Reagent Diluent Concentrate 2 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemReagent Diluent Concentrate 2
CompanyR&D Systems, a Bio-Techne Brand
Price $320.00Supplier Page View Company Product Page
Catalog NumberDY995
Quantity1 Pack
ApplicationsELISA
StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date

Specifications/Features

Available conjugates: Sizes Available: 1 Pack

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Safety and biodistribution of intrathecal administration of mesenchymal stem cells (MSCs) and neurotrophin-releasing nanoparticles in a porcine CSF-guided delivery model for amyotrophic lateral sclerosis (ALS) drug discoveryScientific ReportsFebruary 27, 2026Emilia Sinderewicz, Maria Dąbkowska, Anna Sarnowska, Joanna Staszkiewicz-Chodor, Dorota Mystkowska, Piotr Holak, Igor Drozd, Magdalena Chodkowska-Michalowska, Monika Rytel, Edyta Paczkowska, et al.using a commercially available enzyme-linked immunosorbent (ELISA) assay (DY992, DY990, DY994, DY999, DY995, WA126, DY006, DY268, R&D Systems). The temperature of the experiments was kept constant at 298 ± 0.1

(2) Improving a Plasma Biomarker Panel for Early Detection of Pancreatic Ductal Adenocarcinoma with Aminopeptidase N (ANPEP) and Polymeric Immunoglobulin Receptor (PIGR)Clinical Cancer ResearchFebruary 17, 2026Brianna M. Krusen, Phyllis A. Gimotty, Greg Donahue, Jacob E. Till, Melinda Yin, Erin E. Carlson, William R. Bamlet, Erica L. Carpenter, Shounak Majumder, Ann L. Oberg, et al.ANPEP samples were diluted in 1% BSA in PBS using R&D Systems® Reagent Diluent Concentrate 2 (Catalog # DY995) from DuoSet ELISA Ancillary Reagent Kit 2. Plates for all assays were read at 450 nm using Molecular Devices

(3) Improving a Plasma Biomarker Panel for Early Detection of Pancreatic Ductal Adenocarcinoma with Aminopeptidase N (ANPEP) and Polymeric immunoglobin receptor (PIGR)Clin Cancer ResFebruary 17, 2026Brianna M. Krusen, Phyllis A. Gimotty, Greg Donahue, Jacob E. Till, Melinda Yin, Erin E. Carlson, William R. Bamlet, Erica L. Carpenter, Shounak Majumder, Ann L. Oberg, et al.ANPEP samples were diluted in 1% BSA in PBS using R&D Systems® Reagent Diluent Concentrate 2 (Catalog # DY995) from DuoSet ELISA Ancillary Reagent Kit 2. Plates for all assays were read at 450 nm using Molecular Devices

(4) Modulation of Corticotropin-Releasing Hormone Receptor Expression During In Vitro Keratinocyte DifferentiationCurrent Issues in Molecular BiologyFebruary 14, 2026Carole-Anne Martins, Sara Lesink, Angéline Roux, Guillaume Collet, Richard Daniellou°C with PBS (P4474, Sigma-Aldrich, Saint-Louis, MO, USA) containing 1% bovine serum albumin (BSA) (DY995, R&D Systems, Minneapolis, MN, USA). Cells were then incubated with rabbit anti-hCRHR1 (ACR-050-200UL,

(5) Spatial proteomics reveals recombinant human laminin-111 restores adhesion signaling to laminin-α2–deficient muscleJCI InsightNovember 24, 2025Hailey J. Hermann, Ryan D. Wuebbles, Marisela Dagda, Axel Muñoz, Lauren L. Parker, Paula C. C Guzman, Lola T. Byrne, Steven A. Moore, Dean J. Burkinparaformaldehyde for 5 minutes (excluding p-JNK, HSP27, and HSP70) followed by blocking with 1% BSA (R&D Systems, DY995) or blocking buffer from the Mouse-on-Mouse Detection Kit (Vector Labs, BMK-2202) when using mouse primary

(6) Increased circulating heat shock protein Hsp70 serum levels as a potential biomarker in bronchial asthma patientsScientific ReportsNovember 18, 2025Ella Churyukina, Elena Oganesyan, Olga Ukhanova, Inga Kotieva, Marina Gulyan, Elena Koreeva, Ekaterina Portnyaga, Danila Bobkov, Stephanie E. Combs, Maxim Shevtsovwashing. The detection antibody (cat. no. 841681, R&D Systems, USA) was diluted in IC Diluent #1 (cat. no. DY995, R&D Systems, USA) at a concentration of 100 ng/ml, 100 µl were added per well and incubated for 2 h

(7) Eph receptor tyrosine kinases are functional entry receptors for murine gammaherpesvirus 68PLoS PathogOctober 21, 2025Anna K. Großkopf, Victor Tobiasson, Laurie T. KrugAfter three washes in 0.05% Tween20 in PBS (PBS-T), wells were blocked with 1% BSA in PBS (R&D Systems #DY995) for 1 h at room temperature. Incubation with soluble Eph proteins at the indicated concentration or

(8) Magnetic Resonance Imaging Using a Chimeric Anti-Glypican-3 Antibody Conjugated with Gadolinium Selectively Detects Glypican-3-Positive Hepatocellular Carcinoma In Vitro and In VivoCancers (Basel)October 17, 2025Yi Liu, Mingdian Tan, Mei-Sze Chua, Samuel Sominimize non-specific binding. Serum samples, diluted 1:2 to 1:5 in sample diluent (Bio-Techne, Cat# DY995, Minneapolis, MN, USA), were loaded in triplicates (50 µL per well) and incubated at 37 °C for 2 h.

(9) STING-adjuvanted outer membrane vesicle nanoparticle vaccine against Pseudomonas aeruginosaJCI InsightSeptember 9, 2025Elisabet Bjånes, Nishta Krishnan, Truman Koh, Anh T.P. Ngo, Jason Cole, Joshua Olson, Ingrid Cornax, Chih-Ho Chen, Natalie Chavarria, Samira Dahesh, et al.overnight, washed with PBST three times, and blocked with 1 x reagent diluent #2 (RD#2, R&D Systems, DY995) for 2–4 hours at room temperature (RT). Rabbit or mouse sera was serially diluted in 1 x RD#2, added

(10) Increased plasma GPNMB levels in patients with parkinson's disease and cognitive impairmentScientific reportsJuly 1, 2025Qi X, She Z, Shi X, Peng H, Wang L, Ma J, Li X, Liang K, Fan Y, Sun Y, et al.diluted at 1:30 to measure the optical density within the standard range(R&D Systems, Catalog Number: DY995). All samples were analyzed in duplicate, and the absorbance at 450 nm was determined. The intra-assay

(11) Label‐Free Single‐Molecule ImmunoassayAdvanced ScienceJune 20, 2025Xiaoyan Zhou, Chao Chen, Shuang Zhou, Guangzhong Ma, Mohammad Javad H. N. Chemerkouh, Christine L. H. Snozek, Eric H. Yang, Jiapei Jiang, Brandyn Braswell, Zijian Wan, et al.(catalog no. GRCBNPS101) were purchased from Fapon International Limited. Reagent diluent (catalog no. DY995), streptavidin conjugated horseradish peroxidase (streptavidin‐HRP, catalog no. DY998), stop solution

(12) Eph Receptor Tyrosine Kinases Are Functional Entry Receptors for Murine Gammaherpesvirus 68bioRxivJune 5, 2025Anna Katharina Grosskopf, Victor Tobiasson, Laurie T. KrugAfter three washes in 0.05% Tween20 in PBS (PBS-T), wells were blocked with 1% BSA in PBS (R&D Systems #DY995) for 1 h at room temperature. Incubation with soluble Eph proteins at the indicated concentration or

(13) On-site analysis of cortisol in saliva based on microchannel lateral flow assay (mLFA) on polymer lab-on-a-chip (LOC)Biomed MicrodevicesApril 10, 2025V. Thiyagarajan Upaassana, Supreeth Setty, Heeyeong Jang, Sthitodhi Ghosh, Chong Ahncontaining 1% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) (10 mg/mL BSA in PBS), DY995 (R&D systems, USA) was used as a blocking buffer. Phosphate-buffered saline (1X PBS), DY006 (R&D systems,

(14) ULBP2 Promotes Tumor Progression by Suppressing NKG2D-Mediated Anti-Tumor ImmunityInternational Journal of Molecular SciencesMarch 24, 2025Kohei Yamane, Kosuke Yamaguchi, Yasuhiko Teruya, Naomi Miyake, Yuji Nakayama, Takafumi Nonaka, Hiroki Chikumi, Akira Yamasakithe manufacturer’s instructions. Plasma samples from mice were diluted 1:3 with the Reagent Diluent (DY995, R&D Systems), and the culture supernatants were diluted 1:50 before being assayed. 4.4. In Vitro Proliferation

(15) Gastrointestinal Dysfunction and Low‐Grade Inflammation Associate With Enteric Neuronal Amyloid‐β in a Model for Amyloid PathologyNeurogastroenterology & MotilityMarch 6, 2025Kinga Réka Tasnády, Reindert Jehoul, Manuel Gutiérrez de Ravé, Marion J. Gijbels, Bert Brône, Ilse Dewachter, Veerle Melotte, Werend Boesmans10 min at 4°C, supernatants were collected and diluted 1:50 in sterile Reagent Diluent (R&D System, DY995), and measurements were carried out following the manufacturer's instructions (R&D Systems, DuoSet ELISA

(16) Temporal profiling of human lymphoid tissues reveals coordinated defense against viral challengeNature ImmunologyFebruary 1, 2025Matthew L. Coates, Nathan Richoz, Zewen K. Tuong, Georgina S. Bowyer, Colin Y. C. Lee, John R. Ferdinand, Eleanor Gillman, Mark McClure, Lisa Dratva, Sarah A. Teichmann, et al.1% normal donkey serum (Abcam, cat. no. ab7475) and 1% bovine serum albumin (R&D Systems, cat. no. DY995) for 1 h at 20 °C. Samples were stained for 2 h at 20 °C (primary antibodies) or 1 h at 20 °C (secondary

(17) Impact of SARS-CoV-2 vaccination and of seasonal variations on the innate immune inflammatory responseFront ImmunolJanuary 14, 2025Hend Jarras, Isalie Blais, Benjamin Goyer, Wilfried W. Bazié, Henintsoa Rabezanahary, Mathieu Thériault, Kim Santerre, Marc-André Langlois, Jean-François Masson, Joelle N. Pelletier, et al.block buffer (1% bovine serum albumin (BSA) in PBS, pH 7.2-7.4, 0.2 µm filtered, R&D Systems, Cat. DY995) at room temperature. After rinsing, 100 µL of samples, standards (31.3-2000 µg/mL) and controls were

(18) Proteolytic shedding of CD46 from human hepatocytes indicates liver stressHeliyonDecember 15, 2024Kupke P, Yang Zhou J, Glehr G, Riquelme P, Scheibert L, Adenugba A, Schlitt HJ, Geissler EK, Werner JM, Hutchinson JA.incubating at room temperature for 24 h, blocking is performed for 1 h using 300 μl/well reagent diluent (DY995, R&D Systems). Next, 100 μl/well of samples, the corresponding 1:2 serial diluted standards of rhCD46

(19) Effects of anti-inflammatory drugs on distinct endotypes of chronic rhinosinusitis without nasal polyps: comparison using an ex-vivo modelEuropean review for medical and pharmacological sciencesOctober 1, 2024Pesold VV, Wendler O, Mueller SK.

(20) Club Cell Secretory Protein-16 (CC16) as a Prognostic Biomarker for COVID-19 and H1N1 Viral InfectionsDiagnostics (Basel)August 8, 2024Shane Moore, Keerthana Gopichandran, Elizabeth Sevier, Siddhika Gamare, Sultan Almuntashiri, Gustavo Ramírez, Nora Regino, Luis Jiménez-Alvarez, Alfredo Cruz-Lagunas, Tatiana S. Rodriguez-Reyna, et al.steps were taken during the study. Human plasma samples were diluted 40 times using reagent diluent (DY995, R&D Systems). Diluted plasma samples were measured in duplicates to determine the CC16 concentration

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