Haro, Devin Close, Wynter A. Paiva, Emily K. Funsten, Andrew R. M. Bradbury, Jennifer S. Martinez, et al.(#140–09) was from PeproTech. Rabbit anti-human/mouse-VEGFR1 antibody (#ab2350) and WST-1 reagent (#ab65475) were from Abcam. Goat anti-rabbit-Alexa488 antibody (#A-11034) was from Invitrogen. Anti-SV5-PE antibody

(3) Pancreatic cancer cachexia is mediated by PTHrP-driven disruption of adipose de novo lipogenesisbioRxivJune 7, 2025Nikita Bhalerao, Yamini Ogoti, Jessica Peura, Calvin Johnson, Qingbo Chen, Ekaterina D. Korobkina, Faith N. Keller, Maximilian Wengyn, Robert J. Norgard, Claire Shamber, et al.were tested regularly for Mycoplasma. Cell proliferation assay The WST-1 cell proliferation assay (ab65475, Abcam) was performed per the manufacturer’s instructions. Briefly, 2.0 × 103 cells were plated on 96-well

(4) High-Throughput Transcriptomics Identifies Chemoresistance-Associated Gene Expression Signatures in Human AngiosarcomaInternational Journal of Molecular SciencesOctober 9, 2024Glenys Mai Shia Khor, Sara Haghani, Tiffany Rui En Tan, Elizabeth Chun Yong Lee, Bavani Kannan, Boon Yee Lim, Jing Yi Lee, Zexi Guo, Tun Kiat Ko, Jason Yongsheng Chanat concentrations of 10, 20, 50, and 100 ng/mL for 72 h. The Quick Cell Proliferation Assay Kit II (ab65475, Abcam, Cambridge, UK) was used to quantify the overall cell viability after treatment with the respective

(5) Translational Potential of Baicalein in Mitigating RSL3-Induced Ferroptosis in Fibroblasts: Implications for Therapeutic InterventionsInternational Journal of Medical SciencesMay 13, 2024Pao-Jen Kuo, Cheng-Shyuan Rau, Yi-Chan Wu, Chia-Wen Tsai, Chia-Jung Wu, Chia-Wei Lin, Ching-Hua HsiehTaiwan). CytoScan water-soluble tetrazolium salt (WST-1) Cell Proliferation Assay kit (catalog number ab65475) was purchased from Abcam (Cambridge, UK). Horseradish peroxidase (catalogue number: AP132P) was purchased

(6) Effects of moderate doses of ionizing radiation on experimental abdominal aortic aneurysmPloS oneJanuary 1, 2024Riazi G, Brizais C, Garali I, Al-Rifai R, Quelquejay H, Monceau V, Vares G, Ould-Boukhitine L, Aubeleau D, Gilain F, et al.proliferation Cell proliferation was measured using the WST-1 kit (Abcam, Quick Cell Proliferation Assay, ab65475). Cells were cultured in 96-well plates at a concentration of 35,000 cells per well, with 100 μL of

(7) Sphingosine-1-phosphate Treatment Improves Cryopreservation Efficiency in Human Mesenchymal Stem CellsLife (Basel)May 30, 2023Seong-Ju Oh, Chan-Hee Jo, Tae-Seok Kim, Chae-Yeon Hong, Sung-Lim Lee, Young-Hoon Kang, Gyu-Jin Rhoh. Cells were then treated with or without 10 μM S1P. After 1 h of treatment, WST-1 reagent (Abcam, #ab65475) was added to each well, and the cells were incubated for 2 h at 37 °C. The absorbance was measured

(8) p85α regulatory subunit isoform controls PI3-kinase and TRPC6 membrane translocationCell calciumMay 1, 2023Chaudhuri P, Putta P, Graham LM, Rosenbaum MA.proliferation was assessed using the Quick Cell Proliferation Kit II (Abcam, Boston, MA; Catalog No. ab65475), following the manufacturer’s protocol. Absorbance was measured at 450 nm using a Spectramax 190 microplate

(9) SH2 Domain-Containing Phosphatase-SHP2 Attenuates Fibrotic Responses through Negative Regulation of Mitochondrial Metabolism in Lung FibroblastsDiagnostics (Basel, Switzerland)March 18, 2023Karampitsakos T, Galaris A, Barbayianni I, DeIuliis G, Ahangari F, Sampsonas F, Sotiropoulou V, Aidinis V, Bennett AM, Herazo-Maya JD, et al.Abcam, Cambridge, UK (ab). 2.2. Proliferation Assay The Quick Cell Proliferation Assay Kit (Abcam-ab65473) was used to measure primary MLFs from SHP2D61G/+ and SHP2+/+ mice proliferation, as it was previously

(10) Low-density lipoprotein encapsulated thiosemicarbazone metal complexes is active targeting vehicle for breast, lung, and prostate cancersDrug DeliveryDecember 31, 2022Laila Jaragh-Alhadad, Mayada Samir, Terri J. Harford, Sadashiva Karnikin-vitro cytotoxicity assay was carried out based on the manufacturer’s protocol provided by WST-1 assay Kit (ab65473 Abcam, USA). Briefly, 5 × 104 cells/well/ml from the MCF7, A549, and C42 cell lines were seeded on 96-well

(11) Cancer targeted drug delivery using active low-density lipoprotein nanoparticles encapsulated pyrimidines heterocyclic anticancer agents as microtubule inhibitorsDrug DeliveryDecember 31, 2022Laila Jaragh-Alhadad, Haider Behbehani, Sadashiva Karnikin-vitro cytotoxicity assay was carried out based on the manufacturer's protocol provided by WST-1 assay Kit (ab65473 Abcam, USA). Briefly, 5 × 104 cell/well/ml from the breast and prostate cell lines were seeded on 96-well

(12) Acid external and internal environment exchange the Oreochromis niloticus tissue immune gene expression compared to the mouse macrophage polarization modelFrontiers in ImmunologySeptember 26, 2022Po-Kai Pan, Tsung-Meng Wu, Hsin-Yuan Tsai, I-Cheng Cho, Hsin-Wei Tseng, Tai-Du Lin, Fan-Hua Nan, Yu-Sheng Wuend of the incubation time, a cell counting kit-8 (CCK-8) (B34302, Bimake) and WST-1 detection kit (ab65473, Abcam) were used to examine cell survival and proliferation activity by measuring cellular dehydrogenase

(13) p53 as a unique target of action of cisplatin in acute leukaemia cellsJournal of Cellular and Molecular MedicineSeptember 1, 2022Sanjay Kumar, Paul B. Tchounwouculture medium for 48 h. After incubation, WST cell proliferation was performed by Abcam Company (Cat # ab65473) instruction manual. In brief, after cisplatin treatment, 10‐μl WST/ECS solution was added to each well

(14) Immunostimulatory activity of soybean hull polysaccharide on macrophagesExperimental and Therapeutic MedicineApril 12, 2022Mengxue Wang, Chenye Fu, Mingcong Zhang, Yuxian Zhang, Liang Caowere determined using the Griess reaction and water-soluble tetrazolium-1 (WST-1) assays (cat. no. ab65475; Abcam), respectively, according to the manufacturer's instructions. The optical density was measured

(15) Precision analysis of mutant U2AF1 activity reveals deployment of stress granules in myeloid malignanciesMolecular cellMarch 17, 2022Biancon G, Joshi P, Zimmer JT, Hunck T, Gao Y, Lessard MD, Courchaine E, Barentine AES, Machyna M, Botti V, et al.viability measurements were obtained by flow cytometry and by colorimetric WST-1 assay (Abcam, Cat #ab65475). For flow cytometry analysis, cells were stained with 1.5 μl 7-aminoactinomycin D (7-AAD) solution

(16) Nitro-Deficient Niclosamide Confers Reduced Genotoxicity and Retains Mitochondrial Uncoupling Activity for Cancer TherapyInternational journal of molecular sciencesSeptember 27, 2021Ngai TW, Elfar GA, Yeo P, Phua N, Hor JH, Chen S, Ho YS, Cheok CF.Cell Proliferation Assay Cell viability was measured using WST-1 Cell Proliferation Assay (Abcam, USA ab65473). A total of 1500 cells were seeded onto a 96-well plate and were seated for 24 h before drug treatment

(17) PTHrP Drives Pancreatic Cancer Growth and Metastasis and Reveals a New Therapeutic VulnerabilityCancer discoveryJuly 1, 2021Pitarresi JR, Norgard RJ, Chiarella AM, Suzuki K, Bakir B, Sahu V, Li J, Zhao J, Marchand B, Wengyn MD, et al.RNA isolated for downstream analysis. Cell viability assays WST-1 Cell Proliferation Assay (Abcam ab65475) was performed per the manufacturer’s instructions. Briefly, 2.0×103-5.0×103 cells were plated on 96-well

(18) Long noncoding RNA TINCR is a novel regulator of human bronchial epithelial cell differentiation statePhysiological reportsFebruary 1, 2021Omote N, Sakamoto K, Li Q, Schupp JC, Adams T, Ahangari F, Chioccioli M, DeIuliis G, Hashimoto N, Hasegawa Y, et al.density of 10,000 cells/well. Cells were analyzed 0, 24, and 72 h later by WST‐1 Assay Kit (cat. No ab65475, Abcam). 2.8 Immunofluorescence staining and confocal laser scanning microscopy Cultured NHBEs on

(19) Dietary magnesium supplementation improves lifespan in a mouse model of progeriaEMBO Molecular MedicineOctober 7, 2020Ricardo Villa‐Bellostatetrazolium salt which in cleavage to formazan by cellular mitochondria dehydrogenase, (WST‐1 Assay Kit, ab65475, Abcam). Water‐soluble WST‐1 was added to each well, and the absorbance was measured using a scanning

(20) Circulating extracellular vesicles from patients with breast cancer enhance migration and invasion via a Src‑dependent pathway in MDA‑MB‑231 breast cancer cellsMolecular medicine reportsSeptember 1, 2020Ramírez-Ricardo J, Leal-Orta E, Martínez-Baeza E, Ortiz-Mendoza C, Breton-Mora F, Herrera-Torres A, Elizalde-Acosta I, Cortes-Reynosa P, Thompson-Bonilla R, Perez Salazar E.treated for 48 h at 37°C with EV fractions (20,000 EVs/condition). Next, 10 µl WST-1 reagent (cat. no. ab65473; Abcam) was added to the cells in each well and microplates were incubated at 37°C for 2 h. The absorbance

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WST-8 Assay Kit (Cell Proliferation) from Abcam

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WST-8 Assay Kit (Cell Proliferation) from Abcam

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