Choline/Acetylcholine Assay Kit ab65345 from Abcam

Product Specs
ItemCholine/Acetylcholine Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65345
MethodFuncS
Quantity100 Test, 2000 Test
Sample TypeCell culture supernatant, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts
Assay Time0h 40m
SensitivityMore than 0.01 nmol/well
Detection Range0.01 - 5 nmol/well

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Abcam

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Long-term vagus nerve stimulation synergized with rapamycin elicits neuroimmune modulation to prolong skin allograft survivaliScienceApril 17, 2026Xinghua Meng, Zhan Fan, Zhenzhen Cai, Mingdong Xie, Jingping Su, Shiqi Luo, Hongyi Yang, Zhihong Zhangassays ,, Epinephrine/Norepinephrine ELISA Kit,Abnova,KA1877 Choline/Acetylcholine Assay Kit,Abcam,ab65345 ELISA MAX™ Deluxe Set Mouse IFN-γ,BioLegend,430804 ELISA MAX™ Deluxe Set Mouse TNF-α,BioLegend,430904 ELISA MAX™ Deluxe Set Mouse IL-4

(2) Two types of axonal muscarinic acetylcholine receptors mediate formation of saliva cocktail in the tick Ixodes ricinusNature CommunicationsJanuary 23, 2026Cáinà Nìng, James J. Valdés, Lourdes Mateos-Hernández, Sabine Rakotobe, Lianet Abuin-Denis, Nadia Haddad, Lívia Šofranková, Mirko Slovák, Khalid Boussaine, Alison Cartereau, et al.levels in tissue homogenates were quantified using the Choline/Acetylcholine Assay Kit (Abcam, Cat. No. ab65345) following the manufacturer’s instructions. Each pooled sample was analysed in duplicate on 96-well

(3) Ginseng acetylcholine from various ginsengs and its in vivo physiological effectJournal of Ginseng ResearchJanuary 19, 2026Rami Lee, Ji-Hun Kim, Jang-Uk Kim, Sung-Hee Hwang, Seung-Yeol Nahanalysis or enzyme-linked immunosorbent assay (ELISA, a colorimetric choline/acetylcholine assay kit (ab65345, Abcam, Cambridge, MA, UK). FPLC operation conditions are shown in Table 1. To obtain abundant fraction

(4) Stabilization of CXCL12 (SDF-1α) via Silk Fibroin Films Enhances Stem Cell Migration/Retention and Functional Recovery After StrokeRegenerative BiomaterialsDecember 12, 2025Amira Lekouaghet, Marta Sánchez-Díez, José Pérez-Rigueiro, Francisco J Rojo, Carmen Ramírez-Castillejo, Yolanda Ruiz-León, Fivos Panetsos, Gustavo V Guinea, Daniel González-Nietoat −80 °C, and concentrations quantified by ELISA (R&D Systems, Cat. MCX120 for SDF-1α; Abcam, Cat. ab65345 for ACh). In vivo studies All animal procedures were conducted in accordance with national ethical

(5) Metabolic costs and trade-offs of hypermetabolism in human motor neurons with ATP synthase deficiencyCommunications biologyDecember 11, 2025Torregrosa-Muñumer R, Turkia J, Ermiş R, Kvist J, Harjuhaahto S, Pennonen J, Hietakangas V, Ylikallio E, Tyynismaa H.kit) Total acetylcholine levels in day 36 motor neurons were measured using a fluorometric kit (Abcam, #Ab65345) by following the manufacturer’s instructions. To ensure an adequate number of cells for each measurement,

(6) Enteric neuronal Piezo1 maintains mechanical and immunological homeostasis by sensing forceCellMay 1, 2025Zili Xie, Lillian Rose, Jing Feng, Yonghui Zhao, Yisi Lu, Harry Kane, Timothy J. Hibberd, Xueming Hu, Zhen Wang, Kaikai Zang, et al.weighed, and added to Assay Buffer VI/Choline Assay Buffer from Choline/Acetylcholine Assay Kit (Abcam# ab65345) in Omni bead rupture tubes (VWR#. 10032-756) and homogenized. Samples were spun down at maximum speed

(7) Endogenous extraction yielded high quality sulforaphane from broccoli sprouts unveils potent antioxidant and anti-Alzheimer's activitiesHeliyonFebruary 28, 2025Shyam Kokkattunivarthil Uthaman, Wan Seok Kang, Ju-Young Park, Sunoh Kim, Duc Dat Le, Suk-Jung Oh, Karthik Murugesh, Laura Minju Oh, Mina Lee, Jin Woo Parkacetylcholine (ACh) and acetylcholinesterase (AChE) activity using specific assay kits (ACh assay kit: ab65345, AChE assay kit: ab138871, Abcam, Cambridge, UK). These assays utilize enzymatic reactions to quantify

(8) Cognitive-enhancing effect of Cordia dichotoma fruit on scopolamine-induced cognitive impairment in rats: metabolite profiling, in vivo, and in silico investigations†RSC AdvancesDecember 17, 2024Hagar M. Hussein, Mostafa A. Abdel Kawy, Basma M. Eltanany, Laura Pont, Fernando Benavente, Ahmed M. Fayez, Radwan Alnajjar, Ahmed A. Al-Karmalawy, Azza R. Abdelmonem, Engy Mohsenwere employed. AChE (U mg−1) and ACh (nmol mg−1) levels were measured in the tissue with assay kits ab65345 and ab138871 from Abcam (Cambridge, UK), respectively. NA (ng mg−1) and DA (pg mg−1) levels were determined

(9) Fermented Gold Kiwi Improves Gastrointestinal Motility and Functional Constipation: An Animal Study and Human Randomized Clinical TestNutrientsNovember 3, 2024Jihye Choi, Hwal Choi, Yuseong Jang, Hyeon-Gi Paik, Hyuck-Se Kwon, Seon Mi Shin, Jeung Seung Lee, Bumseok Kim, Jungkee KwonImmunSmol SAS, Pessac, France). Serum acetylcholine was measured using an acetylcholine assay kit (ab65345, Abcam, Cambridge, UK). All assays were conducted according to the manufacturer’s instructions. 2.5.

(10) MFSD7c functions as a transporter of choline at the blood-brain barrierCell researchMarch 1, 2024Nguyen XTA, Le TNU, Nguyen TQ, Thi Thuy Ha H, Artati A, Leong NCP, Nguyen DT, Lim PY, Susanto AV, Huang Q, et al.and acetylcholine from plasma, liver, and brain samples were measured by fluorometric assay (Abcam: ab65345). Tissues were homogenized in choline buffer. Choline and acetylcholine were assayed according to the

(11) VNS improves VSMC metabolism and arteriogenesis in infarcted hearts through m/n-AChR-Akt-SDF-1α in adult male ratsJournal of Molecular HistologyFebruary 1, 2024Xing-yuan Li, Jia-Qi Liu, Yan Wang, Yan Chen, Wen-hui Hu, Yan-xia Lv, Yan Wu, Jing Lv, Jun-ming Tang, Deying Kongsubsequent detection and analysis. ELISA for ACh detection According to the manufacturer’s protocol (ab65345, Abcam), ELISA for ACh in heart tissues and blood samples treated with the cholinesterase inhibitor

(12) Exploration of the mechanism underlying the therapeutic effect of electroacupuncture at chengshan acupoint on post-hemorrhoidectomy anal pain: Insights from the mAChRs/IP3-Ca(2+)-CaM signaling pathwayClinics (Sao Paulo, Brazil)January 1, 2024Song Y, Wang Y, Li M, Wang Y, Xu T.until analysis. The analysis of Ach levels was conducted using the EnzyChrom™ Acetylcholine Assay Kit (ab65345, Abcam, UK). Plasma samples underwent a precise extraction procedure using the provided extraction buffer,

(13) Vagus nerve stimulation-induced stromal cell-derived factor-l alpha participates in angiogenesis and repair of infarcted heartsESC heart failureDecember 1, 2023Wang Y, Liu Y, Li XY, Yao LY, Mbadhi M, Chen SJ, Lv YX, Bao X, Chen L, Chen SY, et al.inhibitor eserine (100 μM) after VNS using a commercial kit by following the manufacturer's protocol (ab65345, Abcam). ELISA for TNF‐α (900‐M73, PeproTech), IL‐6 (900‐M86, PeproTech) and IL‐1β (900‐M91, PeproTech)

(14) SARS-CoV-2 S1 protein causes brain inflammation by reducing intracerebral acetylcholine productioniScienceJune 16, 2023Naomi Oka, Kazuya Shimada, Azusa Ishii, Nobuyuki Kobayashi, Kazuhiro Kondo74116 PrimeScript RT Reagent Kit,Takara Bio,Cat# RR037A Choline/Acetylcholine Assay Kit,Abcam,Cat# ab65345 ,, Deposited data,Deposited data,Deposited data ,, Original Data,Mendeley Data,https://doi.org/10.17632/ch52rydwjh.1 ,, Experimental models

(15) Non-neuronal cholinergic system delays cardiac remodelling in type 1 diabetesHeliyonJune 1, 2023Munasinghe PE, Saw EL, Reily-Bell M, Tonkin D, Kakinuma Y, Fronius M, Katare R.levels were determined by HPLC [15] and using a commercially available Choline/Acetylcholine assay kit (ab65345, Abcam) [28]. For HPLC, the left ventricle was harvested and homogenized in 0.1 M perchloric acid containing

(16) Auditory or Audiovisual Stimulation Ameliorates Cognitive Impairment and Neuropathology in ApoE4 Knock-In MiceInternational Journal of Molecular SciencesJanuary 4, 2023Harry Jung, Yeonkyeong Lee, Sang-Hwa Lee, Jong-Hee Sohnprepared from the right hemisphere was measured using a colorimetric choline/acetylcholine assay kit (ab65345, Abcam, Cambridge, MA, UK) following the manufacturer’s guidelines. Samples and standards were reacted

(17) Acetylcholine deficit causes dysfunctional inhibitory control in an aging-dependent mannerScientific ReportsDecember 3, 2022Paul Rafael Sabandal, Erick Benjamin Saldes, Kyung-An Hanmeasure acetylcholine, we utilized a commercially available Choline/Acetylcholine assay kit (catalog # ab65345, Abcam, Cambridge, United Kingdom). Briefly, 50 frozen fly heads per condition (genotype and age) were

(18) Impact of Neurons on Patient-Derived Cardiomyocytes Using Organ-On-A-Chip and iPSC BiotechnologiesCellsNovember 25, 2022Albin A. Bernardin, Sarah Colombani, Antoine Rousselot, Virginie Andry, Yannick Goumon, Hélène Delanoë-Ayari, Côme Pasqualin, Bernard Brugg, Etienne D. Jacotot, Jean-Luc Pasquié, et al.released in cell medium was quantified using fluorometric kit (Acetylcholine, Abcam, Waltham, MA, USA, ab65345). The four neurotransmitters were quantified in supernatant of 14 days differentiated PC12 cells harvested

(19) Microbial regulation of intestinal motility provides resistance against helminth infectionMucosal immunologyJune 1, 2022Moyat M, Lebon L, Perdijk O, Wickramasinghe LC, Zaiss MM, Mosconi I, Volpe B, Guenat N, Shah K, Coakley G, et al.from either from the tissue or the lumen were processed using Choline/Acetylcholine Assay Kit (Abcam, ab65345). Lumen samples were collected by gently scraping the small intestine with a coverslip to recover content

(20) A cholinergic neuroskeletal interface promotes bone formation during postnatal growth and exerciseCell stem cellApril 7, 2022Gadomski S, Fielding C, García-García A, Korn C, Kapeni C, Ashraf S, Villadiego J, Toro RD, Domingues O, Skepper JN, et al.ACh measurements using the Choline/Acetylcholine Assay Kit, Fluorometric protocol (Abcam, Cat. No. ab65345). SCG culture supernatants were assessed using Mouse IL-6 ELISA kit (abcam, Cat. No. ab222503). ELISAs

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Choline/Acetylcholine Assay Kit from Abcam

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Choline/Acetylcholine Assay Kit from Abcam

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