AsiSI from New England Biolabs

Product Specs
ItemAsiSI
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR0630S
Concentration10,000 units/ml
Quantity500 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GCGAT/CGC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Intravenous oncolytic vaccinia expressing transgenes for enhanced safety, inhibition of shedding, imaging, and systemic cancer immunotherapyMolecular Therapy OncologyMarch 19, 2026Liliana Maruri-Avidal, Prajit Limsirichai, Nathaniel Gulizia, David H. Kirnpurified40 and digested with the restriction enzymes AscI (NEB, Ipswich, MA, R0558) and AsiSI (NEB R0630), targeting cut sites engineered into regions flanking the ΔJ2R insertion site. To construct the HSV-TK

(2) A sequence motif for DNA double-strand break and telomere healing during programmed DNA eliminationbioRxivMarch 13, 2026Jansirani Srinivasan, Marro Agbaga, Vincent Terta, Brandon Estrem, James R. Simmons, Ryan Oldridge, Mehwish Iftikhar, Abigail West, Hannah Lam, Thomas C. Dockendorff, et al.agarose plugs to protect the DNA from exogenous breaks. DSBs were blunted with exonuclease VII (NEB, Cat# R0630) and exonuclease T (NEB, Cat# M0625). Blunt ends were A-tailed and capped with END-seq adaptor 1, a

(3) Molecular interactions of Chd8 in mouse brain highlights a role in chromatin-associated RNA processingbioRxivDecember 12, 2025Emily Smith, Cesar P. Canales, Mathew W. Kenaston, Nicolas Seban, Ayanna A. Wade, Ethan Fenton, Cory Ardekani, Stephanie Lozano, Nickolas C. Chu, Daniela Perla, et al.(Thermo Scientific, K0692). The CHD8 ORF was digested using a standard restriction digest with enzyme AsiSI (NEB, R0630S). The gel purified TurboID PCR fragment and linearized CHD8 ORF clone were assembled by In-Fusion cloning

(4) Optimized chemical structure extends silencing duration of therapeutic siRNAs in dividing cancer and immune cellsMolecular TherapyNovember 5, 2025Anastasia Kremer, Tatyana Ryaykenen, Xavier Segarra-Visent, Melanie Sauer, Leon Breuer, Yiying Wu, Qi Tang, David A. Cooper, Dimas Echeverria, Clothilde Philouze, et al.TTGTAATCCAGAGGTTGATTAGCGATCGCTTACACGGCGATCTTGCC (Microsynth AG, Balgach, Switzerland). The Addgene plasmid #149718 was digested with AsiSI (R0630S, New England Biolabs) and NotI (R3189S, New England Biolabs). A 20 μl reaction containing 0,05 pmol

(5) Human genetic variation reveals FCRL3 is a lymphocyte receptor for Yersinia pestisCell GenomicsSeptember 10, 2025Rachel M. Keener, Sam Shi, Trisha Dalapati, Liuyang Wang, Nicolás M. Reinoso-Vizcaino, Micah A. Luftig, Samuel I. Miller, Timothy J. Wilson, Dennis C. Koinhibitor tablet,Sigma,Cat#11836153001 AflIII,NEB,Cat#R0541 HINDIII-HF,NEB,Cat#R3104 AsiSI,NEB,Cat#R0630 XbaI,NEB,Cat#R0145 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, Neon Transfection System 10 μL Kit

(6) Intracrine FFA4 signaling controls lipolysis at lipid dropletsNature Chemical BiologyAugust 5, 2025Shannon L. O’Brien, Emma Tripp, Natasja Barki, Elodie Blondel-Tepaz, Gabrielle Smith, Adam Boufersaoui, Jennie Roberts, Jeremy A. Pike, Joao Correia, Tamara Miljus, et al.cAMP assay (E1290) were from Promega. SNAP-Cell 647 (S9102S), BamHI (R0136), EcoRI (R3101), AsiSI (R0630) and XhoI (R0146) were from New England Biolabs. Hypergrade acetonitrile (1.00029) and GC–MS-grade SupraSolv

(7) Expanding the DNA Motif Lexicon of the Transcriptional Regulatory CodebioRxivJuly 15, 2025Jingyu Fan, Virendra K. Chaudhri, Deepa Bisht, Nicholas A. Pease, David Reed Hall, Peter Gerges, Vivian Fang Yi, Susan Kales, Ching-Huang Ho, Jishnu Das, et al.final mpra:gfp library, 10 μg of mpra:∆orf library plasmid was linearized with 100 units of AsiSI (NEB, R0630) in a final 400 μL reaction and incubated overnight at 37°C. The linearized product was then column

(8) SAMD9 senses cytosolic double-stranded nucleic acids in epithelial and mesenchymal cells to induce antiviral immunityNature communicationsApril 22, 2025Hou G, Beatty W, Ren L, Ooi YS, Son J, Zhu Y, Sheng Q, Huang W, Li D, Liu C, et al.reverse-transcribed to cDNA. Full-length SAMD9 and SAMD9L fused with 5’ AsiSI (New England Biolabs #R0630) and 3’ NotI (New England Biolabs #R0189) restriction enzyme sites were amplified using Phusion Hot Start II

(9) Systematic representation and optimization enable the inverse design of cross-species regulatory sequences in bacteriaNat CommunFebruary 19, 2025Pengcheng Zhang, Qixiu Du, Ye Wang, Lei Wei, Xiaowo Wangfor 35 cycles using primers sfGFP_F and sfGFP_R at 10 μM. The plasmid was digested with AsiSI (NEB, R0630S) for 3.5 h at 37 °C. The digested plasmid and PCR product were run on a 1% agarose gel and then purified

(10) Modeling and designing enhancers by introducing and harnessing transcription factor binding unitsNat CommunFebruary 8, 2025Jiaqi Li, Pengcheng Zhang, Xi Xi, Liyang Liu, Lei Wei, Xiaowo Wanginto the pMPRA1-oligo to create the final library. pMPRA1-oligo plasmid was digested by AsiSI (NEB, R0630). miniP-EYFP was cloned from the plasmids with NEBNext® Ultra™ II Q5® Master Mix (NEB, M0544S) for 35

(11) Massively parallel variant-to-function mapping determines functional regulatory variants of non-small cell lung cancerNat CommunFebruary 6, 2025Congcong Chen, Yang Li, Yayun Gu, Qiqi Zhai, Songwei Guo, Jun Xiang, Yuan Xie, Mingxing An, Chenmeijie Li, Na Qin, et al.primers. The purified products were then cloned into mpra:∆orf which linearized with AsiSI-HF (NEB, R0630). The final plasmid pool was electroporated as before except in ten parallel transformations followed

(12) TGF-β and RAS jointly unmask primed enhancers to drive metastasisCellOctober 31, 2024Jun Ho Lee, Francisco J. Sánchez-Rivera, Lan He, Harihar Basnet, Fei Xavier Chen, Elena Spina, Liangji Li, Carles Torner, Jason E. Chan, Dig Vijay Kumar Yarlagadda, et al.,New England Biolabs,Cat #: R3539S Esp3I,New England Biolabs,Cat #: R0734S AsiSI,New England Biolabs,Cat #: R0630S MluI-HF,New England Biolabs,Cat #: R3198S BsrGI-HF,New England Biolabs,Cat #: R3575S EcoRI-HF,New England Biolabs

(13) Somatic mosaicism in schizophrenia brains reveals prenatal mutational processesScienceOctober 11, 2024Eduardo A. Maury, Attila Jones, Vladimir Seplyarskiy, Thanh Thanh L. Nguyen, Chaggai Rosenbluh, Taejong Bae, Yifan Wang, Alexej Abyzov, Sattar Khoshkhoo, Yasmine Chahine, et al.Plasmid Midiprep Kit. Then, 20 μg of the resulting vector was then digested with 200 units of AsiSI (R0630L, NEB) in 1x CutSmart buffer in a 600-uL reaction at 37°C overnight. The linearized vector was cleaned

(14) Efficient cloning of linear DNA inserts (ECOLI) into plasmids using site-directed mutagenesisScientific ReportsSeptember 16, 2024Petra Paclikova, Jakub HarnoshDVL3 both maternal and ECFP plasmids underwent cleavage using BtgZI (NEB, #R0703) and AsiSI (NEB, #R0630S) enzymes for 2 h, first with 2 µL of AsiSI at 37 °C, then with 2 µL of BtgZI at 60 °C. AsiSI cleaved

(15) End resection and telomere healing of DNA double-strand breaks during nematode programmed DNA eliminationNucleic Acids ResearchAugust 27, 2024Brandon Estrem, Richard E Davis, Jianbin Wangmaterial. Some plugs were treated with the restriction enzymes AsiSI, FseI, AscI and/or PmeI (NEB, catalog #R0630, #R0588, #R0558 and #R0560) to generate DSBs as internal controls. DSBs were blunted with exonuclease VII

(16) Microbiota metabolism of intestinal amino acids impacts host nutrient homeostasis and physiologyCell Host & MicrobeMay 8, 2024Ting-Ting Li, Xi Chen, Da Huo, Mohammad Arifuzzaman, Shanshan Qiao, Wen-Bing Jin, Huiqing Shi, Xin V. Li, JRI Live Cell Bank Consortium, Iliyan D Iliev, et al.A4418 Arginine,GOLDBIO,Cat# A-030-500 AscI,NEW ENGLAND Biolabs,Cat# R0558L AsiSI,NEW ENGLAND Biolabs,Cat# R0630L Asparagine,Sigma-Aldrich,Cat# A0086 Aspartic acid,ACROS ORGANICS,Cat# 105041000 BBL Bacto Tryptone,BD,Cat#

(17) Mapping crossover events of mouse meiotic recombination by restriction fragment ligation-based Refresh-seqCell DiscovMarch 5, 2024Yan Wang, Yijun Chen, Junpeng Gao, Haoling Xie, Yuqing Guo, Jingwei Yang, Jun'e Liu, Zonggui Chen, Qingqing Li, Mengyao Li, et al.min, and then 65 °C 20 min to inactivate the restriction enzyme. For AsiSI (NEW ENGLAND BioLabs, cat# R0630S), the restriction digestion was performed at 37 °C 1 h, and then 80 °C 20 min to inactivate the restriction

(18) Embigin deficiency leads to delayed embryonic lung development and high neonatal mortality in miceiScienceFebruary 16, 2024Talvi S, Jokinen J, Sipilä K, Rappu P, Zhang FP, Poutanen M, Rantakari P, Heino J.from The European Conditional Mouse Mutagenesis Program, was linearized with AsiSI restriction enzyme (R0630S, NEB). The construct was then transfected by electroporation into G4 embryonic stem cells derived from

(19) Microhomology-mediated circular DNA formation from oligonucleosomal fragments during spermatogenesiseLifeOctober 17, 2023Jun Hu, Zhe Zhang, Sai Xiao, Yalei Cao, Yinghong Chen, Jiaming Weng, Hui Jiang, Wei Li, Jia-Yu Chen, Chao LiuDNA was eluted in TE buffer (10 mM Tris-Cl, pH 8.0; 1 mM EDTA, pH 8.0) and treated with AsiSI (NEB, R0630S) and PacI (NEB, R0547S) endonucleases at 37°C for genomic DNA and mtDNA fragmentation. The remaining

(20) Topoisomerase Inhibitors Increase Episomal DNA Expression by Inducing the Integration of Episomal DNA in Hepatic CellsPharmaceuticsOctober 13, 2023Andoni Gómez-Moreno, Enara San Sebastian, Jennifer Moya, Pilar Gomollón-Zueco, Sergio Isola, África Vales, Gloria González-Aseguinolaza, Carmen Unzu, Urtzi Garaigortacolumn-purified PCR product and the destination plasmid vector pLenti-C-Myc-DDK were subjected to AsiSI (cat. nº: R0630L, New England Biolabs (NEB), Ipswich, MA, USA) and MluI-HF (cat. nº: R3198L, NEB) digestion and subsequently

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AsiSI

AsiSI from New England Biolabs

Description

Sequence

New England Biolabs

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AsiSI from New England Biolabs

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